Application of CRISPR/Cas9 Genome Editing System to Reduce the Pre- and Post-Harvest Yield Losses in Cereals

Krishna, Thumadath Palayullaparambil Ajeesh; Maharajan, Theivanayagam; Ceasar, S. Antony

doi:10.2174/18740707-v16-e2205190

Cited by 7 publications

(3 citation statements)

References 122 publications

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“…Nowadays, genome-editing tools are widely used to identify the roles of genes and transcription factors in response to biotic and abiotic stresses. Several articles have been published on the importance of genome-editing tools in cereals under biotic and abiotic stresses [110][111][112][113]. Genome-editing tools have not yet been implemented in any millet under any stress [114].…”

Section: Discussionmentioning

confidence: 99%

Identification of QTL Associated with Agro-Morphological and Phosphorus Content Traits in Finger Millet under Differential Phosphorus Supply via Linkage Mapping

et al. 2023

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Finger millet (Eleusine coracana (L.) Gaertn.) is one of the most important cereal crops for people in Asia and Africa. It supplies energy and nutrients for all people, particularly poor people in these continents. Phosphorus (P) is an essential macronutrient for plant growth and grain quality, so an adequate amount of P is required for healthy plant life. There is considerable evidence that P deficiency affects the growths and yields of all cereals, including finger millet. The present study aimed at identifying the quantitative trait loci (QTL) for various agro-morphological and P-content traits under low P (LP) and high P (HP) conditions through linkage mapping. Two cultivars, IE-2606 (low-P-susceptible) and PR-202 (low-P-tolerant) were used to develop 100 recombinant inbred lines (RILs). In total, 55 QTL, associated with various agro-morphological (primary root length (PRL), shoot fresh weight (SFW), root fresh weight (RFW), shoot dry weight (SDW), root dry weight (RDW), and root hair density (RHD)) and P-content traits (inorganic phosphate content in shoots (PiS), inorganic phosphate content in roots (PiR), and total P content in shoots (TPS)) were detected under LP conditions. Similarly, 37 QTL for various agro-morphological (PRL, RFW, SDW, and root hair length (RHL)) and P-content traits (PiS, PiR, TPS, and total P content in roots (TPR)) were detected under HP conditions. Biotic and abiotic stress-responsive candidate genes linked to a few QTL were also identified. The identified QTL associated with agro-morphological and P-content traits in finger millet under P deficiency could be used in breeding programs to develop finger millet with better P use efficiency (PUE). Furthermore, improving finger millet growth and yield under LP soil will help to reduce Pi application and eutrophication, which may help to improve the lives of poor farmers and maintain sustainable environments in Asia and African countries.

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Section: Discussionmentioning

confidence: 99%

Identification of QTL Associated with Agro-Morphological and Phosphorus Content Traits in Finger Millet under Differential Phosphorus Supply via Linkage Mapping

et al. 2023

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“…Once it reaches its target, Cas9 cuts the DNA, allowing researchers to insert, delete or modify genes as needed. This technology can be used to create wheat varieties that are resistant to pests by introducing genes that provide resistance (Krishna, Maharajan, & Ceasar, 2022).…”

Section: Crispr-cas9 and Rna Interference (Rnai)mentioning

confidence: 99%

A Mini Review on Insect Pests of Wheat and Their Management Strategies

2023

IJAB

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Insect pests pose a significant threat to wheat production globally, leading to substantial economic losses and reduced yields. This review paper provides an overview of the different types of insect pests that affect wheat, the damage they cause, and the methods used for their control. The major insect pests that infest wheat include aphids, Hessian fly, armyworms, and cereal leaf beetles. These pests cause damage by feeding on the leaves, stems, and developing grain, leading to reduced plant growth and ultimately reduced yield. Integrated Pest Management (IPM) is a widely accepted approach for sustainable management of insect pests in wheat. IPM involves a combination of biological, cultural, and chemical control methods to reduce pest populations and minimize pesticide use. The use of resistant wheat varieties, crop rotation, and sanitation practices are examples of cultural control methods that can reduce pest populations. Biological control methods involve the use of natural enemies such as predators, parasites, and pathogens to control insect pests. Chemical control methods are used as a last resort and involve the use of pesticides in a targeted and judicious manner. Biotechnology offers an alternative approach to controlling insect pests in wheat. The use of genetic engineering to develop insect-resistant wheat varieties has been successful in reducing insect pest damage. However, concerns about the potential ecological and social impacts of genetically modified organisms need to be addressed. In conclusion, sustainable management strategies that integrate biological, cultural, and chemical control methods are essential for effective control of insect pests in wheat production. The use of genetically modified organisms should be carefully evaluated to ensure that their use does not have adverse ecological and social impacts.

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“…Pre-harvest sprouting (PHS) and post-harvest food losses (PHFL) significantly undermine food security 1 – 3 . Seeds typically undergo a period of diminished germination capability known as dormancy, which is most pronounced at physiological maturity and diminishes thereafter 4 , 5 .…”

Section: Introductionmentioning

confidence: 99%

Wild rodents seed choice is relevant for sustainable agriculture

Peng,

Hu,

Dong

et al. 2024

Sci Rep

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Mitigating pre-harvest sprouting (PHS) and post-harvest food loss (PHFL) is essential for enhancing food securrity. To reduce food loss, the use of plant derived specialized metabolites can represent a good approach to develop a more eco-friendly agriculture. Here, we have discovered that soybean seeds hidden underground during winter by Tscherskia triton and Apodemus agrarius during winter possess a higher concentration of volatile organic compounds (VOCs) compared to those remaining exposed in fields. This selection by rodents suggests that among the identified volatiles, 3-FurAldehyde (Fur) and (E)-2-Heptenal (eHep) effectively inhibit the growth of plant pathogens such as Aspergillus flavus, Alternaria alternata, Fusarium solani and Pseudomonas syringae. Additionally, compounds such as Camphene (Cam), 3-FurAldehyde, and (E)-2-Heptenal, suppress the germination of seeds in crops including soybean, rice, maize, and wheat. Importantly, some of these VOCs also prevent rice seeds from pre-harvest sprouting. Consequently, our findings offer straightforward and practical approaches to seed protection and the reduction of PHS and PHFL, indicating potential new pathways for breeding, and reducing both PHS and pesticide usage in agriculture.

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Application of CRISPR/Cas9 Genome Editing System to Reduce the Pre- and Post-Harvest Yield Losses in Cereals

Cited by 7 publications

References 122 publications

Identification of QTL Associated with Agro-Morphological and Phosphorus Content Traits in Finger Millet under Differential Phosphorus Supply via Linkage Mapping

Identification of QTL Associated with Agro-Morphological and Phosphorus Content Traits in Finger Millet under Differential Phosphorus Supply via Linkage Mapping

A Mini Review on Insect Pests of Wheat and Their Management Strategies

Wild rodents seed choice is relevant for sustainable agriculture

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