Application of buccal fat pad-derived stem cells in combination with autogenous iliac bone graft in the treatment of maxillomandibular atrophy: a preliminary human study

Khojasteh, Arash; Sadeghi, Navid

doi:10.1016/j.ijom.2016.01.003

Cited by 47 publications

(78 citation statements)

References 24 publications

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“…Despite accumulating evidence on ADSCs and their excellent potential for bone regeneration, most of the in vivo studies are in experimental animal models. Human clinical studies, which have assessed the safety and efficacy of ADSCs remain scant . In agreement with our results, ADSCs have shown beneficial results on bone healing in anterior mandibular, maxillary, and cranial bony defects.…”

Section: Discussionsupporting

confidence: 89%

“…Carlsbad, California) . All procedures intended for generating BFPSCs for autologous therapy were carried out in DMEM, containing 20% human autogenous serum, instead of xenogenic serum . Fetal bovine serum was only used for diagnostic procedures where the cells were not to be returned back to patients.…”

Section: Methodsmentioning

confidence: 99%

“…The value proposition in utilizing buccal fat pad‐derived stem cells (BFPSC) include accessibility from an intraoral source, as well as abundance of osteoprogenitor cells that enhance new bone formation . BFPSCs can simplify surgical procedures and diminish clinical risks compared to large autologous bone harvesting …”

Section: Introductionmentioning

confidence: 99%

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Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

Khojasteh

Hosseinpour

Rad

et al. 2019

Clin Implant Dent Rel Res

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Background Application of adipose‐derived stem cells originated from buccal fat pad (BFP) can simplify surgical procedures and diminish clinical risks compared to large autograft harvesting. Purpose This study sought to evaluate and compare the efficacy of buccal fat pad‐derived stem cells (BFPSCs) in combination with anorganic bovine bone mineral (ABBM) for vertical and horizontal augmentation of atrophic posterior mandibles. Materials and Methods Fourteen patients with atrophic posterior mandible were elected for this prospective exploratory study. BFP (3‐5 mL) was harvested and BFPSCs were isolated and combined with ABBM at 50% ratio. The vertical and horizontal alveolar deficiencies were augmented by 50% mixture of ABBM with either BFPSCs (group 1) or particulated autologous bone (group 2). Titanium mesh was contoured to the desired 3D shape of the alveolar ridge and fixated to the host sites over the graft material of the two groups. At first, the amount of new bone areas was calculated by quantitative analysis of cone beam computed tomography (CBCT) images that were taken 6 months postoperatively according to regenerative techniques (group 1 vs group 2 without considering the type of bone defects). Second, these amounts were calculated in each group based on the type of defects. Results Quantitative analysis of CBCT images revealed the areas of new bone formation were 169.5 ± 5.90 mm2 and 166.75 ± 10.05 mm2 in groups 1 and 2, respectively. The area of new bone formation for vertical defects were 164.91 ± 3.74 mm2 and 169.36 ± 12.09 mm2 in groups 1 and 2, respectively. The area of new bone formation for horizontal deficiencies were 170.51 ± 4.54 mm2 and 166.98 ± 9.36 mm2 in groups 1 and 2, respectively. There were no statistically significant differences between the two groups in any of the pair‐wise comparisons (P > 0.05). Conclusions The findings of the present study demonstrated lack of difference in bone volume formation between BFPSCs and autologous particulate bone in combination with ABBM. If confirmed by future large‐scale clinical trial, BFPSCs may provide an alternative to autogenous bone for reconstruction of alveolar ridge defects.

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Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

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Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

Khojasteh

Hosseinpour

Rad

et al. 2019

Clin Implant Dent Rel Res

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“…(b) Harvesting buccal fat pad with minimal discomfort for patients; permission granted from Khojasteh and Sadeghi [11]. …”

Section: Figurementioning

confidence: 99%

Buccal Fat Pad as a Potential Source of Stem Cells for Bone Regeneration: A Literature Review

Salehi

Rad

Kheiri

et al. 2017

Stem Cells International

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Adipose tissues hold great promise in bone tissue engineering since they are available in large quantities as a waste material. The buccal fat pad (BFP) is a specialized adipose tissue that is easy to harvest and contains a rich blood supply, and its harvesting causes low complications for patients. This review focuses on the characteristics and osteogenic capability of stem cells derived from BFP as a valuable cell source for bone tissue engineering. An electronic search was performed on all in vitro and in vivo studies that used stem cells from BFP for the purpose of bone tissue engineering from 2010 until 2016. This review was organized according to the PRISMA statement. Adipose-derived stem cells derived from BFP (BFPSCs) were compared with adipose tissues from other parts of the body (AdSCs). Moreover, the osteogenic capability of dedifferentiated fat cells (DFAT) derived from BFP (BFP-DFAT) has been reported in comparison with BFPSCs. BFP is an easily accessible source of stem cells that can be obtained via the oral cavity without injury to the external body surface. Comparing BFPSCs with AdSCs indicated similar cell yield, morphology, and multilineage differentiation. However, BFPSCs proliferate faster and are more prone to producing colonies than AdSCs.

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“…Moreover, BFP shows limited donor‐variation in size and is independent of body weight and fat distribution, thereby making it an attractive source of ASCs for orofacial BTE (Salehi‐Nik & Rezai Rad, ). In the present review, one research group reported RA (Khojasteh & Sadeghi, ) and AC repair (Khojasteh et al., ) using autologous BFP‐ASCs compared to AB. Superior NBF was observed in both studies when AB scaffolds were supplemented with BFP‐ASCs compared the gold standard of AB grafts alone (Khojasteh & Sadeghi, ; Khojasteh et al., ).…”

Section: Discussionmentioning

confidence: 84%

Cell therapy for orofacial bone regeneration: A systematic review and meta‐analysis

Shanbhag

Suliman

Pandis

et al. 2019

J Clinic Periodontology

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Aim The objective of the present review was to answer the focused question: what is the effect of cell therapy in terms of orofacial bone regeneration compared to grafting with only biomaterial scaffolds and/or autogenous bone? Methods Electronic databases were searched for relevant controlled clinical and pre‐clinical (large‐animal) studies. Separate meta‐analyses of quantitative data regarding histological or radiographic new bone formation were performed. Results Forty‐seven eligible clinical and 57 pre‐clinical studies were included. Clinical studies were categorized based on the use of “minimally manipulated” whole tissues (e.g., bone marrow) or ex vivo expanded cells from “uncommitted” (bone marrow, adipose tissue) or “committed” sources (periosteum, bone). Based on limited and heterogeneous clinical evidence, implantation of cells (mostly whole bone marrow), in combination with biomaterial scaffolds results in bone regeneration which is (a) superior compared to implantation of scaffolds alone in sinus and horizontal ridge augmentation, and (b) comparable to autogenous bone in alveolar cleft repair. Conclusions Although current evidence points to the benefits of cell therapy in certain clinical indications, it is unclear whether the use of ex vivo expanded cells, either uncommitted or committed, is superior to whole tissue fractions in terms of bone regeneration. The relatively larger effect sizes in favour of cell therapy observed in pre‐clinical studies are diminished in clinical trials. Future controlled studies should include cost‐effectiveness analyses to guide clinical decision‐making.

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Application of buccal fat pad-derived stem cells in combination with autogenous iliac bone graft in the treatment of maxillomandibular atrophy: a preliminary human study

Cited by 47 publications

References 24 publications

Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

Buccal fat pad‐derived stem cells with anorganic bovine bone mineral scaffold for augmentation of atrophic posterior mandible: An exploratory prospective clinical study

Buccal Fat Pad as a Potential Source of Stem Cells for Bone Regeneration: A Literature Review

Cell therapy for orofacial bone regeneration: A systematic review and meta‐analysis

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