Application of biosensors with an electrolyte isolator semiconductor capacitor (EIS-CAP) transducer for process monitoring

Menzel, Christoph; Lerch, T.; Schneider, Konstantin; Weidemann, Ralf; Tollnick, C.; Kretzmer, G.; Scheper, Thomas; Schüger, K.

doi:10.1016/s0032-9592(97)00044-7

Cited by 10 publications

(2 citation statements)

References 5 publications

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“…Results obtained from the optimization studies of the crude enzyme extracts in this research, agree with the previous research which states that 𝛼-amylase are generally stable over a wide range of pH and temperature. [2,32,33] In general, enzymes are affected by changes in environmental conditions therefore, biocatalysts that remain stable without loss of activity at conditions above or below the optimum are preferred for industrial applications. It has also been reported that the enzyme source significantly determines the optimum requirements for its activity.…”

Section: Discussionmentioning

confidence: 99%

Tacca Tuber Starch—A Potential Substrate for Enzymatic Production of Glucose Syrup

et al. 2022

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Glucosesyrup obtainable from enzymatic hydrolysis of cassava and corn is widely used as sweetener in food and pharmaceutical industries. This study evaluates the potential of starch from Tacca tuber in comparison with starch from cassava tuber to produce glucose syrup using amylase from fungal isolates. Spores of amylase-producing cultures of Aspergillius niger and Rhizopus oligospora are grown on rice bran medium in solid state fermentation. Starch is obtained from Tacca (Tacca leontopetaloides) and cassava tubers using simple methods of extraction methods. Response Surface Method of the Central Composite Design version 6.0.8 is employed for optimization studies on the effect of pH, temperature, and time of hydrolysis on amylase activities of crude enzyme on the extracted starches. In comparison with cassava starch, physicochemical analysis of the glucose syrup obtained from cassava and Tacca tubers gives brix of 44 °(cloudy appearance) and 40 °(colourless in appearance) with a dextrose equivalent of 68.58 and 40.4 and specific gravity 1.200 and 1.169, respectively. An assessment of the microbiological quality of the glucose syrup produced shows no coliform or fungal growth. This study presents the potential of Tacca tuber as an economic and safe alternative to cassava starch for glucose syrup production.

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Section: Discussionmentioning

confidence: 99%

Tacca Tuber Starch—A Potential Substrate for Enzymatic Production of Glucose Syrup

et al. 2022

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“…In references [18-20], EIS biosensors consisting of a Si/SiO 2 /Si 3 N 4 /LaF 3 structure with different immobilised enzyme layers have been developed and integrated into a FIA system for the measurement of several analytes. Here, the sensor detects the changes in the fluoride-ion concentration in the sample solution resulting from the enzymatic reaction.…”

Section: Introductionmentioning

confidence: 99%

Integration of a Capacitive EIS Sensor into a FIA System for pH and Penicillin Determination

Rolka

Poghossian

Schöning

2004

Sensors

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A field-effect based capacitive EIS (electrolyte-insulator-semiconductor) sensor with a p-Si-SiO 2 -Ta 2 O 5 structure has been successfully integrated into a commercial FIA (flow-injection analysis) system and system performances have been proven and optimised for pH and penicillin detection. A flow-through cell was designed taking into account the requirement of a variable internal volume (from 12 µl up to 48 µl) as well as an easy replacement of the EIS sensor. FIA parameters (sample volume, flow rate, distance between the injection valve and the EIS sensor) have been optimised in terms of high sensitivity and reproducibility as well as a minimum dispersion of the injected sample zone. An acceptable compromise between different FIA parameters has been found. For the cell design used in this study, best results have been achieved with a flow rate of 1.4 ml/min, distance between the injection valve and the EIS sensor of 6.5 cm, probe volume of 0.75 ml, cell internal volume of 12 µl. A sample throughput of at least 15 samples/h was typically obtained.

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Bioprocess monitoring and computer control: Key roots of the current PAT initiative

Junker

Wang

2006

Biotech & Bioengineering

115

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This review article has been written for the journal, Biotechnology and Bioengineering, to commemorate the 70th birthday of Daniel I.C. Wang, who served as doctoral thesis advisor to each of the co-authors, but a decade apart. Key roots of the current PAT initiative in bioprocess monitoring and control are described, focusing on the impact of Danny Wang's research as a professor at MIT. The history of computer control and monitoring in biochemical processing has been used to identify the areas that have already benefited and those that are most likely to benefit in the future from PAT applications. Past applications have included the use of indirect estimation methods for cell density, expansion of on-line/at-line and on-line/in situ measurement techniques, and development of models and expert systems for control and optimization. Future applications are likely to encompass additional novel measurement technologies, measurements for multi-scale and disposable bioreactors, real time batch release, and more efficient data utilization to achieve process validation and continuous improvement goals. Dan Wang's substantial contributions in this arena have been one key factor in steering the PAT initiative towards realistic and attainable industrial applications.

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Application of biosensors with an electrolyte isolator semiconductor capacitor (EIS-CAP) transducer for process monitoring

Cited by 10 publications

References 5 publications

Tacca Tuber Starch—A Potential Substrate for Enzymatic Production of Glucose Syrup

Tacca Tuber Starch—A Potential Substrate for Enzymatic Production of Glucose Syrup

Integration of a Capacitive EIS Sensor into a FIA System for pH and Penicillin Determination

Bioprocess monitoring and computer control: Key roots of the current PAT initiative

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