Application of Atomic Absorption Spectrophotometry in the Determination of Iron in Urine

Zettner, Alfred; Mansbach, Laurence

doi:10.1093/ajcp/44.5.517

Cited by 47 publications

(30 citation statements)

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“…On the 1st day, urine was collected at hourly intervals from 8:00 a.m. until noon; sometimes an infusion of "vehicle" in 50 ml of 0.9% sodium chloride containing 0.5%o human serum albumin was given intravenously from 9:00 to 9:15 a.m. On the 2nd day, 300 USP units of purified parathyroid hormone in 50 ml of 0.9% sodium chloride and 0.5% human serum albumin were infused from 9:00 to 9:15 a.m. Urine was collected from 8:00-9:00 a.m., 9:00-9:30, 9:30-10:00, 10 :00-11 :00, and 11:00 to noon. On the 3rd day, calcium (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) Assay methods. Urine was fractionated for 3',5'-AMP as follows: to one ml of urine were added 0.03 ,uc adenosine 3',5'-monophosphate-3H, 02 ml of 8% zinc sulfate, and 0.2 ml of saturated barium hydroxide.…”

Section: Methodsmentioning

confidence: 99%

Pseudohypoparathyroidism: defective excretion of 3′,5′-AMP in response to parathyroid hormone

Chase¹,

Melson²,

Aurbach³

1969

J. Clin. Invest.

468

158

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A B S T R A C T Urinary excretion of cyclic adenosine 3',5'-monophosphate (3',5'-AMP) was tested in normal subjects and patients with pseudohypoparathyroidism, idiopathic hypoparathyroidism, surgical hypoparathyroidism, and pseudopseudohypoparathyroidism under basal conditions and after a 15 min infusion of purified parathyroid hormone. Basal excretion of the nucleotide was less than normal in the patients with hypocalcemic disorders and greater than normal in pseudopseudohypoparathyroidism. Parathyroid hormone caused a marked increase in excretion of 3',5'-AMP in all subjects except those with pseudohypoparathyroidism; nine patients with this disorder did not respond to the hormone and four showed a markedly deficient response. Radioimmunoassay showed that parathyroid hormone circulated in increased amounts in plasma from patients with pseudohypoparathyroidism and became undetectable when serum calcium was increased above 12 mg/100 ml. Suppression of parathyroid hormone secretion by induction of hypercalcemia did not alter the deficient response to exogenous hormone. The results indicate that: (a) parathyroid hormone circulates in abnormally high concentrations in pseudohypoparathyroidism and secretion of the hormone responds normally to physiological control by calcium; (b) testing urinary excretion of 3',5'-AMP in response to infusion of purified parathyroid hormone appears to-be an accurate and sensitive index for establishing the diagnosis of pseudohypoparathyroidism; and (c) the metabolic defect of the disorder can be accounted for by a lack of or defective form of parathyroid hormone-sensitive adenyl cyclase in bone and kidney.

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Section: Methodsmentioning

confidence: 99%

Pseudohypoparathyroidism: defective excretion of 3′,5′-AMP in response to parathyroid hormone

Chase¹,

Melson²,

Aurbach³

1969

J. Clin. Invest.

468

158

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“…Iron excretion was determined by atomic absorption spectrophotometry 19 based on 24-hour collections. Follow-up values represent means of three TfS indicates iron saturation of serum transferrin; MDS, myelodysplastic syndromes (classified according to French-American-British criteria); RARS, refractory anemia with ringed sideroblasts; RA, refractory anemia; RAEB, refractory anemia with excess of blasts; ERD, end-stage renal disease; DBS, Diamond-Blackfan syndrome; AML CR, acute myeloid leukemia in complete remission; and CH, chronic hemolysis of unknown etiology.…”

Section: Urinary Iron Excretionmentioning

confidence: 99%

Relationship between hepatocellular injury and transfusional iron overload prior to and during iron chelation with desferrioxamine: a study in adult patients with acquired anemias

Jensen

Christensen

et al. 2003

Blood

113

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“…Specific assays that formed the cornerstone of the clinical chemistry laboratory in the 1960s are described, such as glucose, urea, creatinine, electrolytes, cholesterol, some basic enzymes, total protein, and albumin/globulin. Of course, the contribu- Major technological milestones that have contributed to the present day clinical chemistry laboratory are listed in Table 2 (2,(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23). The analytical balance has been known since antiquity because the accurate weighing of gold and other precious materials was vitally important in ancient civilizations and contributed to the development of trade and accumulation of wealth.…”

Section: Chronicle Of the History Of Clinical Chemistrymentioning

confidence: 99%

International Year of Chemistry 2011: A Guide to the History of Clinical Chemistry

Kricka

Savory

2011

Clinical Chemistry

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BACKGROUND This review was written as part of the celebration of the International Year of Chemistry 2011. CONTENT In this review we provide a chronicle of the history of clinical chemistry, with a focus on North America. We outline major methodological advances and trace the development of professional societies and journals dedicated to clinical chemistry. This review also serves as a guide to reference materials for those interested in the history of clinical chemistry. The various resources available, in sound recordings, videos, moving images, image and document archives, museums, and websites dedicated to diagnostic company timelines, are surveyed. SUMMARY These resources provide a map of how the medical subspecialty of clinical chemistry arrived at its present state. This information will undoubtedly help visionaries to determine in which direction clinical chemistry will move in the future.

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Application of Atomic Absorption Spectrophotometry in the Determination of Iron in Urine

Cited by 47 publications

References 0 publications

Pseudohypoparathyroidism: defective excretion of 3′,5′-AMP in response to parathyroid hormone

Pseudohypoparathyroidism: defective excretion of 3′,5′-AMP in response to parathyroid hormone

Relationship between hepatocellular injury and transfusional iron overload prior to and during iron chelation with desferrioxamine: a study in adult patients with acquired anemias

International Year of Chemistry 2011: A Guide to the History of Clinical Chemistry

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