Application of commercial enzyme linked immunosorbent assays (ELISA) for the detection of antibodies for foot-and-mouth disease virus in wild boar and red deer

Abstract

For detecting antibodies towards foot and mouth (FMD) virus in sera collected from red deer hinds (Cervus elaphus) and wild boars (Sus scrofa), three commercially available enzyme-linked immunosorbent assays (ELISA) were used. Two ELISA kits (PrioCHECK FMDV NS and CHEKIT FMD-3ABC) were used for the detection of antibodies towards non-structural proteins of FMD virus and one assay was based on the detection of antibodies for serotype O (PrioCHECK FMDV type O). All of the sera tested in our study were negative for antibodies against FMD virus. The aim of this study was to investigate the usefulness of commercially available ELISA kits given for marketing authorization in Croatia in testing the prevalence of FMD antibodies in wild boar and red deer populations. Since the producers of ELISA kits used in our study did not declare wild animals as a target species, we hypothesised that the same kits could be used for serological diagnosis of FMD in red deer and wild boars. Our study confirmed that the kits used are acceptable for detecting antibodies in both species tested, however, the investigation highlighted the problem of validating the kits due to the absence of available positive sera originating from red deer, as well as other susceptible species, especially artiodactyls