Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic <i>Bacillus licheniformis</i> NCIMB 8059

Białkowska, Aneta; Gromek, Ewa; Krysiak, Joanna; Sikora, Barbara; Kalinowska, Halina; Jędrzejczak-Krzepkowska, Marzena; Kubik, C.; Lang, Siegmund; Schütt, Fokko; Turkiewicz, Marianna

doi:10.1007/s10295-015-1697-3

Cited by 28 publications

(12 citation statements)

References 28 publications

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“…Jiang et al cultivated Paenibacillus polymyxa CICC 10010, in an enzymatic hydrolysate of microcrystalline cellulose with glucose concentrations of approximately 100 g/L, cultivated at 30°C and 200 rpm, obtaining a maximum 2,3‐BD production of 40 g/L and productivity of 0.70 g/(L/h). Białkowska et al used the enzymatic hydrolysate of apple pomace (approximately 43 g/L of glucose) to cultivate Bacillus licheniformis NCIMB 8059, under conditions of 37°C and 130 rpm in shake flask batch fermentations, obtaining 2,3‐BD yields of 0.36 g/g and productivity of 0.66 g/(L/h). When the authors used the same medium, but running a fed‐batch cultivation, the production was up to 113 g/L of 2.3‐BD.…”

Section: Resultsmentioning

confidence: 99%

Production of 2,3‐butanediol byKlebsiella pneumoniaeBLh‐1 andPantoea agglomeransBL1 cultivated in acid and enzymatic hydrolysates of soybean hull

Cortivo

Machado

Hickert

et al. 2019

Biotechnology Progress

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We investigated the production of 2,3-butanediol by two enterobacteria isolated from an environmental consortium, Klebsiella pneumoniae BLh-1 and Pantoea agglomerans BL1, in a bioprocess using acid and enzymatic hydrolysates of soybean hull as substrates. Cultivations were carried out in orbital shaker under microaerophilic conditions, at 30 C and 37 C, for both bacteria. Both hydrolysates presented high osmotic pressures, around 2,000 mOsm/kg, with varying concentrations of glucose, xylose, and arabinose. Both bacteria were able to grow in the hydrolysates, at both temperatures, and they efficiently converted sugars into 2,3-butanediol, showing yields varying from 0.25 to 0.51 g/g of sugars and maximum 2,3-butanediol concentrations varying from 6.4 to 21.9 g/L. Other metabolic products were also obtained in lower amounts, notably ethanol, which peaked at 3.6 g/L in cultures using the enzymatic hydrolysate at 30 C. These results suggest the potential use of these recently isolated bacteria to convert lignocellulosic biomass hydrolysates into value-added products. K E Y W O R D S 2,3-butanediol, bioprocess, Klebsiella pneumoniae, lignocellulosic biomass hydrolysates, Pantoea agglomerans, soybean hull

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Section: Resultsmentioning

confidence: 99%

Production of 2,3‐butanediol byKlebsiella pneumoniaeBLh‐1 andPantoea agglomeransBL1 cultivated in acid and enzymatic hydrolysates of soybean hull

Cortivo

Machado

Hickert

et al. 2019

Biotechnology Progress

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“…Additionally, genetic engineering to decrease by-product formation and to enable production of optically pure 2,3-butanediol will be beneficial [12, 51, 52]. Furthermore, the cultivation medium has to be optimized with respect to utilization of cheaper carbon sources and media components [18, 23, 30, 53, 54].…”

Section: Discussionmentioning

confidence: 99%

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

et al. 2019

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Background Production of 2,3-butanediol from renewable resources is a promising measure to decrease the consumption of fossil resources in the chemical industry. One of the most influential parameters on biotechnological 2,3-butanediol production is the oxygen availability during the cultivation. As 2,3-butanediol is produced under microaerobic process conditions, a well-controlled oxygen supply is the key parameter to control biomass formation and 2,3-butanediol production. As biomass is on the one hand not the final product, but on the other hand the essential biocatalyst, the optimal compromise between biomass formation and 2,3-butanediol production has to be defined. Results A shake flask methodology is presented to evaluate the effects of oxygen availability on 2,3-butanediol production with Bacillus licheniformis DSM 8785 by variation of the filling volume. A defined two-stage cultivation strategy was developed to investigate the metabolic response to different defined maximum oxygen transfer capacities at equal initial growth conditions. The respiratory quotient was measured online to determine the point of glucose depletion, as 2,3-butanediol is consumed afterwards. Based on this strategy, comparable results to stirred tank reactors were achieved. The highest space–time yield (1.3 g/L/h) and a 2,3-butanediol concentration of 68 g/L combined with low acetoin concentrations and avoided glycerol formation were achieved at a maximum oxygen transfer capacity of 13 mmol/L/h. The highest overall 2,3-butanediol concentration of 78 g/L was observed at a maximum oxygen transfer capacity of 4 mmol/L/h. Conclusions The presented shake flask approach reduces the experimental effort and costs providing a fast and reliable methodology to investigate the effects of oxygen availability. This can be applied especially on product and by-product formation under microaerobic conditions. Utilization of the maximum oxygen transfer capacity as measure for the oxygen availability allows for an easy adaption to other bioreactor setups and scales. Electronic supplementary material The online version of this article (10.1186/s12934-019-1126-9) contains supplementary material, which is available to authorized users.

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“…Although the sugar consumption rate slowed down in the initial 12 h and the overall 2,3-BDO productivity decreased, the 2,3-BDO yield from each gram sugar was not jeopardized by decreasing yeast extract and peptone concentrations. Compared to the glucose media, the food waste and its hydrolysate usually contain substances such as minerals and vitamins which are essential nutrients for microbial 2,3-BDO production [ 31 ]. Jurchescu et al (2013) reported a high production of 2,3-BDO using a rich media with various macro- and micro-elements in addition to glucose [ 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…Jurchescu et al (2013) reported a high production of 2,3-BDO using a rich media with various macro- and micro-elements in addition to glucose [ 32 ]. B. licheniformis NCIMB 8059, the strain investigated in the study of Białkowska et al (2015), could not consume any glucose or fructose to produce 2,3-BDO without adding a mineral mixture [ 31 ]. However, when using enzymatic apple pomace hydrolysate (10 g/L) as the media, the yield of 2,3-BDO reached as high as 0.47 g/g.…”

Section: Resultsmentioning

confidence: 99%

Conversion of Food Waste into 2,3-Butanediol via Thermophilic Fermentation: Effects of Carbohydrate Content and Nutrient Supplementation

O’Hair

Poe

et al. 2022

Foods

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Fermentation of food waste into 2,3-butanediol (2,3-BDO), a high-value chemical, is environmentally sustainable and an inexpensive method to recycle waste. Compared to traditional mesophilic fermentation, thermophilic fermentation can inhibit the growth of contaminant bacteria, thereby improving the success of food waste fermentation. However, the effects of sugar and nutrient concentrations in thermophilic food waste fermentations are currently unclear. Here, we investigated the effects of sugar and nutrients (yeast extract (YE) and peptone) concentrations on 2,3-BDO production from fermenting glucose and food waste media using the newly isolated thermophilic Bacillus licheniformis YNP5-TSU. When glucose media was used, fermentation was greatly affected by sugar and nutrient concentrations: excessive glucose (>70 g/L) slowed down the fermentation and low nutrients (2 g/L YE and 1 g/L peptone) caused fermentation failure. However, when food waste media were used with low nutrient addition, the bacteria consumed all 57.8 g/L sugars within 24 h and produced 24.2 g/L 2,3-BDO, equivalent to a fermentation yield of 0.42 g/g. An increase in initial sugar content (72.9 g/L) led to a higher 2,3-BDO titer of 36.7 g/L with a nearly theoretical yield of 0.47 g/g. These findings may provide fundamental knowledge for designing cost-effective food waste fermentation to produce 2,3-BDO.

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Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic Bacillus licheniformis NCIMB 8059

Cited by 28 publications

References 28 publications

Production of 2,3‐butanediol byKlebsiella pneumoniaeBLh‐1 andPantoea agglomeransBL1 cultivated in acid and enzymatic hydrolysates of soybean hull

Production of 2,3‐butanediol byKlebsiella pneumoniaeBLh‐1 andPantoea agglomeransBL1 cultivated in acid and enzymatic hydrolysates of soybean hull

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

Conversion of Food Waste into 2,3-Butanediol via Thermophilic Fermentation: Effects of Carbohydrate Content and Nutrient Supplementation

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