Application of Electromodulated Fluorescence to the Study of the Dynamics of Single-Strand Oligonucleotides at Modified Gold Surfaces

Wang, Lili; Silin, V. P.; Gaigalas, Adolfas K.; Xia, Jiliang; Gebeyehu, Gulilat

doi:10.1006/jcis.2001.8191

Cited by 4 publications

(3 citation statements)

References 27 publications

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“…The corresponding fluorescence measurements show a small increase in the fluorescence at the negative potential limit. This potential-dependent fluorescence was used in the study of physically adsorbed surfactants on gold electrodes ,, as well as by Gaigalas in the study of the dynamics of immobilized fluorophores , and fluorescein-labeled single-strand oligonucleotides immobilized onto gold . The changes in fluorescence are due to a slight increase in the distance between the fluorophore and the electrode surface.…”

Section: Resultsmentioning

confidence: 99%

“…This potentialdependent fluorescence was used in the study of physically adsorbed surfactants on gold electrodes 63,70,71 as well as by Gaigalas in the study of the dynamics of immobilized fluorophores 72,73 and fluorescein-labeled single-strand oligonucleotides immobilized onto gold. 74 The changes in fluorescence are due to a slight increase in the distance between the fluorophore and the electrode surface. The increase in interfacial capacitance suggests a partial desorption of some thiol, with the desorbed molecules moving away from the interface.…”

Section: Electrochemical and Epi-fluorescence Investigationsmentioning

confidence: 99%

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Selective Reductive Desorption of a SAM-Coated Gold Electrode Revealed Using Fluorescence Microscopy

et al. 2004

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The reductive desorption of a self-assembled monolayer (SAM) of a fluorescent thiol molecule (BodipyC10SH) from Au was characterized using electrochemistry and epi-fluorescence microscopy. Molecular luminescence is quenched near a metal surface, so fluorescence was only observed for molecules reductively desorbed and then separated from the electrode surface. Fluorescence imaging showed that reductive desorption was selective, with desorption occurring from different regions of the Au electrode depending on the extent of the negative potential excursion. When desorbed, the molecules were sufficiently mobile, diffusing away from the electrode surface, thereby preventing oxidative readsorption. At sufficiently negative desorption potentials, all of the thiol was desorbed from the electrode surface, resulting in fluorescence at the air/solution interface. The selective removal of the thiol monolayer from distinct regions was correlated to features on the electrode surface and was explained through potential-dependent interfacial energies. This in situ electrofluorescence microscopy technique may be useful in sensor development.

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Section: Resultsmentioning

confidence: 99%

Section: Electrochemical and Epi-fluorescence Investigationsmentioning

confidence: 99%

Selective Reductive Desorption of a SAM-Coated Gold Electrode Revealed Using Fluorescence Microscopy

et al. 2004

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“…This method is therefore useful for investigation of the desorbed surfactants as they become separated from the electrode surface. Recently, electromodulated fluorescence has been used to examine the changes that occur on gold electrodes modified with thiol SAMs as a function of potential [39,[113][114][115]. Fig.…”

Section: Fluorescence Microscopymentioning

confidence: 99%

Electrochemical and spectroelectrochemical characterization of lipid organization in an electric field

Bizzotto¹,

Yang²,

Shepherd³

et al. 2004

Journal of Electroanalytical Chemistry

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Epi‐Fluorescence Microscopy Studies of Potential Controlled Changes in Adsorbed Thin Organic Films at Electrode Surfaces

Bizzotto¹,

Shepherd²

2006

Advances in Electrochemical Sciences and Engineering

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Application of Electromodulated Fluorescence to the Study of the Dynamics of Single-Strand Oligonucleotides at Modified Gold Surfaces

Cited by 4 publications

References 27 publications

Selective Reductive Desorption of a SAM-Coated Gold Electrode Revealed Using Fluorescence Microscopy

Selective Reductive Desorption of a SAM-Coated Gold Electrode Revealed Using Fluorescence Microscopy

Electrochemical and spectroelectrochemical characterization of lipid organization in an electric field

Epi‐Fluorescence Microscopy Studies of Potential Controlled Changes in Adsorbed Thin Organic Films at Electrode Surfaces

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