Application of Box-Behnken Experimental Design to Optimize the Extraction of Insecticidal Cry1Ac from Soil

Li, Yan-Liang; Fang, Zhixiang; You, Jing

doi:10.1021/jf304970g

Cited by 27 publications

(17 citation statements)

References 36 publications

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“…Although toxicity was detected, the LC 50 was 3 orders of magnitudes higher than concentration of Cry1Ac protein found in soil from cotton fields where Bt transgenic cottons had been cultivated for several years (0.94 ± 0.08 ng/g dw). 27 In addition to sediment bioassays, water-only toxicity testing was also conducted. Different from the sediment toxicity testing, CK exhibited slightly higher toxicity to both organisms in water than that of NC and SC, but they were still less than 20% (Figure 3).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Dissipation of Insecticidal Cry1Ac Protein and Its Toxicity to Nontarget Aquatic Organisms

Fang³

et al. 2013

J. Agric. Food Chem.

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ABSTRACT:The widespread cultivation of Bacillus thuringiensis crops has raised public concerns on their risk to nontarget organisms. Persistence of Cry1Ac protein in soil, sediment and water and its toxicity to nontarget aquatic organisms were determined. The dissipation of Cry1Ac toxin was well described using first order kinetics, with the half-lives (DT 50 ) ranging from 0.8 to 3.2, 2.1 to 7.6 and 11.0 to 15.8 d in soil, sediment and water, respectively. Microbial degradation played a key role in the dissipation of Cry1Ac toxin and high temperature accelerated the processes. Cry1Ac toxin was more toxic to the midge Chironomus dilutus than the amphipod Hyalella azteca, with the median lethal concentration (LC 50 ) of C. dilutus being 155 ng/g dry weight and 201 ng/mL in 10-d sediment and 4-d water bioassays, respectively. While Cry1Ac toxin showed toxicity to the midges, risk of Bt proteins to aquatic nontarget organisms was limited because their environmentally relevant concentrations were much lower than the LC 50 s.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…27 Briefly, after adding 1.2 mL PBST/SDS extraction solution, the soil or sediment sample was vortexed for 30 s, and the extraction was conducted at 21°C…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Dissipation of Insecticidal Cry1Ac Protein and Its Toxicity to Nontarget Aquatic Organisms

Fang³

et al. 2013

J. Agric. Food Chem.

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“…To our knowledge, this is the first study aiming to optimize the conditions of steam disinfection using the BBD. The BBD has been previously applied to optimize the removal efficiency of some heavy metals or residual chemical compounds in contaminated soil [ 38 , 39 , 40 ]. The findings of our study can be used as basic data for improving the disinfection efficiency and cost-effectiveness of high-temperature steam disinfection.…”

Section: Discussionmentioning

confidence: 99%

Optimizing Operating Parameters of High-Temperature Steam for Disinfecting Total Nematodes and Bacteria in Soil: Application of the Box−Behnken Design

Huh

Chae

Lim

et al. 2020

IJERPH

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Concerns about the widespread use of pesticides have been growing due to the adverse effects of chemicals on the environment and human health. It has prompted worldwide research into the development of a replacement to chemical disinfection of soil. The efficiency of steam sterilization, an alternative to chemical methods, has improved as technology has advanced, and the Agricultural Research and Extension Service in Korea recommends the use of steam sterilization. However, few studies have been conducted on the effects and operating conditions of high-temperature steam disinfection. In this study, we present the optimum operating conditions of a high-steam disinfector, to maximize the cost-effectiveness and removal efficiency of total nematodes and total bacteria in soil using the Box−Behnken design. The experimental data were fitted to a second-order polynomial equation using multiple regression analysis, with coefficients of determination (R2) for each model of 0.9279, 0.9678, and 0.9979. The optimum conditions were found to be a steam temperature of 150.56 °C, running speed of 1.69 m/min, and spray depth of 15.0 cm, with a corresponding desirability value of 0.8367. In the model, these conditions cause the prediction of the following responses: nematode removal efficiency of 93.99%, bacteria removal efficiency of 97.49%, and oil consumption of 70.49 mL/m2. At the optimum conditions for the steam disinfector, the removal efficiencies of nematodes and bacteria were maximized, and the oil consumption was minimized. The results of our study can be used as basic data for efficient soil disinfection using high-temperature steam.

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“…The results of a literature survey showed that different types of experimental design were used for the investigation of Co sorption by potential sorbent materials such as apatite, zeolite, bauxite residue, and dried activated sludge [23][24][25]; however, DOE approach has not been applied, to this point, for the study of Co sorption or leaching from the soil matrix. In fact, just a few examples of RSM application for the analysis of a soil remediation process can be found [26,27]. In this study, a three-level-three-factor Box-Behnken experimental design was selected for the evaluation of the effect of factors which influence the Co extraction from contaminated soil.…”

Section: Design Of Experiments (Doe)mentioning

confidence: 99%

Evaluation of Factors Affecting Chemical Extraction of Co Ions from Contaminated Soil

Smičiklas¹

2018

Statistical Approaches With Emphasis on Design of Experiments Applied to Chemical Processes

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Excessive concentrations of cobalt (Co) ions in the soil cause quality degradation and pose a significant hazard to biota. One of the options for the permanent separation of the pollutant from soil matrix is extraction by chemical reagents. In this study, responsesurface methodology (RSM) was applied to evaluate the factors affecting Co extraction from contaminated calcareous soil. Solutions of disodium ethylenediaminetetraacetate (Na 2 EDTA), citric acid (CA), and HCl were considered as leaching media. Reagent concentration, soil to solution ratio, and extraction time were selected as process variable, while Co extraction efficiencies and final pH values of extracts were the measured responses. The effect of factor variation between three levels was studied using Box-Behnken experimental design. By statistical analysis, the most influential factors were determined for each reagent, and the model equations were proposed for the prediction of system responses. Overlaid contour plots were used for the analysis of the effect of process conditions on both responses simultaneously. Given that each case of contamination is unique and requires extensive research before the remediation is implemented full-scale, it was shown that experimental design methodology is a smart approach for the assessment and comparison between the treatments.

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Application of Box-Behnken Experimental Design to Optimize the Extraction of Insecticidal Cry1Ac from Soil

Cited by 27 publications

References 36 publications

Dissipation of Insecticidal Cry1Ac Protein and Its Toxicity to Nontarget Aquatic Organisms

Dissipation of Insecticidal Cry1Ac Protein and Its Toxicity to Nontarget Aquatic Organisms

Optimizing Operating Parameters of High-Temperature Steam for Disinfecting Total Nematodes and Bacteria in Soil: Application of the Box−Behnken Design

Evaluation of Factors Affecting Chemical Extraction of Co Ions from Contaminated Soil

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