Application of electrospray mass spectrometry in biology

Pitt, Andrew R.

doi:10.1039/a815059y

Cited by 15 publications

(11 citation statements)

References 198 publications

(169 reference statements)

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“…A description of the instrumentation has been documented elsewhere. [16][17][18][19][20][21][22][23][24] The mass spectrometer was coupled to a Shimadzu LC-10AD pump and an SCL-10A system controller (Shimadzu, Columbia, Maryland, USA). Calibration was performed on both the first quadrupole (Q1) and the third quadrupole (Q3) using a solution of polypropylene glycol (PPG) in 3 mM ammonium acetate.…”

Section: Methodsmentioning

confidence: 99%

Mass spectrometric characterization of polyhedral oligosilsesquioxanes and heterosilsesquioxanes

Bakhtiar

Feher

1999

Rapid Commun. Mass Spectrom.

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The term 'silsesquioxane' refers to structures with an even number of silicon atoms (i.e. [RSiO 3/2 ] n with n = even integer) and extended network solids (i.e. [RSiO 3/2 ]) which can achieve the idealized empirical formula for silsesquioxanes, (RSiO 3/2 ) ? . In order to avoid confusion, it is often useful to distinguish between 'fully condensed' and 'incompletely condensed' silisesquioxane frameworks. A fully condensed silsesquioxane has an empirical formula of [RSiO 3/2 ] n , whereas an incompletely condensed silsesquioxane possesses reactive Si-OH groups. A host of heterosilsesquioxanes including main-group, transitionmetal and lanthanide metallasilsesquioxanes are also available via functionalization of incompletely condensed silsesquioxanes. Recent applications include soluble models for silica-supported catalysts, and precursors to hybrid inorganic/organic materials. 1-14 For these reasons metallasilsesquioxanes generated from the coordination of incompletely condensed silsesquioxanes to high-valent transition metals have emerged as useful solution-state models for silica-supported catalysts.15 A wide range of silsesquioxanes can be prepared via the hydrolytic condensation reactions of trifunctional organosilicon monomers, as presented in Scheme 1.Herein, using mass spectrometry, we present evidence on the generation and characterization of a selected group of silsesquioxanes in neat hexane or n-hexane/ethanol (i.e. 90:10, v/v). The results in this study indicate that atmospheric pressure chemical ionization (APCI) and turbo ionspray (TISP) mass spectrometry (MS) could be useful Scheme 1.

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Section: Methodsmentioning

confidence: 99%

Mass spectrometric characterization of polyhedral oligosilsesquioxanes and heterosilsesquioxanes

Bakhtiar

Feher

1999

Rapid Commun. Mass Spectrom.

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“…After the human genome was decoded, the exploration of the proteome has become the focus of interest [4]. Electrospray mass spectrometry techniques in particular are suitable for on-line coupling with liquid chromatography systems and therefore allow rapid and direct identification of peptides [5].…”

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confidence: 99%

Investigation of the influence of charge derivatization on the fragmentation of multiply protonated peptides

Sonsmann¹,

Römer²,

Schomburg³

2002

J. Am. Soc. Mass Spectrom.

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The fragmentation of the multiply charged peptides b-chain of bovine insulin and glucagon have been investigated under low energy collision induced dissociation (CID) conditions using an electrospray ion trap mass spectrometer. The influence of charge state, specific amino acids such as aspartate or proline, the location of basic sites, and the derivatization on the fragmentation behavior has been the focus of interest. As a basis for understanding the fragmentation process, the concept of the mobile proton was applied. A set of different derivatives was used to manipulate the sites of protonation of the peptides in order to control and improve the fragmentation behavior. These results can be applied for de novo sequencing, although the sequence-specific fragmentation processes have significant influence on the dissociation behavior of the peptides. (J Am Soc Mass Spectrom 2002, 13, 47-58)

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“…In CH 3 CN solutions, neutral rhenium complexes such as (Ind)Re(CO) 3 (Ind = indenyl) and (Cp)Re(CO) 3 (Cp = cyclopentadienyl) gave rise to [Re(CO) 3 (CH 3 CN) 3 ] ions. 57 Interestingly, these complexes did not react with CH 3 CN under regular laboratory conditions.…”

Section: Organometallic and Inorganic Chemistrymentioning

confidence: 99%

“…Ferrocene (Fc)-based phosphine ligands, including several ferrocenylmethyl complexes, FcCH 2 X (X = PO 3 Ph 2 , NMe 2 , NMe 3 and OH) were investigated by Henderson et al 40 using a single-stage quadrupole ESI-MS unit. Collision-induced dissociation (CID) of the amine and hydroxyl derivatives afforded the relatively stable carbocation FcCH 2 as the predominant fragmentation product.…”

Section: Organometallic and Inorganic Chemistrymentioning

confidence: 99%

“…57 Interestingly, these complexes did not react with CH 3 CN under regular laboratory conditions. Neutral rhenium bipyridine complexes have also been analyzed by using ionization agents such as NaNO 3 . 61 Presumably, the interaction of Na with the bipyridine moiety (in neat methanol) resulted in signals corresponding to [ exhibited reactivity with methanol during the ESI process while they were inert in acetonitrile solutions.…”

Section: Organometallic and Inorganic Chemistrymentioning

confidence: 99%

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Unravelling seemingly complex chemistry of reactions using electrospray ionization mass spectrometry

Bakhtiar

Hop

1999

J. Phys. Org. Chem.

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Since its discovery, electrospray ionization mass spectrometry (ESI-MS) has provided valuable information on structurally specific biomolecular interactions, including DNA-drug, enzyme-inhibitor, DNAprotein and protein-protein interactions. In addition, this technique has emerged as a powerful tool in probing the structural modification of large molecules. However, ESI-MS has been underutilized in analysis and mechanistic studies involving small molecules. Here, a brief overview is presented to familiarize non-specialists with ESI-MS applications in analysis and mechanistic studies of small molecules. For more advanced applications (e.g. biopolymers) or detailed analytical descriptions of the ionization processes and the equipment, readers are referred to a number of excellent reviews and books.

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Application of electrospray mass spectrometry in biology

Cited by 15 publications

References 198 publications

Mass spectrometric characterization of polyhedral oligosilsesquioxanes and heterosilsesquioxanes

Mass spectrometric characterization of polyhedral oligosilsesquioxanes and heterosilsesquioxanes

Investigation of the influence of charge derivatization on the fragmentation of multiply protonated peptides

Unravelling seemingly complex chemistry of reactions using electrospray ionization mass spectrometry

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