Application of Ex-Vivo/3D Organoid Models in COVID-19 Research

Abstract

COVID-19 treatment methods based on 3D organoids and ex-vivo platforms are analyzed in this chapter. Initially, the platforms available for cell culture and its working characteristics are explained. Subsequently discusses the organoids with their definition and included their uses in various applications. Further, the chapter extends to describe the uses of different organoids with their use in different stages. Most of these methods utilized the 3D ex-vivo cell culture method to develop organoids and test them over infected tissues. Based on the study in this chapter, it is found that the demonstration of active replication of the human organoids culture system of lungs is found to be more helpful for COVID-19 treatment.

In vitro high-content tissue models to address precision medicine challenges

“…These models have been suggested as a prominent platform to gain a fundamental understanding of viral infections such as COVID-19 ( Basanthakumar, 2021 ; de Melo et al, 2021 ; Deguchi et al, 2021 ; Harb et al, 2022 ; Kronemberger et al, 2021 ; Lv et al, 2021 ; Ramani et al, 2021 ; Sia et al, 2022 ; Sourimant et al, 2022 ; van der Vaart et al, 2021 ; Wang et al; Yu, 2021 ; Zech et al, 2021 ) and could be a good alternative to facilitate the development and discovery of new therapeutics and vaccines ( Clevers, 2020 ; Raimondi et al, 2020 ). To date, various types of organoid-enabled 3D models have been employed to study and understand SARS-CoV-2 infection such as the brain organoid ( Ramani et al, 2020 ), lung organoid ( Ekanger et al, 2022 ; Salahudeen et al, 2020 ; van der Vaart et al, 2021 ; Wang et al, 2021a ), intestine organoid ( Dickson, 2020 ), as well as liver ductal organoid and bile duct organoid ( Zhao et al, 2020a ) ( Fig.…”

A deep learning-based approach for predicting COVID-19 diagnosis

“…In [ 6 ], the authors reported that the World Health Organization classified the virus as an epidemic during its early stages, emphasizing its severity. Understanding the potential scale of COVID-19 cases is crucial for effective reaction and management, given the global impact of the pandemic and the absence of an apparent cure.…”