Application of Cloud Point Method for Spectrophotometric Determination of Salbutamol Sulphate and Methyldopa

Abstract

A simple and efficient cloud point spectrophotometric method has been used for the determination of salbutamol sulphate and methyldopa both in pure and pharmaceutical preparations. The procedure was based on the ion association formation with eosin Y. The extraction of ion association, drown to Triton X-114 micelles, was measured spectrophotometrically. The phase separation was studied and optimized. Beer's law was rectilinear over the concentration ranges of 0.1-20 and 0.3-10 µg/mL with molar absorptivity 4x10 4 and 5.7x10 4 L.mol -1 cm -1 and average recovery 98.21% and 101.27% for the above drugs, respectively. The method was applied successfully for the determination of salbutamol sulphate and methyldopa in pharmaceuticals.

Development of a Sensitive Spectrophotometric Determination of Methyldopa Drug

“…There are several analytical methods that have been published for the analysis of methyldopa in biological fluids, pharmaceutical form, or bulk. High-performance liquid chromatography with fluorescence detection [5], colorimetry [6,7], GLC [8], titrimetry [9] electrophoresis [10] ,NMR [11] ,thin layer [12],voltametry [13,14], spectrophotometry [15][16][17][18][19][20][21] and flow injection spectrophotometry [22][23][24][25] are a few of these methods. Some of these techniques, meanwhile, take a long time and/or need for expensive tools and setups.…”

A New Visible Spectrophotometric Approach for Determination of Methyldopa in Pharmaceuticals

“…For the analysis of methyldopa in bulk, pharmaceutical form, or biological fluids, a range of analytical techniques has been published. HPLC 7 , HILIC-MS/MS 8 , electrochemical 9,10 , electrophoresis 11 , NMR 12 , mass spectrometry 13 , voltammetry 14,15 , cloud point analysis 16,17 and spectrophotometry [18][19][20][21][22] are some of the techniques used. However, several of these approaches take time and/or necessitate costly equipment and circumstances.…”

Spectrophotometric Determination of Catecholamine Containing Drugs Using Calcon Dye

“…Various spectrophotometric methods using different reagents have been reported for the determination of catecholamine drugs. These methods include ion association using eosin Y and application of cloud point extraction technique [26], charge transfer complexes using bromanil [27], oxidation-reduction using ferric ion [28][29][30], diazotisation using pnitroaniline [31], 4-aminoantipyrine in the basic medium [32], complexation reaction using 4-aminoantipyrine and copper ion, ionpair complex using triiodide ion [33] and using ferrous ion as a complexing agent in alkaline medium [34], oxidative coupling using p-toluidine in the presence of sodium periodide [35], 2, 6-diaminopyridine in the presence of potassium periodate [36] and 3methyl-2-benzothiazolinone hydrazone hydrochloride monohydrate in the presence of potassium ferricyanide [37].…”