Application of CRISPR/Cas9 in Crop Quality Improvement

Liu, Qier; Yang, Fan; Zhang, Jingjuan; Hang, Liu; Rahman, Shanjida; Islam, Shahidul; Ma, Wujun; She, Maoyun

doi:10.3390/ijms22084206

Cited by 107 publications

(50 citation statements)

References 143 publications

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“…These substitution lines have been widely used to integrate the D-genome into durum wheat cultivars. On the other hand, with the development of modern biotechnology, speedy and effective breeding of durum wheat can be achieved through molecular marker-assisted selection (MAS), genome sequencingassisted breeding, and target gene modification, such as RNA interference (RNAi) and CRISPR-Cas9, leading to many durum wheat lines with improved agronomic traits, protein quality, biotic resistance, abiotic tolerance, and reduced or eliminated triggering factors of wheat plant disorders [139][140][141][142][143][144]. /, unspecified; YPC, yellow pigment concentration; FHB, fusarium head blight; PM, powdery mildew; LR, leaf rust; SR, stem rust; YR, stripe rust; PHS, pre-harvest sprouting; LCYe, lycopene ε-cyclase; HYD2, β-carotene hydroxylase 2; HMW, high molecular weight; GW2, grain weight 2; WTAI, wheat α-amylase/trypsin inhibitors; CS, Chinese Spring.…”

Section: Strategies and Approachesmentioning

confidence: 99%

Improvement and Re-Evolution of Tetraploid Wheat for Global Environmental Challenge and Diversity Consumption Demand

Yang

Zhang

Liu

et al. 2022

IJMS

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Allotetraploid durum wheat is the second most widely cultivated wheat, following hexaploid bread wheat, and is one of the major protein and calorie sources of the human diet. However, durum wheat is encountered with a severe grain yield bottleneck due to the erosion of genetic diversity stemming from long-term domestication and especially modern breeding programs. The improvement of yield and grain quality of durum wheat is crucial when confronted with the increasing global population, changing climate environments, and the non-ignorable increasing incidence of wheat-related disorders. This review summarized the domestication and evolution process and discussed the durum wheat re-evolution attempts performed by global researchers using diploid einkorn, tetraploid emmer wheat, hexaploid wheat (particularly the D-subgenome), etc. In addition, the re-evolution of durum wheat would be promoted by the genetic enrichment process, which could diversify allelic combinations through enhancing chromosome recombination (pentaploid hybridization or pairing of homologous chromosomes gene Ph mutant line induced homoeologous recombination) and environmental adaptability via alien introgressive genes (wide cross or distant hybridization followed by embryo rescue), and modifying target genes or traits by molecular approaches, such as CRISPR/Cas9 or RNA interference (RNAi). A brief discussion of the future perspectives for exploring germplasm for the modern improvement and re-evolution of durum wheat is included.

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Section: Strategies and Approachesmentioning

confidence: 99%

Improvement and Re-Evolution of Tetraploid Wheat for Global Environmental Challenge and Diversity Consumption Demand

Yang

Zhang

Liu

et al. 2022

IJMS

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“…Genome editing systems, such as CRISPR/Cas, have been used for trait improvement of tubers, fibers, and cereal crops (Gao et al, 2017;Liu et al, 2021;Tussipkan and Manabayeva 2021). To our knowledge, there are few studies on using the CRISPR/Cas9 system for the enhancement of phytoremediation efficiency.…”

Section: Genome Editing Plants For Phytoremediationmentioning

confidence: 99%

Alfalfa (Medicago Sativa L.): Genotypic Diversity and Transgenic Alfalfa for Phytoremediation

Tussipkan

Manabayeva

2022

Front. Environ. Sci.

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Soil contamination caused by industrial and agricultural activities is an environmental problem that poses a serious risk to human health and the ecosystem. Persistent organic pollutants (POPs) are organic chemicals that persist in the environment for long periods because of their high resistance to photolytic, chemical, and biological degradation. Besides POPs, high concentrations of non-essential heavy metals and metalloids, such as arsenic, cadmium, and lead, are increasingly becoming a problem worldwide. Remediation strategies for organic and inorganic pollutants in the environment have received global attention. For organic or inorganic contaminants, phytoremediation is the strategy of choice because of a green technology that uses plants and solar energy to clean hyper-accumulated toxic pollutants from the environment. Some plant species have a high capacity to grow and survive in elevated levels of contaminants. With a long cultivation history and adaptability to a wide range of territories, alfalfa has not only widely been used for animal feed and a medicinal herb but is also an ideal natural resource and model plant for remediation of contaminated soils, offering a variety of elite characteristics. This review provides, firstly, abundant genomic information on the genetic diversity and population structure of alfalfa. Secondly, we focused on the transgenic alfalfa plants for enhanced phytoremediation of POPs, such as atrazine, polychlorinated biphenyl (PCB), and trichloroethylene (TCE), as well as phytoremediation of petroleum and heavy metals. Thirdly, the future perspective of enhancement of phytoremediation efficiency was discussed in depth. This review is intended to provide a comprehensive overview of the phytoremediation capabilities of transgenic alfalfa plants, presenting fundamental information for future research studies for enhancing phytoremediation efficiency.

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“…The coupling of gRNA and Cas9 nuclease will result in DSB upstream of the PAM motif [41]. In its application, genome editing in plants with CRISPR/Cas9 requires several stages, including identification of gRNAs, design and construction of transformation modules, delivery of construction modules in plant cells, plant regeneration, and screening of gene-edited plants [42].…”

Section: Crispr/cas9-based Genome Editingmentioning

confidence: 99%

Application of CRISPR/Cas9 technology to improve the important traits in coffee

Santoso¹,

Sisharmini

Syafaruddin

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Coffee is an essential estate crop that has a tremendous economic impact globally and is cultivated in many countries worldwide. However, due to the long process, the genetic improvement of coffee to develop new varieties through conventional breeding is slow and difficult. In addition, its production is constrained mainly by biotic and abiotic stresses. Therefore, developing superior coffee varieties with tolerance to abiotic and biotic stresses and improved quality is vital. The invention of genome editing technology has evolved the technologies in agriculture. CRISPR/Cas9 technology is a genome editing technique that more commonly applied for its simplicity, robustness, and high efficiency, compared to other techniques such as ZFN a nd TALEN. Furthermore, to develop new superior va rieties of coffee, the CRISPR/Cas9 system provides an opportunity to improve by adding desirable traits or removing unwanted characteristics. Therefore, genome editing techniques in coffee breeding can help to increase yield, resistant/tolerant of biotic a nd abiotic, reduce input costs, a nd improve product quality. This article discusses the latest innovations of the CRISPR/Cas9 technique applicable in agriculture and prospectively on genome editing to improve important characters in coffee.

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Application of CRISPR/Cas9 in Crop Quality Improvement

Cited by 107 publications

References 143 publications

Improvement and Re-Evolution of Tetraploid Wheat for Global Environmental Challenge and Diversity Consumption Demand

Improvement and Re-Evolution of Tetraploid Wheat for Global Environmental Challenge and Diversity Consumption Demand

Alfalfa (Medicago Sativa L.): Genotypic Diversity and Transgenic Alfalfa for Phytoremediation

Application of CRISPR/Cas9 technology to improve the important traits in coffee

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