Objective: This study aimed to investigate the clinical efficacy of a cortical bone trajectory (CBT) screw technique combined with an anterior small incision lesion removal and bone grafting for the treatment of single-segment lumbar spinal tuberculosis. Methods: A retrospective analysis of 42 patients with lumbar tuberculosis was performed. The patients were divided into two groups: intervertebral CBT screw fixation group (CBT group) and posterior pedicle screw fixation group (traditional group). Then the surgery time, bleeding volume, postoperative drainage volume, postoperative hospital stay, visual analogue scale (VAS) score, Cobb angle, neurological function (Frankel grading), and bone graft fusion time were compared between the two groups. Results: There were no significant differences between the two groups in terms of the operation times, bleeding volumes, drainage volumes, hospital stays, VAS scores, and Frankel grading. VAS scores, Cobb angles, and Frankel grading improved significantly after surgeries, without a significant difference between the two groups. At the final follow-up, no significant loss of the Cobb angle was found. All of the patients were followed up for 14 to 30 months, with a mean time of 20.8 months. The bone graft fusion time ranged from 3 to 8 months, with a mean time of 5.1 months. There were no serious complications. Conclusions: The CBT screw technique combined with an anterior small incision lesion removal and bone grafting is an effective and safe method for the treatment of lumbar tuberculosis.