Application of covalent affinity chromatography with thiol-disulphide interchange for determination of environmental exposure to heavy metals based on the quantitative isolation of Cd-thionein from human breast milk

Kabziński, A. K. M.

doi:10.1002/(sici)1099-0801(199807/08)12:4<217::aid-bmc738>3.0.co;2-2

Cited by 2 publications

(1 citation statement)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A second centrifugation step (30.0 min, 16,000 g, 4°C) was carried out to pellet the milk proteins. Milk protein standards of lactoferin, casein, albumin (Serva), lactoperoxidase, lgA, lgM, lgG and a-lactoalbumine (Sigma) were used for callibration of both LPGPC and HPGPC columns (Kabziński, 1997b(Kabziński, , 1998a(Kabziński, , 1998b(Kabziński, , 1998c(Kabziński, , 1999. Finally the concentrations of protein, thiol groups, Cd, Zn and Cu were measured (Table 2).…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of Cu-thionein from human fluids with application of solid-phase extraction on covalent affinity chromatography with thiol-disulphide interchange support

Kabziński

2000

Biomed. Chromatogr.

View full text Add to dashboard Cite

The aim of our investigation was to carry out quantitative isolation of Cu-thionein (Cu-Th) in human body fluids (urine, plasma and breast milk) in order to determine the level of exposure to heavy metals. In the experiment covalent affinity chromatography with thiol-disulphide interchange gel (CAC-TDI) was used as a solid phase extraction (SPE) support for preconcentration of Cu-thionein (Cu-Th) protein and Cu bonded with MT from water and human fluids samples. The present paper is a continuation of early experiments on the quantitation of Hg-thionein (Hg-Th), Cd-thionein (Cd-Th) and Zn-thionein (Zn-Th) in human body fluids such as urine, plasma and breast milk.

show abstract

Section: Introductionmentioning

confidence: 99%