Application of chlorophyll fluorescence performance indices to assess the wheat photosynthetic functions influenced by nitrogen deficiency

Abstract

Nitrogen deficiency influences importantly the plant photosynthetic capacity and crop productivity. Here, we employed the rapid, non-invasive measurements of chlorophyll a fluorescence kinetics for calculation of the integrative fluorescence parameters related to the leaf photosynthetic performance. In pot experiments with winter wheat (Triticum aestivum L.) we cultivated plants during the whole growing period in the soil substrate supplied with four different doses of nitrogen. The leaf nitrogen and chlorophyll content as well as the plant dry mass were analyzed after chlorophyll fluorescence records in three growth stages. Our results indicate that the commonly used parameter F v /F m (the maximum quantum yield of photochemistry) was almost insensitive to nitrogen treatment. In contrary, the performance index (PI abs ) and total performance index (PI tot ) were much more responsive and significant differences among plants of different nitrogen treatments as well as between the youngest and third leaf from the top were observed. Parameter PI tot was shown to express only small diurnal changes, thus being more reliable and more useful for comparison of different samples in field conditions than more frequently used parameter PI abs .

Assessing the Reliability of Thermal and Optical Imaging Techniques for Detecting Crop Water Status under Different Nitrogen Levels

“…In abiotic stress studies (e.g., drought and nitrogen deficiency), the chlorophyll a fluorescence and the derived indices have been successfully used to assess the plant stress conditions [25][26][27]. In this study, chlorophyll a fluorescence was measured with the portable fluorometer "Handy-PEA" (Hansatech, Norfolk, UK).…”

A User’s View of the Parameters Derived from the Induction Curves of Maximal Chlorophyll a Fluorescence: Perspectives for Analyzing Stress

“…It is near to impossible not to evoke the popular Performance Index (PI) of Strasser when discussing the parameters derived from the JIP-test (Silvestre et al, 2014; Zivčák et al, 2014). Recently Kalaji et al (2016) recommended the non-specialist to resort to the PI in the absence of a serious capacity to understand and exploit the other parameters.…”

Using Different Regression Methods to Estimate Leaf Nitrogen Content in Rice by Fusing Hyperspectral LiDAR Data and Laser-Induced Chlorophyll Fluorescence Data

“…Fluorescence ratios (F 740 /F 685 and F 685 /F 525 ) were widely utilized for the estimation of the nitrogen status and for the diagnosis of crop growth because of their simplicity in measurement and interpretation [11,46]. However, studies of Yang Jian et al [47] indicated that these fluorescence ratios were insensitive to the rice LNC.…”