Objective: To explore the clinical application value of humanistic nursing care in the treatment of hematologic neoplasm inpatients. Methods: Fifty-two patients with hematologic neoplasms admitted to a hospital from May 2019 to February 2022 were selected as the research subjects. According to a random number table, they were divided into two groups: the control group (n = 25, routine clinical nursing) and the observation group (n = 27, humanistic nursing care). The negative emotion score, nursing satisfaction, and sleep quality were compared between the two groups under different nursing modes. Results: The SAS and SDS scores before and after nursing were compared between the two groups. There was no significant difference between the two groups before nursing (p > 0.05). However, the SDS and SAS scores in the two groups after nursing were lower than those before nursing, in which the observation group was slightly lower than the control group, and the difference was statistically significant (p < 0.001). In terms of nursing satisfaction, it was as high as 96.29% in the observation group, whereas in the control group, the satisfaction rate was only 72.00%; the PSQI scores were compared between the two groups before and after nursing, and there was no significant difference between the two groups before nursing (p > 0.05). However, the PSQI scores and total score of the observation group after nursing were lower than those of the control group (p < 0.001). Conclusion: In the clinical treatment of patients with hematologic neoplasms, the implementation of humanistic nursing care can significantly improve patients’ anxiety, depression, other negative emotions, sleep quality, and nursing satisfaction, all of which have significance in promoting the prognosis of patients and improving their quality of life.