Application of direct analysis in real time ionization–mass spectrometry (DART–MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud

Čajka, Tomáš; Danhelova, Hana; Zachariášová, Milena; Riddellová, Kateřina; Hajšlová, Jana

doi:10.1007/s11306-013-0495-z

Cited by 64 publications

(43 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3(a) According to the assigned results, various phosphatidylcholines (PC) and phosphatidylethanolamines (PE) were detected in the mass range of 400-800, while some triacylglycerols (TAGs) were observed in the mass range from 800 to 1000. These PC and PE were also determined in the pork [17] and the duck meat by HPLC-MS. [9] For those TAGs, they were identified in chicken meat by DART-MS. [12] As shown in Fig. 3, the intensity and amount of the mass spectra of the five meat samples show the difference and diversity.…”

Section: Discussionmentioning

confidence: 91%

“…Traditional high performance liquid chromatography-mass spectrometry (HPLC-MS) has also been applied to analyze the phospholipid molecular species in pork meat [8] and in duck meat. [9] Usually, a sample pretreatment is needed in the HPLC technique, and the analysis time is quite long (15-60 min).Besides 116 mixture of meats (beef, pork, horse, chicken and turkey), [10] 15 different meat samples [11] and chicken meat, [12] respectively, and exhibited a powerful ability for meat specification.For some ambient ion sources, which are based on the ESI technique, the sample is prone to getting disrupted because ESI calls for liquid samples with moderate ionic conductivity. Laser-ablation electrospray ionization (LAESI) was developed by Vertes et al in 2007.…”

mentioning

confidence: 99%

“…Besides 116 mixture of meats (beef, pork, horse, chicken and turkey), [10] 15 different meat samples [11] and chicken meat, [12] respectively, and exhibited a powerful ability for meat specification.…”

mentioning

confidence: 99%

“…Ambient mass spectrometry techniques generate ions in the open atmosphere and allow direct sample examination with minimal or no sample preparation requirement but remarkably high sample throughput. Desorption electrospray ionization mass spectrometry (DESI-MS), extractive electrospray ionization mass spectrometry (EESI-MS), and direct analysis in real time mass spectrometry (DART-MS) have been applied successfully to analyze a mixture of meats (beef, pork, horse, chicken and turkey), [10] 15 different meat samples [11] and chicken meat, [12] respectively, and exhibited a powerful ability for meat specification.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Rapid analysis and identification of meat species by laser‐ablation electrospray mass spectrometry (LAESI‐MS)

Zhou

Xia

Huang

et al. 2016

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

RATIONALE: Laser-ablation electrospray ionization mass spectrometry (LAESI-MS) was applied to analyze fresh meat species without sample pretreatment. The study demonstrates that the LAESI-MS technique is a promising, rapid and accurate method for meat identification using a protocol combining principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). METHODS: A focused IR-laser was used for meat sample ablation at a wavelength of 2940 nm. The ablated particulates were carried through a transfer PTFE tube using air as carrier gas, delivered to the electrospray plume and ionized. A TOF-MS was used to detect the ion signal. The raw mass spectra were analyzed using the PCA and PLS-DA protocol. RESULTS: Five fresh meat samples, chicken, duck, pork, beef and mutton, were identified by the developed LAESI-MS technique using the protocol combining PCA and PLS-DA. The discrimination accuracy of all meat species is 100%, and the score plot also shows good identifying ability. CONCLUSIONS: Five fresh meat samples were analyzed using the LAESI-MS technique. Each set of raw mass data was collected within 30 s and analyzed by the PCA and PLS-DA protocol. Eighteen, 19, 18, 17, and 15 markers for chicken, duck, pork, beef, and mutton, respectively, have been selected successfully for meat identification. The results demonstrate that LAESI-MS is a new reliable and rapid method for meat identification. Copyright © 2016 John Wiley & Sons, Ltd.The consumption of meat keeps constantly increasing in the world. As reported by Sans et al., meat consumption rose worldwide from 23.1 kg per person per year in 1961 to 42.20 kg per person per year in 2011. [1] As a result, the safety of meat has drawn extensive attention by society. Consumers are increasingly concerned about the quality of the meat they eat due to the many meat scandals which have occurred recently. [2] In order to detect fraudulent species in meat, many techniques have been developed. Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) methods are routinely used for the specification of meat. [3] The ELISA method can be used as a fast qualitative or quantitative technique. One hundred meat samples and meat products have been analyzed for species determination by the ELISA method. The results showed that 22.0% of the samples were not in compliance with Turkish Food Codex, violating consumer rights and presenting a potential public health risk. [4] However, using an ELISA it is difficult to analyze different species in a single run, and also is quite expensive. [3] PCR methods are widely applied to identify meat species in routine laboratories. Only 0.1% of raw pork in poultry was detected by the PCR method. [5] However, the PCR method is not widely implemented in food control programs due to complex operation or financial reasons.Recently, mass spectrometry has become an important tool for the identification of meat due to its intrinsic high sensitivity and high specificity. Matrix-assisted laser desorption ionizat...

show abstract

Section: Discussionmentioning

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Rapid analysis and identification of meat species by laser‐ablation electrospray mass spectrometry (LAESI‐MS)

Zhou

Xia

Huang

et al. 2016

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…In the same years, a new analytical approach based on the coupling between Direct Analysis in Real Time (DART) ionization source and different types of mass spectrometers was proposed for authentication and traceability of several food items such as meat, beer, and olive oil [83][84][85][86]. DART coupled to high-resolution mass spectrometry (HRMS) was also applied for the discrimination among different types of milk obtained from various farm animal species (cow, goat, and sheep), the distinction between cows' milk produced in conventional and organic farming, and the detection of vegetable oil added to a milk-based product (soft cheese) [87].…”

Section: Analytical Methods For Food Authenticity and Traceabilitymentioning

confidence: 99%

Overview on Untargeted Methods to Combat Food Frauds: A Focus on Fishery Products

Fiorino

Garino

Arlorio

et al. 2018

Journal of Food Quality

View full text Add to dashboard Cite

Authenticity and traceability of food products are of primary importance at all levels of the production process, from raw materials to finished products. Authentication is also a key aspect for accurate labeling of food, which is required to help consumers in selecting appropriate types of food products. With the aim of guaranteeing the authenticity of foods, various methodological approaches have been devised over the past years, mainly based on either targeted or untargeted analyses. In this review, a brief overview of current analytical methods tailored to authenticity studies, with special regard to fishery products, is provided. Focus is placed on untargeted methods that are attracting the interest of the analytical community thanks to their rapidity and high throughput; such methods enable a fast collection of "fingerprinting signals" referred to each authentic food, subsequently stored into large database for the construction of specific information repositories. In the present case, methods capable of detecting fish adulteration/substitution and involving sensory, physicochemical, DNA-based, chromatographic, and spectroscopic measurements, combined with chemometric tools, are illustrated and commented on.

show abstract

Biological Samples in Environmental Analysis: Preparation and Cleanup

Lawton

Santee

Bruno

et al. 2016

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

The characterization of biological systems on a molecular level has led to sweeping developments in industries including pharmaceuticals, consumer product manufacturing, and environmental monitoring. The integrity of the sample preparation process is critical for development and discovery efforts in these fields. The art of biological sample preparation is in a perpetual state of evolution where boundaries and limitations reflect the sophistication of contemporary technology. Historical methods required laborious multistep procedures that resulted in analytical measurements with limited throughput and poor detection limits due to analyte losses. Present efforts in the scientific community are moving toward automated methods that require minimal sample volume and minimal sample cleanup. This change in the approach is due to the increasing selectivity of analytical systems that allow analyte separation and purification to be performed online with a variety of detectors. Trends of this nature are consistent with the ‘green chemistry’ efforts, where less solvent consumption provides a more environmental friendly (and cost‐effective) platform for the next stage of development in this field. This article revisits historical approaches and describes current trends in the preparation of biological samples.

show abstract

Application of direct analysis in real time ionization–mass spectrometry (DART–MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud

Cited by 64 publications

References 38 publications

Rapid analysis and identification of meat species by laser‐ablation electrospray mass spectrometry (LAESI‐MS)

Rapid analysis and identification of meat species by laser‐ablation electrospray mass spectrometry (LAESI‐MS)

Overview on Untargeted Methods to Combat Food Frauds: A Focus on Fishery Products

Biological Samples in Environmental Analysis: Preparation and Cleanup

Contact Info

Product

Resources

About