In order to eliminate the zero-order and conjugate image in off-axis digital holography, a new method based on the spatial filtering of the hologram illuminated with the reference (written as RIh) wave is proposed. By using RIhand the low-pass filter to do the convolution operation, the real image of the object without the zero-order and conjugate image is directly obtained. Simultaneously, the real image always appears in the center of the reconstructed image plane. The spectrum characteristic of RIhis analyzed. A comparative analysis of the spectrum characteristics of the hologram and RIhis discussed. The theoretical and experiment results show that: this method requires only one hologram, and do once Fourier transform. The process of designing filtering window is simple and convenient.