Application of Bone Marrow-Derived Mesenchymal Stem Cells in the Treatment of Intrauterine Adhesions in Rats

Abstract

Aims: To investigate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) transplantation on intrauterine adhesions (IUA). Methods: BMSCs were isolated and labeled by green fluorescence protein. IUA model was established by mechanical injury. 48 rats were randomly divided into control, IUA model, BMSCs vein injection and BMSCs intrauterine injection groups (n=12 in each group). The third generation of BMSCs was injected through tail vein or intrauterine. Three rats were killed at time 0 h, 7 d, 14 d and 28 d and bilateral uterus were obtained at each time points for the subseqent experiments. Morphological changes were determined by hematoxylin-eosin staining or Masson staining. Estrogen receptor (ER) and progesterone receptor (PR) were detected by immunohistochemistry. Results: BMSCs were specifically stained by CD44 and CD90, but not by CD45. Before treatment, the numbers of endometrial glands were significantly decreased, while fibrosis area rate was increased in IUA model group (P<0.05 vs Control). Meanwhile, ER expression, but not PR was significantly up-regulated in model group (P<0.05 vs Control). By contrast, the therapies by BMSCs transplantation through either tail vein injection or intrauterine injection significantly elevated the numbers of endometrial glands and decreased the fibrosis area rate (P<0.05 vs Model). Moreover, both ER and PR were remarkably up-regulated after BMSCs transplantation (P<0.05 vs Model). The therapeutic effect attained to optimal level 1 or 2 weeks after transplantation. Conclusion: BMSCs transplantation was effective to repair the damaged endometrium likely through promoting the ER and PR expressions.

Sıçanlarda oluşturulan cerrahi müdahalesiz yeni bir deneysel Asherman sendromu modeli

“…Today, development of a standard treatment model for AS is a condition that researchers are studying on. There are a number of methods proposed for the AS modeling in the literature which AS modeling was carried out by endometrial scratching [10,11,21,23,25,26]. In some of these studies, it is seen that additional procedures are needed to create endometrial damage that is adequate to create IUA [25].…”

Endometrial Stem Cells in Farm Animals: Potential Role in Uterine Physiology and Pathology

“…This includes infusions of MSCs derived from bone marrow in human patients with Asherman´s syndrome [ 95 , 96 ] or through the recruitment of MSCs derived from bone marrow through CXCL12 in the same syndrome [ 97 ]. MSCs derived from bone marrow have also been used in rat models promoting the thickening of the endometrium [ 98 , 99 ], in intrauterine adhesions in rats [ 100 , 101 ], in endometrial fibrosis model in rats [ 102 ] and infusions of MSCs derived from umbilical cord in chronic endometrial damage in rats [ 103 ]. The use of such therapies in domestic species had been poorly explored.…”

Protective Effect of Taohong Siwu Decoction on Abnormal Uterine Bleeding Induced by Incomplete Medical Abortion in Rats during Early Pregnancy

“…It was reported that estrogen withdrawal triggers the influx of inflammatory cells into the endometrium 26) and tissue degradation, 27) which lead to AUB. Up-regulation of E 2 and ERα can reduce inflammatory response 29,30) and promote endometrial repair. 31) According to previous reports, the ingredients of TSD, for example, oleic and linolenic acid, 9,32) catalpol, 33) amygdalin, 34) caffeic, chlorogenic and ferulic acid, 35,36) can elevate the E 2 levels.…”