Application of cathodoluminescence microscopy to recent and past biological materials: a decade of progress

Barbin, Vincent

doi:10.1007/s00710-013-0266-6

Cited by 60 publications

(23 citation statements)

References 57 publications

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“…Pristine shell material and subsamples altered at 100°C (Fig. A and C) show intrinsic blue cathodoluminescence colours, implying that the spatial distribution of quenching and activating elements in the crystal lattice is unchanged (ten Have & Heijnen, ; Habermann et al ., ; Barbin, ). Shells altered at 175°C mainly reveal dark blue and dull luminescence, locally with non‐luminescent portions (Fig.…”

Section: Resultsmentioning

confidence: 97%

Exploring the impact of diagenesis on (isotope) geochemical and microstructural alteration features in biogenic aragonite

et al. 2017

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For the Quaternary and Neogene, aragonitic biogenic and abiogenic carbonates are frequently exploited as archives of their environment. Conversely, pre‐Neogene aragonite is often diagenetically altered and calcite archives are studied instead. Nevertheless, the exact sequence of diagenetic processes and products is difficult to disclose from naturally altered material. Here, experiments were performed to understand biogenic aragonite alteration processes and products. Shell subsamples of the bivalve Arctica islandica were exposed to hydrothermal alteration. Thermal boundary conditions were set at 100°C, 175°C and 200°C. These comparably high temperatures were chosen to shorten experimental durations. Subsamples were exposed to different 18O‐depleted fluids for durations between two and twenty weeks. Alteration was documented using X‐ray diffraction, cathodoluminescence, fluorescence and scanning electron microscopy, as well as conventional and clumped isotope analyses. Experiments performed at 100°C show redistribution and darkening of organic matter, but lack evidence for diagenetic alteration, except in Δ47 which show the effects of annealing processes. At 175°C, valves undergo significant aragonite to calcite transformation and neomorphism. The δ18O signature supports transformation via dissolution and reprecipitation, but isotopic exchange is limited by fluid migration through the subsamples. Individual growth increments in these subsamples exhibit bright orange luminescence. At 200°C, valves are fully transformed to calcite and exhibit purple‐blue luminescence with orange bands. The δ18O and Δ47 signatures reveal exchange with the aqueous fluid, whereas δ13C remains unaltered in all experiments, indicating a carbonate‐buffered system. Clumped isotope temperatures in high‐temperature experiments show compositions in broad agreement with the measured temperature. Experimentally induced alteration patterns are comparable with individual features present in Pleistocene shells. This study represents a significant step towards sequential analysis of diagenetic features in biogenic aragonites and sheds light on reaction times and threshold limits. The limitations of a study restricted to a single test organism are acknowledged and call for refined follow‐up experiments.

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Section: Resultsmentioning

confidence: 97%

Exploring the impact of diagenesis on (isotope) geochemical and microstructural alteration features in biogenic aragonite

et al. 2017

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“…Most of the Christian Malford rostra are predominantly non-luminescent (Fig. 3C, D), consistent with them being well preserved (e.g., Barbin 2013). Thin luminescent zones on their margins and around their apical zones were avoided during subsampling.…”

Section: Fossil Preservationmentioning

confidence: 88%

Isotopic Analysis of Jurassic (Callovian) Mollusks From the Christian Malford Lagerstätte (Uk): Implications for Ocean Water Temperature Estimates Based on Belemnoids

Price

Hart

Wilby

et al. 2015

PALAIOS

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Isotopic data (C and O) derived from Callovian (Middle Jurassic) mollusks (bivalves, ammonites and belemnoids, including true belemnites and Belemnotheutis) are presented from a narrow stratigraphic interval in the Christian Malford Lagerstätte, UK. The exceptionally well-preserved mollusks include aragonite-calcite pairs precipitated by individual belemnite animals that enable an assessment of possible ''vital'' effects and the reliability of using belemnite calcite to determine ocean water compositions. The oxygen isotope data derived from the calcitic rostra of the belemnites (Cylindroteuthis) show modest variability, ranging from 21.2 to 0.9%o (V-PDB), while their accompanying aragonitic phragmocones range from 21.4 to 0.0%o. Data derived from the ammonite Kosmoceras show some scatter, with oxygen isotope values varying from 23.6 to 20.2 %o. The aragonite data from Cylindroteuthis, Kosmoceras and Belemnotheutis all overlap, suggesting they inhabited similar (surface) water depths. However, the corresponding data from the calcitic rostra of the Cylindroteuthis specimens suggest temperatures , 5uC cooler. As we have analyzed aragonite-calcite pairs, the discrepancy cannot be explained by environmental effects. Though clearly a vital effect, it is difficult to resolve whether the temperatures derived from the aragonite (phragmocone) are too warm or from the calcite (rostrum) are too cool. Consequently, the applicability of standard paleotemperature equations to Cylindroteuthid belemnite rostra remains unproven. Sequentially sampled ontogenetic isotope data derived from Belemnotheutis phragmocones reveal only modest d 18 O variation, consistent with limited movement between warmer (shallower) and cooler (deeper) waters. A coincidental systematic pattern of d 13 C enrichment may signal changes in metabolic activity associated with a shift in ecology or feeding with age.

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“…A geochemical analysis of these elements in the samples can help to test the existence of diagenetic alteration. However, the previous premise is questionable in biogenic carbonates due to the incorporation of little amounts of Mn 2þ or Fe 2þ by metabolic activity or/and by physicochemical pattern of the surrounding environment during their growth and ontogeny (Barbin et al 1991;Barbin and Gaspard 1995;Barbin 2000;Barbin 2013), and also some of biogenic carbonates can be diagenetically affected and NL, because they have no scavenged activators in the new crystals, considering that, for example, some silicification areas are NL and their surrounding alteration is also NL (unpublished results). With these reasons derived, CL is usually complemented with geochemical data (Grossman et al 1996;Rosales et al 2004).…”

Section: Characterisation Of Preservation State Of Fossil Samplesmentioning

confidence: 98%

“…In calcium carbonate material, the variation of CL is generally attributed to the concentration ratio of the activator, which is incorporated in the skeletons during digenesis (Rosales et al 2004). Pb 2þ , Mn 2þ and trivalent rare-earth elements ions are regarded as the main activators to luminescence in calcites, whereas Fe 2þ is the main inhibitor (Richter et al 2003;Flügel 2004;Barbin 2013). As a result, non-luminescent (NL) calcites are considered unaffected by diagenesis.…”

Section: Characterisation Of Preservation State Of Fossil Samplesmentioning

confidence: 98%

Computer-integrated polarisation (CIP) in the analysis of fossils: a case of study in a Palaeozoic coral (Sinopora, Syringoporicae, Carboniferous)

Coronado

Pérez‐Huerta

2014

Historical Biology

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Computer-integrated polarisation (CIP) method has been applied satisfactorily in the study of fossils skeletons of Sinopora (tabulate coral, Auloporida and Carboniferous). A previous characterisation of sample by scanning electron microscopy, atomic force microscopy and cathodoluminescence (CL) with the purpose of distinguishing the diagenetical alteration was done. Subsequently, a crystallographic comparison between CIP and electron-backscattering diffraction has been made getting a very good correlation between both methods. The CIP method allows obtaining c-axis orientation images, pole figures, and measure and mapping the misorientation of uniaxial biominerals in recent and fossil skeletons. This technique can only be used in uniaxial biominerals (calcite, quartz and hydroxylapatite), limiting its use for biaxial or bimineralic and polimineralic biominerals. CIP method has good spatial resolution (limited by camera); in our example 90 nm. The main advantage of this technique, versus other with similar properties, is the fast acquisition of data in low and high magnifications. This method does not require special treatment of samples and can be very useful for the analysis of microstructures in thin and ultra-thin sections. CIP method detects diagenetic alterations in fossil skeletons by modifications in the inner arrangement of biominerals, which combined with CL offers valuable geochemical and crystallographic information.

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Application of cathodoluminescence microscopy to recent and past biological materials: a decade of progress

Cited by 60 publications

References 57 publications

Exploring the impact of diagenesis on (isotope) geochemical and microstructural alteration features in biogenic aragonite

Exploring the impact of diagenesis on (isotope) geochemical and microstructural alteration features in biogenic aragonite

Isotopic Analysis of Jurassic (Callovian) Mollusks From the Christian Malford Lagerstätte (Uk): Implications for Ocean Water Temperature Estimates Based on Belemnoids

Computer-integrated polarisation (CIP) in the analysis of fossils: a case of study in a Palaeozoic coral (Sinopora, Syringoporicae, Carboniferous)

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