Application of dried blood spots combined with HPLC-MS/MS for the quantification of acetaminophen in toxicokinetic studies

Barfield, Matt; Spooner, Neil; Lad, Rakesh; Parry, Simon; Fowles, Susan

doi:10.1016/j.jchromb.2008.05.025

Cited by 252 publications

(177 citation statements)

References 15 publications

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“…According to the manufacturer, both cards are chemically treated with proprietary reagents that, upon contact, lyse cells, denature proteins and prevent the growth of bacteria and other microorganisms. Recently, both cards have been employed in combination with LC-MS/MS for the quantitative analysis of small drug molecules and their metabolites in preclinical and clinical studies (Barfi eld et al, 2008;Liang et al, 2009;Spooner et al, 2009). …”

Section: Dbs Paper/cardmentioning

confidence: 99%

“…The combined advantage of the above allows for a signifi cant simplifi cation of blood sample collection and handling for newborns, infants, small animals and other special patient populations for various studies. Currently, more than 95% of newborns are screened for inherited metabolic disorders using DBS in the USA (Chace et al, 2002;Wilcken and Wiley, 2008) and the application of DBS has been extended to many other fi elds, including therapeutic drug monitoring (Edelbroek et al, 2009), toxicokinetics (Barfi eld et al, 2008) and pharmacokinetics (Spooner et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Li¹,

Tse²

2009

Biomedical Chromatography

542

428

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The collection of whole blood samples on paper, known as dried blood spot (DBS), dates back to the early 1960s in newborn screening for inherited metabolic disorders. DBS off ers a number of advantages over conventional blood collection. As a less invasive sampling method, DBS off ers simpler sample collection and storage and easier transfer, with reduced infection risk of various pathogens, and requires a smaller blood volume. To date, DBS-LC-MS/MS has emerged as an important method for quantitative analysis of small molecules. Despite the increasing popularity of DBS-LC-MS/MS, the method has its limitations in assay sensitivity due to the small sample size. Sample quality is often a concern. Systematic assessment on the potential impact of various blood sample properties on accurate quantifi cation of analyte of interest is necessary. Whereas most analytes may be stable on DBS, unstable compounds present another challenge for DBS as enzyme inhibitors cannot be conveniently mixed during sample collection. Improvements on the chemistry of DBS card are desirable. In addition to capturing many representative DBS-LS-MS/MS applications, this review highlights some important aspects of developing and validating a rugged DBS-LC-MS/MS method for quantitative analysis of small molecules along with DBS sample collection, processing and storage.

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Section: Dbs Paper/cardmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Li¹,

Tse²

2009

Biomedical Chromatography

542

428

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“…The availability of highly sensitive analytical techniques, mainly liquid chromatographytandem mass spectrometry (LC-MS/MS), resulted in an exponential increase in the interest in DBS for applications in other fields, including therapeutic drug monitoring [16,17], toxicology [18,19] and toxico-and pharmacokinetic studies in (pre-)clinical phases of drug development [20][21][22]. In general, DBS are prepared by applying a drop of capillary blood, obtained by a finger or heel prick, on a dedicated filter paper card.…”

Section: Dried Blood Spotsmentioning

confidence: 99%

Alternative Sampling Strategies for Cytochrome P450 Phenotyping

2015

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Interindividual variability in the expression and function of drug metabolizing cytochrome P (CYP) 450 enzymes, determined by a combination of genetic, non-genetic and environmental parameters, is a major source of variable drug response. Phenotyping by administration of a selective enzyme substrate, followed by the determination of a specific phenotyping metric, is an appropriate approach to assess the in vivo activity of CYP450 enzymes, as it takes into account all influencing factors. A phenotyping protocol should be as simple and convenient as possible. Typically, phenotyping metrics are determined in traditional matrices, such as blood, plasma or urine. Several sampling strategies have been proposed as an alternative for these traditional sampling techniques.In this review, we provide a comprehensive overview of available methods using dried blood spots, hair, oral fluid, exhaled breath and sweat for in vivo CYP450 phenotyping. We discuss the relation between phenotyping metrics measured in these samples and those in conventional matrices, along with the advantages and limitations of the alternative sampling techniques. Reliable phenotyping procedures for several clinically relevant CYP450 enzymes, including CYP1A2, CYP2C19 and CYP2D6, are currently available for oral fluid, breath or dried blood spots, while additional studies are needed for other CYP450 isoforms, such as CYP3A4. The role of hair analysis for this purpose remains to be established. Being non-or minimally invasive, these sampling strategies provide convenient and patient-friendly alternatives for classical phenotyping procedures, which may contribute to the implementation of CYP450 phenotyping in clinical practice. 4 Key Points Phenotyping by administration of a selective probe drug, followed by determination of a specific phenotyping metric, allows to assess the in vivo enzyme activity of CYP450 enzymes, taking into account genetic, non-genetic and environmental influencing factors. In addition to classical sampling techniques, such as blood or urine collection, reliable phenotyping procedures for several clinically relevant CYP450 enzymes are currently available for oral fluid, breath and dried blood spots.

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“…Quantification of drugs using the dried plasma spot sampling technique has been described for daptomycin [10], valproic acid and gabapentin [11,12], linezolid [13], paroxetine [14], acetaminophen [15], guanfacine [16], iothalamate [17], vigabatrin [18], triazoles [19], and antiepileptic drugs [20], as well as anti-HIV drugs [21].…”

Section: Introductionmentioning

confidence: 99%

A validated method for the quantification of fosfomycin on dried plasma spots by HPLC–MS/MS: Application to a pilot pharmacokinetic study in humans

Parker

Lipman

Dimοpoulos

et al. 2015

Journal of Pharmaceutical and Biomedical Analysis

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Application of dried blood spots combined with HPLC-MS/MS for the quantification of acetaminophen in toxicokinetic studies

Cited by 252 publications

References 15 publications

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Dried blood spot sampling in combination with LC‐MS/MS for quantitative analysis of small molecules

Alternative Sampling Strategies for Cytochrome P450 Phenotyping

A validated method for the quantification of fosfomycin on dried plasma spots by HPLC–MS/MS: Application to a pilot pharmacokinetic study in humans

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