Application of cationized magnetoferritin for magnetic field-assisted delivery of short interfering RNA in vitro

Abstract

Cationized magnetoferritin is used for development of a simple, efficient, and fast delivery of short interference RNA into cells using combination of magnetophoresis for pre-concentration of siRNA-magnetoferritin complex on the surface of plated cells with subsequent application of nanosecond laser pulses producing stress waves in transfection chamber, which permeabilize cell membrane for the facilitated delivery of siRNA into the cell interior. As has been quantified using siRNA inducing cell death assay, by combination of these two physical factors we have obtained high efficiency for tested three different human carcinoma cells. Proposed method of gene silencing based on cationized magnetoferritin is a versatile and easily accessible platform with many possible applications in gene therapy.

<p>Preparation and Evaluation of Doxorubicin-Loaded PLA–PEG–FA Copolymer Containing Superparamagnetic Iron Oxide Nanoparticles (SPIONs) for Cancer Treatment: Combination Therapy with Hyperthermia and Chemotherapy</p>

“…[1][2][3] In recent years, developing nanocarrier platforms for drug delivery (chemical agent, protein, DNA, siRNA) with multifunctional ability, such as combined therapy (eg simultaneous hyperthermia and chemotherapy), targeted drug delivery and stimuli-controlled release, were considered more attractive approaches in disease treatment. 2,[4][5][6][7][8][9][10][11][12][13] Compared with conventional formulations, the therapeutic agents encapsulated in nanoparticles possess characteristics of desired solubility, enhanced bioavailability and selective biodistribution endowed by their carriers. [13][14][15][16] Among the carriers, the nanoparticles (NPs) prepared from poly (lactide) (PLA) have been extensively used due to its biocompatible, biodegradable and nontoxicity features.…”

Controlled Release of Tazarotene from Magnetically Responsive Nanofiber Patch: Towards More Efficient Topical Therapy of Psoriasis

“…For these purposes, we used nanofibers with embedded magnetic nanoparticles [27]. These nanoparticles are extensively heated when exposed to an alternating magnetic field [28][29][30][31][32][33].…”

Ultrasound-Enhanced Generation of Reactive Oxygen Species for MRI-Guided Tumor Therapy by the Fe@Fe3O4-Based Peroxidase-Mimicking Nanozyme

“…Previous studies reported that the nanozyme with peroxidase-like activity can catalyze H 2 O 2 to produce •OH via Fenton and Fenton-like reactions. − However, its therapy efficacy still remains a challenge partly due to the inadequate catalytic activity of the nanozyme and the limited amount of H 2 O 2 in the tumor. , To improve the therapy efficacy, one of the effective strategies is to regulate the concentration of H 2 O 2 by introducing different H 2 O 2 -generating agents. , For example, Shi et al designed a nanocatalyst composed of natural glucose oxidase (GOD) and ultrasmall Fe 3 O 4 integrated into silica nanoparticles, which could trigger sequential reactions to enhance therapy efficacy by using GOD to upregulate the concentration of H 2 O 2 in the tumor microenvironment . On the other hand, external stimuli, such as ultrasound, light, and magnetic field, have been applied to promote the drug release, − interfere with the RNA delivery, etc.…”

“…17,18 For example, Shi et al designed a nanocatalyst composed of natural glucose oxidase (GOD) and ultrasmall Fe 3 O 4 integrated into silica nanoparticles, which could trigger sequential reactions to enhance therapy efficacy by using GOD to upregulate the concentration of H 2 O 2 in the tumor microenvironment. 19 On the other hand, external stimuli, such as ultrasound, light, and magnetic field, have been applied to promote the drug release, 20−22 interfere with the RNA delivery, 23 etc.…”