Application of Cord Blood and Cord Blood-Derived Induced Pluripotent Stem Cells for Cartilage Regeneration

Rim, Yeri Alice; Nam, Yoonho

doi:10.1177/0963689718794864

Cited by 18 publications

(14 citation statements)

References 81 publications

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“…In our study, a relatively low chondrogenic induction could be related to the low percentage of KSR (5%) and shorter period of differentiation in 3D culture. The recent study of Nam’s group reprogrammed cord-blood derived cells in iPSC and 47 days (30 days in 3D culture) and 20% KSR presence in the medium were sufficient for the induction of well-formed cartilage-like spheres [24,51,52]. It is worth mentioning that in their approach the induction of MSC from PSC cells was performed via the 20% FBS supplementation of monolayer culture of EB outgrowth.…”

Section: Discussionmentioning

confidence: 99%

Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions

Lach

Wróblewska

Kulcenty

et al. 2019

IJMS

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The repair of damaged articular cartilage using currently available implantation techniques is not sufficient for the full recovery of patients. Pluripotent stem cells (iPSC)-based therapies could bring new perspectives in the treatment of joint diseases. A number of protocols of in vitro differentiation of iPSC in chondrocytes for regenerative purposes have been recently described. However, in order to use these cells in clinics, the elimination of animal serum and feeder cells is essential. In our study, a strictly defined and controllable protocol was designed for the differentiation of pluripotent stem cells (BG01V, ND 41658*H, GPCCi001-A) in chondrocyte-like cells in serum- and a feeder cell-free system, using the embryoid bodies step. The extension of the protocol and culture conditions (monolayer versus 3D culture) was also tested after the initial 21 days of chondrogenic differentiation. Promotion of the chondrogenic differentiation in 3D culture via the elevated expression of genes related to chondrogenesis was achieved. Using immunofluorescence and immunohistochemistry staining techniques, the increased deposition of the specific extracellular matrix was indicated. As a result, chondrocyte-like cells in the early stages of their differentiation using pellet culture under fully controlled and defined conditions were obtained.

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Section: Discussionmentioning

confidence: 99%

Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions

Lach

Wróblewska

Kulcenty

et al. 2019

IJMS

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“…The simple yet complicated architecture of the articular cartilage compromises its natural capacity for self-repair [1]. The cartilage, with an avascular and alymphatic nature, is composed of dense layers of extracellular matrix (ECM) containing embedded distributed chondrocytes [2]. Cartilage lines the surface of the bone and provides a low-friction surface by absorbing external pressure or stimuli, which enables painless joint movement [3].…”

Section: Introductionmentioning

confidence: 99%

The Role of Chondrocyte Hypertrophy and Senescence in Osteoarthritis Initiation and Progression

Rim

Nam

2020

IJMS

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229

141

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Osteoarthritis (OA) is the most common joint disease that causes pain and disability in the adult population. OA is primarily caused by trauma induced by an external force or by age-related cartilage damage. Chondrocyte hypertrophy or chondrocyte senescence is thought to play a role in the initiation and progression of OA. Although chondrocyte hypertrophy and cell death are both crucial steps during the natural process of endochondral bone formation, the abnormal activation of these two processes after injury or during aging seems to accelerate the progression of OA. However, the exact mechanisms of OA progression and these two processes remain poorly understood. Chondrocyte senescence and hypertrophy during OA share various markers and processes. In this study, we reviewed the changes that occur during chondrocyte hypertrophy or senescence in OA and the attempts that were made to regulate them. Regulation of hypertrophic or senescent chondrocytes might be a potential therapeutic target to slow down or stop OA progression; thus, a better understanding of the processes is required for management.

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“…Zhang et al (30) indicated that the Wnt/β-catenin and TNF receptor superfamily of proteins were associated with the survival rate of UCBSCs, while inhibition of bone morphogenetic protein markers may prevent their independent differentiation. Several studies have produced feeder-free culture media, such as TeSRl that contains certain recombinant growth factors, which aim to enhance the ability of UCBSCs to regenerate (31,32). Zhou et al (33) demonstrated that the combination of activin inhibitors and MEK/ERK inhibitors increased the induction rate of UCBSCs.…”

Section: Differentiation Of Ucbscsmentioning

confidence: 99%

DNA barcode to trace the development and differentiation of cord blood stem cells (Review)

et al. 2021

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Umbilical cord blood transplantation was first reported in 1980. Since then, additional research has indicated that umbilical cord blood stem cells (UCBSCs) have various advantages, such as multi-lineage differentiation potential and potent renewal activity, which may be induced to promote their differentiation into a variety of seed cells for tissue engineering and the treatment of clinical and metabolic diseases. Recent studies suggested that UCBSCs are able to differentiate into nerve cells, chondrocytes, hepatocyte-like cells, fat cells and osteoblasts. The culture of UCBSCs has developed from feeder-layer to feeder-free culture systems.The classical techniques of cell labeling and tracing by gene transfection and fluorescent dye and nucleic acid analogs have evolved to DNA barcode technology mediated by transposon/retrovirus, cyclization recombination-recombinase and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 strategies. DNA barcoding for cell development tracing has advanced to include single cells and single nucleic acid mutations. In the present study, the latest research findings on the development and differentiation, culture techniques and labeling and tracing of UCBSCs are reviewed. The present study may increase the current understanding of UCBSC biology and its clinical applications.

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Application of Cord Blood and Cord Blood-Derived Induced Pluripotent Stem Cells for Cartilage Regeneration

Cited by 18 publications

References 81 publications

Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions

Chondrogenic Differentiation of Pluripotent Stem Cells under Controllable Serum-Free Conditions

The Role of Chondrocyte Hypertrophy and Senescence in Osteoarthritis Initiation and Progression

DNA barcode to trace the development and differentiation of cord blood stem cells (Review)

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