Application of coupled oxidation reaction to electron microscopic demonstration of horseradish peroxidase: cobalt-glucose oxidase method

Itoh, Kazuyoshi; Konishi, Akira; Nomura, Sakashi; Mizuno, Noboru; Nakamura, Yasuhisa; Sugimoto, Tetsuo

doi:10.1016/0006-8993(79)91013-8

Cited by 526 publications

(112 citation statements)

References 24 publications

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“…Between each incubation, the sections were rinsed thoroughly with a Tris-buffer solution containing Triton-X100 at pH 7.4. After incubation, peroxidase was visualized by the cobalt-glucose oxidase method with diaminobenzidine (DAB) (Itoh et aL, 1979). After mounting on gelatin-coated slides, the sections were dehydrated in alcohol, cleared in xylene, coverslipped, and observed under a light microscope.…”

Section: Methodsmentioning

confidence: 99%

Ascending Projections from the Area Postrema and the Nucleus of the Solitary Tract of Suncus Murinus

Ito

Seki

1998

Okajimas Folia Anatomica Japonica

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Summary: Suncus murinus (suncus) is a new experimental animal model for research on the mechanisms underlying emesis. In the present study, we examined the ascending projections from the area postrema (AP) and the nucleus of the solitary tract (NTS) in suncus based on anterograde transport of phaseolus vulgaris leucoagglutinin. The AP projected heavily to the dorsal vagal complex, especially in the commissural and medial subnuclei of the NTS, and the dorsal motor nucleus of the vagus. Some ascending fibers from the AP projected bilaterally to the parabrachial nucleus (Pb), but no labeling was observed rostral to this area. In contrast, the NTS had extensive projections as far as the basal forebrain. The NTS projections were observed in the AP, ventrolateral reticular formation including the nucleus ambiguus, A5 noradrenergic area, locus coeruleus, Pb, and central gray matter of the midbrain. At the level of the diencephalon, the NTS projections were seen in the dorsomedial, lateral, paraventricular, periventricular, supraoptic, retrochiasmatic and arcuate nuclei of the hypothalamus, in addition to the paraventricular nucleus of the thalamus. Terminal fields within the basal forebrain were also shown to include the medial preoptic area, the bed nucleus of the stria terminalis, the substantia innominata and the ventral pallidum.The results indicated that the neurological relationship between the chemo-and/or barosensitive systems including the trigger of the emetic response and the general viscerosensory and/or -motor systems may exist also in the suncus.

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Section: Methodsmentioning

confidence: 99%

Ascending Projections from the Area Postrema and the Nucleus of the Solitary Tract of Suncus Murinus

Ito

Seki

1998

Okajimas Folia Anatomica Japonica

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“…After incubation in the primary antiserum, sections were incubated with biotinylated second antibodies, followed by an avidin-biotin-HRP conjugate (Vectastain, Vector Laboratories), according to the instructions of the supplier. Diaminobenzidine was the chromagen for the HRP reaction product, with glucose and glucose oxidase used to generate the hydrogen peroxide substrate (Itoh et al, 1979). Control sections in which the primary antiserum was omitted or absorbed with synthetic peptide or lectin (Peninsula Laboratories) completely eliminated reaction product.…”

Section: Methodsmentioning

confidence: 99%

Circadian rhythmicity restored by neural transplant. Immunocytochemical characterization of the graft and its integration with the host brain

Lehman¹,

Silver²,

Gladstone³

et al. 1987

J. Neurosci.

512

278

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It is well established that overt circadian rhythms are permanently disrupted following lesions of the hamster hypothalamic suprachiasmatic nucleus (SCN). In the present study, we show that implantations of brain grafts containing the fetal SCN reestablish circadian rhythms of locomotor activity in adult hamsters previously made arrhythmic by SCN lesions. The restoration of free-running rhythms in conditions of constant darkness is correlated with the presence in the graft of neuropeptides normally present in the SCN of unlesioned hamsters, including vasoactive intestinal polypeptide (VIP), neuropeptide Y (NPY), and vasopressin (VP). In several recipients, grafts were found to receive retinal input, and appeared to send efferents into the host brain. Not all functions of the SCN were reinstated by the graft: animals with restored locomotor rhythms did not show gonadal regression in the absence of light, and failed to synchronize (entrain) to light intensities to which SCN-intact animals responded.

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“…The peroxidase activity was visualized by means of the nickel-glucose oxidase technique. 19,20 Controls included replacement of primary and secondary antibodies with PBS, visualization of peroxidase only, and incubation of cells with normal rabbit serum (Sigma, Deisenhofen, Germany) in concentrations ranging from 0.1-0.01% rather than primary antiserum. Monoclonal or polyclonal antibodies were purchased as follows: KDR (Sigma), von Willebrand factor (Dakopatts), vascular endothelial (VE)-cadherin (Immunotech, Hamburg, Germany), CD31 (Pharmingen, Hamburg, Germany), and Tie2/ Tek (Santa Cruz Biotechnology, Santa Cruz, CA).…”

Section: Immunocytochemistrymentioning

confidence: 99%

Derivation of a new hematopoietic cell line with endothelial features from a patient with transformed myeloproliferative syndrome

Fiedler

Henke

Ergün

et al. 2000

Cancer

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Application of coupled oxidation reaction to electron microscopic demonstration of horseradish peroxidase: cobalt-glucose oxidase method

Cited by 526 publications

References 24 publications

Ascending Projections from the Area Postrema and the Nucleus of the Solitary Tract of Suncus Murinus

Ascending Projections from the Area Postrema and the Nucleus of the Solitary Tract of Suncus Murinus

Circadian rhythmicity restored by neural transplant. Immunocytochemical characterization of the graft and its integration with the host brain

Derivation of a new hematopoietic cell line with endothelial features from a patient with transformed myeloproliferative syndrome

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