Application of electrochemically produced aluminum hydroxide gel for prepurification of recombinant synthetic green fluorescent protein produced in tobacco leaves

Abstract

The use of recombinant proteins has increased greatly in recent years, as also have increased the number of techniques and materials used for their production and purification. Among the different types of bioreactors being studied, there is a general consensus among scientists that production in green plant tissues such as leaves is more feasible. However, the presence of chlorophyll and phenolic compounds in plant extracts, which can precipitate and denature the proteins besides damaging separation membranes and gels, makes this technology impracticable on a commercial scale. In the present work, the adsorption to electrochemically produced aluminum hydroxide gel was applied as a prepurification step for recombinant synthetic green fluorescent protein (sGFP), also referred to as enhanced green fluorescent protein, produced in Nicotiana benthamiana leaves. Removal efficiencies of 99.7% of chlorophyll, 88.5% of phenolic compounds, and 38.5% of native proteins from the N. benthamiana extracts were achieved without removing sGFP from the extracts. As electrochemical preparation of aluminum hydroxide gel is a cost-effective technique, its use can substantially contribute to the development of future production platforms for recombinant proteins produced in green plant tissues of pharmaceutical and industrial interest.

Single Source Precursor Chemical Vapor Decomposition Method to Fabricate Stable, Bright Emissive Aluminum Hydroxide Phosphors for UV‐Pumped White Light‐Emitting Devices

“…Aluminum hydroxide, a very common, low‐cost intermediate compound in aluminum and aluminum oxide industry, exhibits different stable structures (Al(OH) 3 , AlOOH, 5Al 2 O 3 3H 2 O) and has been applied in plenty of fields including flame retardants, catalysis, waste treatment, medical and petrochemical industries . In recent years, different kinds of nanosized aluminum hydroxides such as γ‐AlOOH nanotubes, nanorods, nanoplates, nanofibers, nanobelts, nanoparticles, and nanowires, as well as Al(OH) 3 nanocrystals (NCs), have been reported.…”