Application of Elements of Quality by Design to Development and Optimization of HPLC Method for Fingolimod

Ansari, Siddique Akber; Deshpande, Mrinmayee; Sangshetti, Jaiprakash N.; Ahmed, Sarfaraz; Ansari, Irfan Aamer

doi:10.9734/jpri/2021/v33i46a32872

Cited by 2 publications

(3 citation statements)

References 6 publications

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“…In light of our experimentation, it was discovered that the retention times for the formulation in question were 7.53 and 7.52 min (Supplementary Figure S1B,C). The results confirmed that this method was validated with respect to every aspect of the experiment, including stability, accuracy, precision, and linearity [19].…”

Section: Evaluation Of Quantificationsupporting

confidence: 66%

Assessing the In Vitro and In Vivo Performance of L-Carnitine-Loaded Nanoparticles in Combating Obesity

Uner,

Ergin,

Ansari

et al. 2023

Molecules

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Addressing obesity is a critical health concern of the century, necessitating urgent attention. L-carnitine (LC), an essential water-soluble compound, plays a pivotal role in lipid breakdown via β-oxidation and facilitates the transport of long-chain fatty acids across mitochondrial membranes. However, LC’s high hydrophilicity poses challenges to its diffusion through bilayers, resulting in limited bioavailability, a short half-life, and a lack of storage within the body, mandating frequent dosing. In our research, we developed LC-loaded nanoparticle lipid carriers (LC-NLCs) using economically viable and tissue-localized nanostructured lipid carriers (NLCs) to address these limitations. Employing the central composite design model, we optimized the formulation, employing the high-pressure homogenization (HPH) method and incorporating Poloxamer® 407 (surfactant), Compritol® 888 ATO (solid lipid), and oleic acid (liquid oil). A comprehensive assessment of nanoparticle physical attributes was performed, and an open-field test (OFT) was conducted on rats. We employed immunofluorescence assays targeting CRP and PPAR-γ, along with an in vivo rat study utilizing an isolated fat cell line to assess adipogenesis. The optimal formulation, with an average size of 76.4 ± 3.4 nm, was selected due to its significant efficacy in activating the PPAR-γ pathway. Our findings from the OFT revealed noteworthy impacts of LC-NLC formulations (0.1 mg/mL and 0.2 mg/mL) on adipocyte cells, surpassing regular L-carnitine formulations’ effects (0.1 mg/mL and 0.2 mg/mL) by 169.26% and 156.63%, respectively (p < 0.05).

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Section: Evaluation Of Quantificationsupporting

confidence: 66%

Assessing the In Vitro and In Vivo Performance of L-Carnitine-Loaded Nanoparticles in Combating Obesity

Uner,

Ergin,

Ansari

et al. 2023

Molecules

Self Cite

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“…The mobile phase was composed of 90% acetonitrile and 10% 20 mM ammonium formate buffer (pH 5.8). [45] The loading content of FTY720 in nanoparticles was calculated as loading…”

Section: Methodsmentioning

confidence: 99%

“…The mobile phase was composed of 90% acetonitrile and 10% 20 mM ammonium formate buffer (pH 5.8). [ 45 ]…”

Section: Methodsmentioning

confidence: 99%

Lymph‐Node‐Targeted Drug Delivery for Effective Immunomodulation to Prolong the Long‐Term Survival After Heart Transplantation

et al. 2022

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The chronic rejection responses and side effects of the systematic administration of immunosuppressants are the main obstacles to heart allograft and patient survival. The development of xenotransplantation also urgently requires more efficient immune regulation strategies. Herein, it is demonstrated that lymph‐node (LN)‐targeted drug delivery can realize LN‐specific immunomodulation with attenuated immune suppression on distant peripheral immune organs to effectively prolong long‐term survival after heart transplantation in a chronic murine heart transplantation model. A chemokine C‐C motif ligand 21 (CCL21) specific aptamer for LN targeting is decorated onto the surface of the hybrid nanoparticular delivery vector mainly composed of CaCO3/CaP/heparin. The targeting delivery system can dramatically enhance accumulation of the loaded immunosuppressant, fingolimod hydrochloride (FTY720), in draining lymph nodes (dLNs) for inducing powerful immune suppression. By promoting the generation of endogenous regulatory T cells (Tregs) and decreasing the proportion of effector T cells (Teffs) in dLNs after heart transplantation, the LN‐targeting strategy can effectively regulate local immune responses instead of systemic immunity, which reduces the incidence of long‐term complications. This study provides an efficient strategy to improve the survival rate after organ transplantation by precise and localized immunoregulation with minimized side effects of immunosuppression.

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Application of Elements of Quality by Design to Development and Optimization of HPLC Method for Fingolimod

Cited by 2 publications

References 6 publications

Assessing the In Vitro and In Vivo Performance of L-Carnitine-Loaded Nanoparticles in Combating Obesity

Assessing the In Vitro and In Vivo Performance of L-Carnitine-Loaded Nanoparticles in Combating Obesity

Lymph‐Node‐Targeted Drug Delivery for Effective Immunomodulation to Prolong the Long‐Term Survival After Heart Transplantation

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