Application of Enzyme-Immobilization Technique for Microflow Reactor

Abstract

The microreaction technology which is an interdisciplinary science and engineering, have attracted attention in many fields in the past years. Several microreactors have been developed. Enzyme is one of the catalysts, which is useful in substance production in an environmentally friendly way, and has high potential for the preparation of chiral compounds. These features are suitable for pharmaceutical process. However, not so many enzymatic processes were commercialized, because of problems in stability of enzyme molecule, cost, and efficiency of the reactions. Thus, there have been demands for innovation in process engineering particularly for enzymatic reactions, and microreaction devices can be a strong tool for the development of enzyme processes. In this minireview, we summarize fundamental immobilization techniques to develop enzyme microreactor. Some important applications of this technology toward the chemical processing are also included.

Immobilization of Laccase on Hybrid Super-Structured Nanomaterials for the Decolorization of Phenolic Dyes

“…The reusability experiments took place in an enzyme-immobilized microreactor at a 2 mL h −1 pumping flow rate. Enzyme-immobilized microreactors are being explored and applied in chemistry and biotechnology fields, as they offer a variety of advantages, such as rapid heat exchange and mass transfer, large surface and interface area, and formation of laminar flow of the streams, which allow strict control of the reaction conditions [62,63].…”

Synergy of Microtechnology and Biotechnology: Microreactors as an Effective Tool for Biotransformation Processes

“…Immobilized enzymes together with microfluidic devices have been applied in recent investigations for various reactions such as hydrolysis and esterification, C-C bond formation reaction, condensation and addition, oxidation and reduction, and polymerization reactions ( 42 ). Additional examples of enzyme immobilization in microreactors are extensively reviewed in the literature ( 10 , 66 , 78 , 84 , 108 - 112 ).…”

Polyethyleneimine-Functionalized Carbon Nanotubes Enabling Potent Antimycotic Activity of Lyticase

“…The selection of loading materials or immobilizing matrices for enzymes have not been well solved and the specific activity of the loaded enzyme is often lower than that of the free enzyme in solution prior to the immobilization [ 15 , 16 , 17 , 18 ]. Considering the particularity of LYT, whose substrate size such as yeast cell is in the range of microns, conventional porous and encapsulation materials are not suitable for carrying LYT.…”