Application of commercially available fenitrothion-ELISA kit for soil residue analysis

Conde, Sonny; Suyama, Kousuke; Itoh, Kazuhito; Yamamoto, Hiroki

doi:10.1584/jpestics.g07-21

Cited by 6 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S. leopoliensis CCAP1405/1 was purchased from the Culture Collection of Algae and Protozoa (Oban, UK) in 2005, and has been subcultured in CT liquid medium (Ca(NO3)2·4H2O 0.150 g, KNO3 0.1 g, β-Na2 glycerophosphate·nH2O 0.05 g, MgSO4·7H2O 0.04 g, vitamin B12 0.1 μg, biotin 0.1 μg, thiamine HCl 10 μg, PIV metal solution 3 mL, N-tris(hydroxymethyl) methyl-3-aminopropanesulfonic acid (TAPS) 0. 4 (34,35) under the conditions described above, but pink colonies appeared with the green colonies of S. leopoliensis CCAP1405/1 on the plates at all times (Fig. 1).…”

Section: Microorganismsmentioning

confidence: 79%

Growth of the Cyanobacterium Synechococcus leopoliensis CCAP1405/1 on Agar Media in the Presence of Heterotrophic Bacteria

2011

Self Cite

View full text Add to dashboard Cite

The cyanobacterium Synechococcus leopoliensis CCAP1405/1 does not grow on common solid media made of agar, agarose HT, noble agar, gelrite and gelatin, although it grows in liquid media with the same components. The inoculation of S. leopoliensis CCAP1405/1 at a high initial cell density allowed it to grow on the agar media, and co-inoculation with one of the heterotrophic bacterial strains belonging to a wide range of phylogeny, showed the same effects even at a low initial cell density of S. leopoliensis CCAP1405/1. The addition of thiosulfate and high concentrations of vitamin B12, biotin and thiamine also supported growth on solid media, but catalase had no effect. On inorganic solid media, the autotrophic cyanobacterial strain supported the growth of heterotrophic bacteria, suggesting mutual interaction.

show abstract

Section: Microorganismsmentioning

confidence: 79%

Growth of the Cyanobacterium Synechococcus leopoliensis CCAP1405/1 on Agar Media in the Presence of Heterotrophic Bacteria

2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…In our previous work, overestimation of recovery occurred at 10 times dilution, 3) therefore, we examined the effect of higher diluted extracts on the recovery using a more sensitive ELISA plate. The recovery of fenitrothion from soil samples is shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Five soils were selected based on the results of previous study in which samples had overestimated (Ͼ120%) fenitrothion recoveries, 3) these were Honjo B, Izumo, Koryo, Jinzai, and Takano A.…”

Section: Soil Samplesmentioning

confidence: 99%

“…1,2) In our previous study, we used a commercially available fenitrothion-ELISA kit, designed for residue analysis of crop samples, for soil residue analysis, but recovery by ELISA was highly biased in some soil samples, and it was suggested that soil matrices, such as high-molecular-weight organics and divalent cations, influenced the ELISA reaction to overestimate recovery. 3) Zeolites are inorganic materials with large surface areas and well-defined internal structures of uniform cages, cavities or channels. Chemically, the compound is a crystalline aluminosilicate hydrate containing alkaline metal or alkaline earth metal.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improvement of ELISA analysis for soil residue analysis by reducing soil matrix effects

2008

Self Cite

View full text Add to dashboard Cite

“…It is well known that antibodies can bind to specific antigens and this ability has been used as the basis for many assays [19][20][21]. This specific recognition and binding has been utilized in various techniques including the enzyme-linked immunosorbent assay (ELISA).…”

Section: Introductionmentioning

confidence: 99%

The Development and Use of an Elisa-Based Method to Follow the Distribution of Cellulase Monocomponents During the Hydrolysis of Pretreated Corn Stover

Pribowo¹,

Hu²,

Arantes³

et al. 2015

Fuel Production From Non-Food Biomass

View full text Add to dashboard Cite

Background: It is widely recognised that fast, effective hydrolysis of pretreated lignocellulosic substrates requires the synergistic action of multiple types of hydrolytic and some non-hydrolytic proteins. However, due to the complexity of the enzyme mixture, the enzymes interaction with and interference from the substrate and a lack of specific methods to follow the distribution of individual enzymes during hydrolysis, most of enzyme-substrate interaction studies have used purified enzymes and pure cellulose model substrates. As the enzymes present in a typical "cellulase mixture" need to work cooperatively to achieve effective hydrolysis, the action of one enzyme is likely to influence the behaviour of others. The action of the enzymes will be further influenced by the nature of the lignocellulosic substrate. Therefore, it would be beneficial if a method could be developed that allowed us to follow some of the individual enzymes present in a cellulase mixture during hydrolysis of more commercially realistic biomass substrates. Results: A high throughput immunoassay that could quantitatively and specifically follow individual cellulase enzymes during hydrolysis was developed. Using monoclonal and polyclonal antibodies (MAb and PAb, respectively), a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was developed to specifically quantify cellulase enzymes from Trichoderma reesei: cellobiohydrolase I (Cel7A), cellobiohydrolase II (Cel6A), and endoglucanase I (Cel7B). The interference from substrate materials present in lignocellulosic supernatants could be minimized by dilution. Conclusion: A double-antibody sandwich ELISA was able to detect and quantify individual enzymes when present in cellulase mixtures. The assay was sensitive over a range of relatively low enzyme concentration (0 -1 μg/ml), provided the enzymes were first pH adjusted and heat treated to increase their antigenicity. The immunoassay was employed to quantitatively monitor the adsorption of cellulase monocomponents, Cel7A, Cel6A, and Cel7B, that were present in both Celluclast and Accellerase 1000, during the hydrolysis of steam-pretreated corn stover (SPCS). All three enzymes exhibited different individual adsorption profiles. The specific and quantitative adsorption profiles observed with the ELISA method were in agreement with earlier work where more labour intensive enzyme assay techniques were used.

show abstract

Application of commercially available fenitrothion-ELISA kit for soil residue analysis

Cited by 6 publications

References 11 publications

Growth of the Cyanobacterium Synechococcus leopoliensis CCAP1405/1 on Agar Media in the Presence of Heterotrophic Bacteria

Growth of the Cyanobacterium Synechococcus leopoliensis CCAP1405/1 on Agar Media in the Presence of Heterotrophic Bacteria

Improvement of ELISA analysis for soil residue analysis by reducing soil matrix effects

The Development and Use of an Elisa-Based Method to Follow the Distribution of Cellulase Monocomponents During the Hydrolysis of Pretreated Corn Stover

Contact Info

Product

Resources

About