Application of antibody-conjugated small intestine submucosa to capture urine-derived stem cells for bladder repair in a rabbit model

Song, Yuting; Li, Yanqing; Tian, Mao-Xuan; Hu, Jun-Gen; Zhang, Xiuru; Liu, Pengcheng; Zhang, Xiuzhen; Zhang, Qingyi; Zhou, Li; Zhao, Lu; Li‐Ling, Jesse; Xie, Huiqi

doi:10.1016/j.bioactmat.2021.11.017

Cited by 12 publications

(9 citation statements)

References 87 publications

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“…The USCs were extracted from urine, 47 with the primary cell colonies formed within 7 days after the culture (Figure 2A ). The ACs were isolated from rabbit's auricular cartilage (Figure 2B ), and were polygon in shape after adherence.…”

Section: Resultsmentioning

confidence: 99%

Promotion of diced cartilage survival and regeneration with grafting of small intestinal submucosa loaded with urine‐derived stem cells

Li,

Wang,

Huang

et al. 2023

Cell Proliferation

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Cartilage absorption and calcification are prone to occur after the implantation of diced cartilage wrapped with autologous materials, as well as prolong the operation time, aggravate surgical trauma and postoperative pain during the acquisition process. Small intestinal submucosa (SIS) has suitable toughness and excellent degradability, which has been widely used in the clinic. Urine‐derived stem cells (USCs), as a new type of stem cells, have multi‐directional differentiation potential. In this study, we attempt to create the tissue engineering membrane material, termed USCs‐SIS (U‐SIS), and wrap the diced cartilage with it, assuming that they can promote the survival and regeneration of cartilage. In this study, after co‐culture with the SIS and U‐SIS, the proliferation, migration and chondrogenesis ability of the auricular‐derived chondrocyte cells (ACs) were significantly improved. Further, the expression levels of chondrocyte phenotype‐related genes were up‐regulated, whilst that of dedifferentiated genes was down‐regulated. The signal pathway proteins (Wnt3a and Wnt5a) were also participated in regulation of chondrogenesis. In vivo, compared with perichondrium, the diced cartilage wrapped with the SIS and U‐SIS attained higher survival rate, less calcification and absorption in both short and long terms. Particularly, USCs promoted chondrogenesis and modulated local immune responses via paracrine pathways. In conclusion, SIS have the potential to be a new choice of membrane material for diced cartilage graft. U‐SIS can enhance survival and regeneration of diced cartilage as a bioactive membrane material.

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Section: Resultsmentioning

confidence: 99%

Promotion of diced cartilage survival and regeneration with grafting of small intestinal submucosa loaded with urine‐derived stem cells

Li,

Wang,

Huang

et al. 2023

Cell Proliferation

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“…To prepare the NADC, we first modified tanezumab with ultrasmall AuQCs 28 . Typically, 20 μg tanezumab in 100 μL PBS reacted with 26.6 μM Traut's reagent at room temperature for 1 h to modify primary amines into reactive sulfhydryl groups.…”

Section: Synthesis and Characterization Of Nadcmentioning

confidence: 99%

Nanocluster-antibody-drug conjugates (NADC) as an intravesical precision theranostic agent for interstitial cystitis

Lin,

Wang,

Liu

et al. 2024

Preprint

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Interstitial cystitis (IC) is a chronic inflammatory disorder characterized by recurring severe pain in the bladder and surrounding pelvic areas, lacking timely diagnostic and therapeutic options. Here, we propose a unitary theranostic nanocluster-antibody-drug conjugate (NADC) by covalently placing dihydroorotate dehydrogenase inhibitors (DHODHi) and ultrasmall gold quantum clusters (AuQCs) on a nerve growth factor (NGF) antagonistic antibody with simultaneous X-ray computed tomographic and near-infrared fluorescence imaging contrasts. Combining anti-inflammatory effects from all individual components, intravesical NADC specifically homed to bladder mucosal lesions and capably alleviated inflammation in chronic, acute, and prophylactic IC models of rats, as revealed by behavioral and pathological evaluations. Transcriptomics unveiled cytokine modulation and concomitant inhibition of perturbed IL-17, NF-κB, TNF, and JAK-STAT signaling pathways. Interestingly, the NADC reconstructed the host bladder microbiota by differentially varying anti-inflammatory and pro-inflammatory bacteria diversities. Distinct from conventional nanoparticles conjugated with antibodies and drugs, NADC relies on the antibody framework and represents a state-of-the-art category of precision theranostic agents with translational potential for diagnosing and treating IC patients.

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“…The results of in vivo experiments indicate that AC-SIS, which are SIS scaffolds conjugated with anti-CD29 antibodies, promote rapid healing of endothelial tissue and regeneration of smooth muscle ( Fig. 5 B) [ 137 ]. Additionally, bone-marrow (BM) mesenchymal stem cells (MSCs) were co-seeded with CD34 + hematopoietic stem/progenitor cells (HSPCs) in SIS scaffolds to investigate their contribution to bladder tissue regeneration, and the results showed that increasing cell seeding density had the potential to enhance bladder tissue regeneration in the model [ 106 ].…”

Section: Tissue Repair Using Functional Sismentioning

confidence: 99%

The application of small intestinal submucosa in tissue regeneration

Zhao,

Peng,

Sun

et al. 2024

Materials Today Bio

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Application of antibody-conjugated small intestine submucosa to capture urine-derived stem cells for bladder repair in a rabbit model

Cited by 12 publications

References 87 publications

Promotion of diced cartilage survival and regeneration with grafting of small intestinal submucosa loaded with urine‐derived stem cells

Promotion of diced cartilage survival and regeneration with grafting of small intestinal submucosa loaded with urine‐derived stem cells

Nanocluster-antibody-drug conjugates (NADC) as an intravesical precision theranostic agent for interstitial cystitis

The application of small intestinal submucosa in tissue regeneration

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