Application of digital holographic microscopy to evaluate the dynamics of a single red blood cell influenced by low-power laser light

Kucharski, D.; Bartczak, Monika

doi:10.1016/j.optlastec.2021.107262

Cited by 5 publications

(4 citation statements)

References 43 publications

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“…where n is a positive integer. When n = 1, β 1 is the same to β in equation (9). The partial differential equation is solved to get a new level set function β n+1 from equation (11).…”

Section: Phase Processingmentioning

confidence: 99%

“…Digital holography (DH) has emerged as an important tool for achieving quantitative phase analyses [1][2][3][4][5][6][7][8][9][10][11]. Due to * Author to whom any correspondence should be addressed.…”

Section: Introductionmentioning

confidence: 99%

“…Since off-axis DH can separate the zero-order term and two cross-correlation terms in the Fourier spectrum from the recorded hologram, it allows phase processing using only one hologram in a single shot. Despite taking the cost of the space-bandwidth product, the applications of off-axis DHs have still been extended to various fields, such as surface topography [3,4], flow-field analysis [5,6], particle field measurement [7], and biomedicine field [8][9][10]. However, the reconstruction speed of traditional off-axis DH remains insufficient for practical applications.…”

Section: Introductionmentioning

confidence: 99%

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Fast and accurate phase processing in off-axis digital holography combining adaptive spatial filtering and an embedded GPU platform

Bai,

Li,

Sun

et al. 2024

Meas. Sci. Technol.

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Parallel-phase processing enables rapid phase extraction from off-axis digital holograms. To achieve fast and accurate results, the phase reconstruction processes were parallelized using improved filter algorithms and optimized programming strategies. First, an adaptive filtering method based on the Chan-Vese (CV) model which better suits parallelism was designed to extract the +1 term spectrum. We selected suitable computer unified device architecture (CUDA) libraries according to the characteristics of the key phase reconstruction steps. Acceleration technologies, such as virtual memory and shared memory, were used to improve the computational efficiency. Furthermore, we combined an improved 4f optical imaging system with an embedded graphic processing unit (GPU) platform to design a low-cost phase reconstruction system for off-axis digital holography. To verify the feasibility of our method, the reconstructed quality of the CV filtering method was estimated, and the run times of phase retrieval on the central processing unit (CPU) and embedded GPU were compared for off-axis holograms with different pixel sizes. Additionally, the dynamic fluctuation phase maps of water droplet evaporation were retrieved to demonstrate the real-time capability of the method.

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“…where n is a positive integer. When n = 1, β 1 is the same to β in equation (9). The partial differential equation is solved to get a new level set function β n+1 from equation (11).…”

Section: Phase Processingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fast and accurate phase processing in off-axis digital holography combining adaptive spatial filtering and an embedded GPU platform

Bai,

Li,

Sun

et al. 2024

Meas. Sci. Technol.

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“…Digital holography of particles is used to address various tasks both in laboratory and field conditions. These may include the study of particles of different nature in various media: plankton organisms [1][2][3][4][5][6][7][8], microplastic particles [9,10], gas bubbles in water [11][12][13], aerosol particles [14][15][16][17], defects in optical crystals [1], erythrocytes [18][19][20][21] and others. Particle holography typically uses an in-line hologram recording scheme [22][23][24][25][26], where the volume of the medium with particles is illuminated with a coherent radiation beam and a part of the radiation scattered on the particles represents an object wave, while the radiation that passed without scattering is a reference wave (Figure 1).…”

Section: Introductionmentioning

confidence: 99%

Geometric-Optical Model of Digital Holographic Particle Recording System and Features of Its Application

Dyomin,

Davydova,

Polovtsev

2024

Photonics

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The paper proposes an equivalent optical scheme of an in-line digital holographic system for particle recording and a mathematical model that establishes a one-to-one correspondence between the dimensional and spatial parameters of a digital holographic image of a particle and the imaged particle itself. The values of the model coefficients used to determine the real size and longitudinal coordinate of a particle according to its holographic image are found by calibration. The model was tested in field and laboratory conditions to calibrate a submersible digital holographic camera designed to study plankton in its habitat. It was shown that four calibration measurements are sufficient enough to determine the model coefficients, and the developed design of the submersible digital holographic camera makes it possible to perform these measurements during the recording of each hologram. In addition, this neither requires data on the refractive index of the medium with particles nor on the parameters of the optical elements of the scheme. The paper presents the results of marine experiments in the Kara Sea and the Laptev Sea, as well as in fresh water in laboratory conditions and in Lake Baikal. The error in measuring the particle size in seawater without the use of the model is 53.8%, while the error in determining their longitudinal coordinates is 79.3%. In fresh water, the same errors were 59% and 54.5%, respectively. The error in determining the position of a particle with the use of the designed mathematical model does not exceed 1.5%, and the error in determining the size is 4.8%. The model is sensitive to changes in the optical properties of the medium, so it is necessary to perform calibration in each water area, and one calibration is quite sufficient within the same water area. At the same time, the developed design of the submersible holographic camera allows, if necessary, calibration at each holographing of the medium volume with particles.

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A novel approach to using artificial intelligence in coordinate metrology including nano scale

et al. 2023

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Application of digital holographic microscopy to evaluate the dynamics of a single red blood cell influenced by low-power laser light

Cited by 5 publications

References 43 publications

Fast and accurate phase processing in off-axis digital holography combining adaptive spatial filtering and an embedded GPU platform

Fast and accurate phase processing in off-axis digital holography combining adaptive spatial filtering and an embedded GPU platform

Geometric-Optical Model of Digital Holographic Particle Recording System and Features of Its Application

A novel approach to using artificial intelligence in coordinate metrology including nano scale

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