Application of bacterial predation to mitigate recombinant bacterial populations and their DNA

Monnappa, Ajay K.; Dwidar, Mohammed; Mitchell, Robert J.

doi:10.1016/j.soilbio.2012.09.010

Cited by 37 publications

(28 citation statements)

References 42 publications

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“…Recently, Monnappa et al (2013) showed that B. bacteriovorus successfully preyed under complete anoxic conditions as long as nitrate was supplemented. Nitrite reductase and nitrite oxide reductase but not bona fide nitrate reductase genes are found in B. bacteriovorus (Rendulic et al 2004).…”

Section: Habitat and Ecologymentioning

confidence: 99%

Bdellovibrio and Like Organisms

2014

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Section: Habitat and Ecologymentioning

confidence: 99%

Bdellovibrio and Like Organisms

2014

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“…bacteriovorus HD 100 and similar predators to eradicate harmful planktonic bacteria [38] and remove preformed biofilms from surfaces [39–41], the results of this study show how this predator can be used as a preventative measure to effectively control planktonic pathogens and prevent them from establishing communities on epithelial cell surfaces. Moreover, as noted above, B .…”

Section: Discussionmentioning

confidence: 91%

“…bacteriovorus HD 100, it was grown using a top agar plate prepared using dilute nutrient agar as described previously [38]. The top layer contained E.…”

Section: Methodsmentioning

confidence: 99%

Patterning Bacterial Communities on Epithelial Cells

et al. 2013

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Micropatterning of bacteria using aqueous two phase system (ATPS) enables the localized culture and formation of physically separated bacterial communities on human epithelial cell sheets. This method was used to compare the effects of Escherichia coli strain MG1655 and an isogenic invasive counterpart that expresses the invasin (inv) gene from Yersinia pseudotuberculosis on the underlying epithelial cell layer. Large portions of the cell layer beneath the invasive strain were killed or detached while the non-invasive E. coli had no apparent effect on the epithelial cell layer over a 24 h observation period. In addition, simultaneous testing of the localized effects of three different bacterial species; E. coli MG1655, Shigella boydii KACC 10792 and Pseudomonas sp DSM 50906 on an epithelial cell layer is also demonstrated. The paper further shows the ability to use a bacterial predator, Bdellovibrio bacteriovorus HD 100, to selectively remove the E. coli, S. boydii and P. sp communities from this bacteria-patterned epithelial cell layer. Importantly, predation and removal of the P. Sp was critical for maintaining viability of the underlying epithelial cells. Although this paper focuses on a few specific cell types, the technique should be broadly applicable to understand a variety of bacteria-epithelial cell interactions.

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“…The RT-qPCR reaction conditions were as described previously (Monnappa et al, 2013). Bdellovibrio bacteriovorusextracted genomic DNA was serially diluted and used as a standard.…”

Section: Rt-qpcr Analysis To Confirm Microarray Resultsmentioning

confidence: 99%

Indole negatively impacts predation by Bdellovibrio bacteriovorus and its release from the bdelloplast

Dwidar

Nam

Mitchell

2014

Environmental Microbiology

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Bdellovibrio bacteriovorus is a predatory bacterium that attacks a wide range of Gram-negative bacterial pathogens and is proposed to be a potential living antibiotic. In this study, we evaluated the effects of indole, a bacterial signalling molecule commonly produced within the gut, on the predatory ability of B. bacteriovorus HD100. Indole significantly delayed predation on Escherichia coli MG1655 and Salmonella enterica KACC 11595 at physiological concentrations (0.25 to 1 mM) and completely inhibited predation when present at 2 mM. Microscopic analysis revealed that indole blocked the predator from attacking the prey. Furthermore, indole was not toxic to the predator but slowed down its motility. Microarray and reverse transcription quantitative polymerase chain reaction (RT-qPCR) analyses confirmed that as the gene group showing the greatest downregulation in the presence of indole was flagellar assembly genes. Indole also caused a wide spectrum changes in gene expression including general downregulation of genes involved in ribosome assembly. Furthermore, indole addition to the predatory culture after the entrance of B. bacteriovorus into the prey periplasm slowed down bdelloplast lysis. In conclusion, indole can have significant impacts on the predation efficiency, which should be taken into consideration especially if B. bacteriovorus is to be applied as a probiotic or living antibiotic.

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Application of bacterial predation to mitigate recombinant bacterial populations and their DNA

Cited by 37 publications

References 42 publications

Bdellovibrio and Like Organisms

Bdellovibrio and Like Organisms

Patterning Bacterial Communities on Epithelial Cells

Indole negatively impacts predation by Bdellovibrio bacteriovorus and its release from the bdelloplast

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