Application of barcode high-resolution melting for rapid authentication of the medicinal plant <i>Psammosilene tunicoides</i>

Li, Jingjian; Song, Ming; Xiong, Chenglong; Zhao, Bo; Sun, Wei

doi:10.1080/13102818.2016.1181988

Cited by 15 publications

(19 citation statements)

References 27 publications

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“…HRM technology has been utilized for discriminating cultivated varieties, medicinal plant species, herbal tea, wine authenticity (Jung et al, 2010; Osathanunkul et al, 2015, 2016; Costa et al, 2016; Li et al, 2016; Song et al, 2016; Pereira et al, 2017). However, a reliable HRM approach to identify herbal medicines on Chinese herb market is still lacks.…”

Section: Resultsmentioning

confidence: 99%

Study of Commercially Available Lobelia chinensis Products Using Bar-HRM Technology

Sun

Yan

et al. 2017

Front. Plant Sci.

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There is an unmet need for herbal medicine identification using a fast, sensitive, and easy-to-use method that does not require complex infrastructure and well-trained technicians. For instance, the detection of adulterants in Lobelia chinensis herbal product has been challenging, since current detection technologies are not effective due to their own limits. High Resolution Melting (HRM) has emerged as a powerful new technology for clinical diagnosis, research in the food industry and in plant molecular biology, and this method has already highlighted the complexity of species identification. In this study, we developed a method of species specific detection of L. chinensis using HRM analysis combined with internal transcribed spacer 2. We then applied this method to commercial products purporting to contain L. chinensis. Our results demonstrated that HRM can differentiate L. chinensis from six common adulterants. HRM was proven to be a fast and accurate technique for testing the authenticity of L. chinensis in herbal products. Based on these results, a HRM approach for herbal authentication is provided.

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Section: Resultsmentioning

confidence: 99%

Study of Commercially Available Lobelia chinensis Products Using Bar-HRM Technology

Sun

Yan

et al. 2017

Front. Plant Sci.

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“…Very recently, the Bar-HRM analysis has been introduced for species identification and authentication. The use of just Bar-HRM for species authentication and detection of adulteration in commercial products has been reported [ 21 , 22 ]. To date, it has not been applied as a tool for the species authentication of Chonglou.…”

Section: Introductionmentioning

confidence: 99%

Authenticity analyses of Rhizoma Paridis using barcoding coupled with high resolution melting (Bar-HRM) analysis to control its quality for medicinal plant product

et al. 2018

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BackgroundRhizoma Paridis (Chonglou) is a commonly used and precious traditional Chinese medicine. Paris polyphylla Smith var. yunnanensis (Franch.) Hand. -Mazz. and Paris polyphylla Smith var. chinensis (Franch.) Hara are the two main sources of Chonglou under the monograph of Rhizoma Paridis in Chinese Pharmacopoeia. In the local marketplace, however, this medicine is prone to be accidentally contaminated, deliberately substituted or admixed with other species that are similar to Rhizoma Paridis in shape and color. Consequently, these adulterations might compromise quality control and result in considerable health concerns for consumers. This study aims to develop a rapid and sensitive method for accurate identification of Rhizoma Paridis and its common adulterants.MethodsDNA barcoding coupled with high resolution melting analysis was applied in this research to distinguish Rhizoma Paridis from its adulteration. The internal transcribed spacer 2 (ITS2) barcode was selected for HRM analysis to produce standard melting profile of the selected species. DNA of the tested herbal medicines was isolated and their melting profiles were generated and compared with the standard melting profile of P. polyphylla var. chinensis.ResultsThe results indicate that the ITS2 molecular regions coupled with HRM analysis can effectively differentiate nine herbal species, including two authentic origins of Chonglou and their seven common adulterants. Ten herbal medicines labeled “Chonglou” obtained from a local market were collected and identified with our methods, and their sequence information was analyzed to validate the accuracy of HRM analysis.ConclusionsDNA barcoding coupled with HRM analysis is a accurate, reliable, rapid, cost-effective and robust tool, which could contribute to the quality control of Rhizoma Paridis in the supply chain of the natural health product industry (NHP).Electronic supplementary materialThe online version of this article (10.1186/s13020-018-0162-4) contains supplementary material, which is available to authorized users.

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“…For species identification 16 , only representative samples with distinct HRM profiles need to be sequenced, thereby reducing reagent and sample consumption costs 10 – 11 . Combining HRM analysis of barcode region sequences (Bar-HRM) has been successfully used to rapidly and accurately distinguish between closely related antelope species 19 and medicinal plants 20 , 21 and to authenticate the source of vegetable oils 22 .…”

Section: Introductionmentioning

confidence: 99%

Rapid and high throughput molecular identification of diverse mosquito species by high resolution melting analysis

et al. 2016

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Mosquitoes are a diverse group of invertebrates, with members that are among the most important vectors of diseases. The correct identification of mosquitoes is paramount to the control of the diseases that they transmit. However, morphological techniques depend on the quality of the specimen and often unavailable taxonomic expertise, which may still not be able to distinguish mosquitoes among species complexes (sibling and cryptic species). High resolution melting (HRM) analyses, a closed-tube, post-polymerase chain reaction (PCR) method used to identify variations in nucleic acid sequences, has been used to differentiate species within the Anopheles gambiae and Culex pipiens complexes. We validated the use of PCR-HRM analyses to differentiate species within Anopheles and within each of six genera of culicine mosquitoes, comparing primers targeting cytochrome b ( cyt b), NADH dehydrogenase subunit 1 (ND1), intergenic spacer region (IGS) and cytochrome c oxidase subunit 1 ( COI) gene regions. HRM analyses of amplicons from all the six primer pairs successfully differentiated two or more mosquito species within one or more genera ( Aedes ( Ae. vittatus from Ae. metallicus), Culex ( Cx. tenagius from Cx. antennatus, Cx. neavei from Cx. duttoni, cryptic Cx. pipiens species), Anopheles ( An. gambiae s.s. from An. arabiensis) and Mansonia ( Ma. africana from Ma. uniformis)) based on their HRM profiles. However, PCR-HRM could not distinguish between species within Aedeomyia ( Ad. africana and Ad. furfurea), Mimomyia ( Mi. hispida and Mi. splendens) and Coquillettidia ( Cq. aurites, Cq. chrysosoma, Cq. fuscopennata, Cq. metallica, Cq. microannulatus, Cq. pseudoconopas and Cq. versicolor) genera using any of the primers. The IGS and COI barcode region primers gave the best and most definitive separation of mosquito species among anopheline and culicine mosquito genera, respectively, while the other markers may serve to confirm identifications of closely related sub-species. This approach can be employed for rapid identification of mosquitoes.

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Application of barcode high-resolution melting for rapid authentication of the medicinal plant Psammosilene tunicoides

Cited by 15 publications

References 27 publications

Study of Commercially Available Lobelia chinensis Products Using Bar-HRM Technology

Study of Commercially Available Lobelia chinensis Products Using Bar-HRM Technology

Authenticity analyses of Rhizoma Paridis using barcoding coupled with high resolution melting (Bar-HRM) analysis to control its quality for medicinal plant product

Rapid and high throughput molecular identification of diverse mosquito species by high resolution melting analysis

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