Application of chromatography in purification and structural analysis of natural polysaccharides: A review

Zheng, Yi; Yan, Jingyu; Cao, Cuiyan; Liu, Yanfang; Yu, Dongping; Liang, Xinmiao

doi:10.1002/jssc.202300368

Cited by 11 publications

(1 citation statement)

References 158 publications

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“…The more commonly used anion exchange columns for the purification of sea buckthorn polysaccharides are DEAE-cellulose column and DEAE-agarose column. Gel column chromatography is to further purify the polysaccharides according to the characteristics of molecular sieves specific to different gels [51] . The commonly used gel chromatographic columns for purification of sea buckthorn polysaccharides are agarose gel column, dextran gel column and acryl dextran gel column.…”

Section: Purification Methodsmentioning

confidence: 99%

Prospects of sea buckthorn ( Hippophae rhamnoides L.) polysaccharides: preparation, structural characterization, and bioactivities diversity

Shi,

Wu,

Han

et al. 2024

Food Science and Human Wellness

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Sea buckthorn (Hippophae rhamnoides L.) is a natural homologous substance of medicine and food. Polysaccharide, as one of its primary active components, has very superior biological activity and can be used as a dietary supplement for functional foods, with good commercial prospects. Although initial progress has been made in the study of sea buckthorn polysaccharides, related studies have been fragmented and lacked systematic and generalization. This manuscript presents a critical analysis and systematic summary of the extraction and purification methods, structural characterization and physicochemical properties, biological activity and potential mechanisms, and structure-activity relationships of sea buckthorn polysaccharides. Accumulating evidence has indicated that sea buckthorn polysaccharides, which were widely prepared by water extraction and column chromatography purifications, exhibited exhibit superior biological activities in vitro and in vivo, including antioxidant, immunomodulatory, anti-inflammatory, hepatorenal protective, antibacterial, antiviral, and prebiotic activities. After analysis, it was concluded that there is a correlation between the relevant activities of sea buckthorn polysaccharides and that the structure of sea buckthorn polysaccharides has a great influence on their biological activity. We reviewed the challenges and limitations of sea buckthorn polysaccharides, summarized the critical aspects, and provided suggestions for potential breakthroughs in the research and application of sea buckthorn polysaccharide.

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Section: Purification Methodsmentioning

confidence: 99%

Prospects of sea buckthorn ( Hippophae rhamnoides L.) polysaccharides: preparation, structural characterization, and bioactivities diversity

Shi,

Wu,

Han

et al. 2024

Food Science and Human Wellness

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Effects of Separation and Purification Methods on Antioxidation, Hypoglycemic and DNA Protection Activity of Fenugreek Polysaccharide

Wang,

Feng,

Liang

et al. 2024

Chemistry & Biodiversity

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Six low molecular weight fenugreek polysaccharides (FP) were isolated and purified by ethanol stepwise precipitation (EFP‐20, EFP‐40, and EFP‐60) and DEAE‐52 cellulose column method (DFP‐0, DFP‐0.15, and DFP‐0.3), respectively. The effects of different separation and purification techniques on the preliminary properties and biological activities of fenugreek polysaccharides were compared. The results showed that the DEAE‐52 cellulose‐eluted fractions had a higher total sugar content and displayed a looser structure. The molecular weights of all six fractions were in the range of 4‐19 kDa, with significant changes in the ratio of galactose to mannose. All six fractions contained α‐D‐galactopyranose and β‐D‐mannopyranose structures. Activity tests showed that all six fractions possessed antioxidant, hypoglycemic and DNA‐protective activities. Among them, the DFP‐0 fraction showed the highest activity. Overall, different isolation and purification methods lead to changes in the properties and bioactivities of FP, which provides a theoretical basis for the development and application of FP in functional foods and drugs.

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Purification, Source Exploration, and Bioactivity Evaluation of Oligosaccharides in Sijunzi Decoction

Chen,

Pu,

Liu

et al. 2024

J of Separation Science

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Sijunzi decoction (SJZD) has been widely used to treat splenic deficiency syndrome. Previous studies confirmed that polysaccharides and non‐polysaccharides (NPS) are the main active components of SJZD. This study aimed to investigate the composition and activity of oligosaccharides in NPS. In this study, the oligosaccharide component (named SJZD‐OGS), consisting of several different oligosaccharides, was separated and purified from non‐polysaccharides of SJZD (SJZD_NPS). Ultra‐high‐performance liquid chromatography–ion mobility spectrometry–quadrupole time‐of‐flight mass spectrometry (UHPLC–IMS–QTOF/MS), matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF‐MS), and high‐performance anion‐exchange chromatography with pulsed amperometric detection (HPAEC‐PAD) were used to analyze SJZD‐OGS. SJZD‐OGS, with a molecular weight of 2178 Da, was composed of glucose and fructose, and its chemical composition mainly included fructo‐oligosaccharides (FOS) with a degree of polymerization of 3–14 (DP 3–14). The results of oligosaccharide source exploration demonstrated that the chemical composition of SJZD‐OGS was different from that of the oligosaccharides of each herb in SJZD. In addition, SJZD‐OGS showed good anti‐inflammatory activity on macrophage cells and intestinal epithelial cell protective activity. The present work provides experimental evidence for elucidating the pharmaceutical components of SJZD and presents an effective strategy for the research of oligosaccharides in traditional Chinese medicine (TCM).

show abstract

Application of chromatography in purification and structural analysis of natural polysaccharides: A review

Cited by 11 publications

References 158 publications

Prospects of sea buckthorn ( Hippophae rhamnoides L.) polysaccharides: preparation, structural characterization, and bioactivities diversity

Prospects of sea buckthorn ( Hippophae rhamnoides L.) polysaccharides: preparation, structural characterization, and bioactivities diversity

Effects of Separation and Purification Methods on Antioxidation, Hypoglycemic and DNA Protection Activity of Fenugreek Polysaccharide

Purification, Source Exploration, and Bioactivity Evaluation of Oligosaccharides in Sijunzi Decoction

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