Application of Bacteriophages in the Agro-Food Sector: A Long Way Toward Approval

Fernández, Lucía; Gutiérrez, Diana; Rodrı́guez, Ana; Garcı́a, Pilar

doi:10.3389/fcimb.2018.00296

Cited by 83 publications

(54 citation statements)

References 41 publications

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“…Phage therapy represents a research field with great potential as a new and environmentally sustainable crop protection strategy (Fernández et al, 2018). Several studies have already described the in vitro efficacy of bacteriophages against different pathogenic strains of Xcc (Weiss et al, 1994;Renu et al, 2017), Dickeya solani (Hildebrandt et al, 2015), Ralstonia solanacearum (Fujiwara et al, 2011), X. campestris pv.…”

Section: Discussionmentioning

confidence: 99%

Plant Dynamic Metabolic Response to Bacteriophage Treatment After Xanthomonas campestris pv. campestris Infection

et al. 2020

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Periodic epidemics of black rot disease occur worldwide causing substantial yield losses. Xanthomonas campestris pv. campestris (Xcc) represents one of the most common bacteria able to cause the above disease in cruciferous plants such as broccoli, cabbage, cauliflower, and Arabidopsis thaliana. In agriculture, several strategies are being developed to contain the Xanthomonas infection. The use of bacteriophages could represent a valid and efficient approach to overcome this widespread phenomenon. Several studies have highlighted the potential usefulness of implementing phage therapy to control plant diseases as well as Xcc infection. In the present study, we characterized the effect of a lytic phage on the plant Brassica oleracea var. gongylodes infected with Xcc and, for the first time, the correlated plant metabolic response. The results highlighted the potential benefits of bacteriophages: reduction of bacterium proliferation, alteration of the biofilm structure and/or modulation of the plant metabolism and defense response.

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Section: Discussionmentioning

confidence: 99%

Plant Dynamic Metabolic Response to Bacteriophage Treatment After Xanthomonas campestris pv. campestris Infection

et al. 2020

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“…An important development that allowed for the re-consideration of phage therapy in the West was the publication of controlled animal studies in the English-language scientific literature in the 1980s [24][25][26] that introduced a new generation of scientists to its potential. For some time now, phage products have been approved for use in the United States to control bacterial contamination in food processing (meat, cheese) [27]. In recent years some Western European countries have begun approved therapeutic use (Belgium, France), while in the United States a number of entities are developing phage therapeutics to bring them into clinical use but these are yet to be approved by the US Food and Drug Administration.…”

Section: A Brief History Of Bacteriophage Therapymentioning

confidence: 99%

Bacteriophage Therapy: Developments and Directions

2020

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In an era of proliferating multidrug resistant bacterial infections that are exhausting the capacity of existing chemical antibiotics and in which the development of new antibiotics is significantly rarer, Western medicine must seek additional therapeutic options that can be employed to treat these infections. Among the potential antibacterial solutions are bacteriophage therapeutics, which possess very different properties from broad spectrum antibiotics that are currently the standard of care, and which can be used in combination with them and often provide synergies. In this review we summarize the state of the development of bacteriophage therapeutics and discuss potential paths to the implementation of phage therapies in contemporary medicine, focused on fixed phage cocktail therapeutics since these are likely to be the first bacteriophage products licensed for broad use in Western countries.

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“…Consequently, understanding the molecular determinants of phage-host interactions appears to be an essential step for a safe application of the therapy [14]. Recently, metabolomics analysis has suggested that the molecular response to phage infection is specific, as the molecular interactions taking place depend upon the phage and host bacterial strain [15,16]. Metabolomics is the systematic identification and quantification of all metabolites (i.e., the metabolome) in a biological matrix.…”

Section: Introductionmentioning

confidence: 99%

Bacteriophages Promote Metabolic Changes in Bacteria Biofilm

et al. 2020

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Bacterial biofilm provides bacteria with resistance and protection against conventional antimicrobial agents and the host immune system. Bacteriophages are known to move across the biofilm to make it permeable to antimicrobials. Mineral hydroxyapatite (HA) can improve the lytic activity of bacteriophages, and, together with eicosanoic acid (C20:0), can destroy the biofilm structure. Here, we demonstrate the efficacy of the combined use of phage, HA and C20:0 against Xanthomonas campestris pv campestris (Xcc) biofilm. We used nuclear magnetic resonance (NMR)-based metabolomics to investigate the molecular determinants related to the lytic action, aiming at identifying the metabolic pathways dysregulated by phage treatment. Furthermore, we identified specific markers (amino acids, lactate and galactomannan) which are involved in the control of biofilm stability. Our data show that Xccφ1, alone or in combination with HA and C20:0, interferes with the metabolic pathways involved in biofilm formation. The approach described here might be extended to other biofilm-producing bacteria.

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Application of Bacteriophages in the Agro-Food Sector: A Long Way Toward Approval

Cited by 83 publications

References 41 publications

Plant Dynamic Metabolic Response to Bacteriophage Treatment After Xanthomonas campestris pv. campestris Infection

Plant Dynamic Metabolic Response to Bacteriophage Treatment After Xanthomonas campestris pv. campestris Infection

Bacteriophage Therapy: Developments and Directions

Bacteriophages Promote Metabolic Changes in Bacteria Biofilm

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