Application of combined gas-liquid chromatography-mass spectrometry to the identification of sterols in oat seed

High Performance Liquid Chromatography of Molecular Species from Free Sterols and Sterylglycosides Isolated from Oat Leaves and Seeds1

“…These HPLC-results are confirmed by GLC-analysis ( Fig. lb and 2b) and are in accordance with Knights (1965) and Knights and Laurie (1967). HPLC-separation of free sterols shows coelution of J 7 -avenasterol/cholesterol and J 7 -stigmastenol/stigmasterol, thus confirming that GLC still is the method of choice for the analysis of free sterols.…”

Lipid Biochemistry of Fungi and Other Organisms

“…Although stigmast-7-enol (Jeong et al, 1974) and stigmasta-7,24(28)-dienol (Knights and Laurie, 1967) are minor sterols of some higher plants, the fact that the "typical" higher plant sterols cholesterol, campesterol, stigmasterol, and sitosterol are absent from the spores suggests that rust and smut sterols are true fungal products. Furthermore, germinating bean rust uredospores synthesize the ~7 and ~7,24(28) C29 sterols from radio labeled substrates (Lin and Knoche, 1974).…”

Saturated Sterols (Stanols) in Unhydrogenated and Hydrogenated Edible Vegetable Oils and in Cereal Lipids*

“…No saturated sterols could be quantified in oat lipids under the present G C system, however a trace amount of sitostanol (C29) was detected by GC-MS operated in the MID mode (results not presented). The presence of both C28 and C29 saturated sterols was also reported in oat lipids (Knights and Laurie 1967), although no quantitative data were presented.…”