Application of DNA microarray technology to influenza A/Vietnam/1194/2004 (H5N1) vaccine safety evaluation

Strategic Applications of Gene Expression: From Drug Discovery/Development to Bedside

“…Several reports have been published in the past few years using gene expression profiling technology to evaluate vaccine safety. In general, the gene expression results were consistent with the degree of toxic effects observed in more traditional assays, such as the abnormal toxicity test and the leukopenic toxicity test (72)(73)(74). More recently, the systems vaccinology approach describes using the genome-wide gene expression underlying the host responses to vaccination (75)(76)(77)(78).…”

The Widely Used Diagnostics “DNA Microarray”-A Review

“…They could not find any cross-hybridization reaction with other viruses, indicating that the microarray is specific for influenza A viruses and can be undoubtedly be useful for identifying novel influenza A virus subtypes. Mizukami and co-researchers [36] proposed a DNA microarray analysis in the quality control of pandemic and endemic influenza virus whole-virion influenza vaccine, whole virion-particle vaccine and sub-virion vaccine (HA vaccine) and concluded that DNA microarray technology is an informative, rapid and highly sensitive method to evaluate the quality of influenza vaccines.…”

Na+-Ca2+ Exchanger Expression and Its Modulation

“…[24][25][26] The aliquots of 1.5-mg RNA were subjected to first-strand cDNA synthesis in the presence of Cyanine-5 dioxyuridine triphosphate (dUTP) (PerkinElmer, Boston, MA, U.S.A.) for samples obtained from the rat tissues or Cyanine-3 dUTP (PerkinElmer) for a common reference RNA (MicroDiagnostic) in a reaction mixture derived from a labeling and hybridization kit (MicroDiagnostic). The red fluorescence-labeled cDNA of the samples and the green fluorescence-labeled common reference RNA were equally mixed and hybridized to a microarray printed with the 80-mer polynucleotides using the labeling and hybridization kit.…”