“…In addition to more conventional ones, i.e., LMs e bright field and polarized LM, fluorescence microscopy, CLSM (Dufour, 2011;El-Bakry & Sheehan, 2014;Sheen, Bao, & Cooke, 2008;van de Velde, van Riel, & Tromp, 2002), EFLM (Peighambardoust, Dadpour, & Dokouhaki, 2010) e and EMs e SEM and TEM (El-Bakry & Sheehan, 2014), cryo-SEM and cryo-TEM, STEM, ESEM and ASEM, EDX spectroscopy and microanalysis (Gatti, Tossini, Gambarelli, Montanari, & Capitani, 2009;B. James, 2009), also SPM techniques -AFM, SNOM, STM (Liu & Cheng, 2011;H. Yang et al, 2007) e have been put forward and are being increasingly applied for nanocharacterization of food.…”