Application of excitation–emission fluorescence matrices and UV/Vis absorption to monitoring the photocatalytic degradation of commercial humic acid

Valencia, Sergio; Marín, Juan; Restrepo, Gloria; Frimmel, Fritz H.

doi:10.1016/j.scitotenv.2012.10.058

Cited by 119 publications

(57 citation statements)

References 42 publications

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“…Humic acid sodium salts (Aldrich) were used for the experiments, as an representative of terrestrial HA (Valencia et al, 2013). They were dissolved in ultrapure water (Barnstead NANOpure system) at a concentration of 1 g/L, to keep ionic strength as low as possible to imitate natural conditions, left on ultrasound bath for 15 minutes and then centrifuged for 30 minutes at 4000 rpm.…”

Section: Methodsmentioning

confidence: 99%

Fluorescence quenching as an indicator of the conformational change of humic<br>acids

Franko

2013

Emir. J. Food Agric

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Humic acids as a water soluble fraction of humus play important role in the plant growth. Even though structure of humic acids is not clear, their spectroscopic properties can reveal us useful information. To investigate influence of humic acids reorganization, HPSEC fractionation at mild fractionation conditions has been carried out. Reconstruction of secondary chromatograms of separated fractions showed molecular size increase and increased response on fluorescence detector, although neither molecular size change nor significant absorbance increase was observed on UV-VIS detector. However, bigger aggregates, that didn't fluoresce in the unfractionated sample started to fluoresce. The reason for this behavior is not some change of spectral properties of a specific fraction, but a general fluorescence increase indicating humic acid reorganization. Therefore spectroscopic changes can be used as a tool for monitoring their reorganization, which might play an important role in nutrients soil plant mobility and should be studied in more details from that perspective.

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Section: Methodsmentioning

confidence: 99%

Fluorescence quenching as an indicator of the conformational change of humic<br>acids

Franko

2013

Emir. J. Food Agric

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“…Since these concentrates had dark colors, they could reduce light transmittance during the EMA treatment. In addition, since humic acids contain many functional groups (e.g., carboxyl and hydroxyl) which can influence the binding and transport of many organic and inorganic compounds [28,29], it is possible that humic acids can also interact with EMA dye and reduce the effective concentration of EMA [14]. On the other hand, the detection results of naked RNA AiV after the EMA treatment were not significantly different from those in the control at any FVs for the IH cases, (p > 0.05).…”

Section: Ema Treatmentmentioning

confidence: 99%

Impact of Various Humic Acids on EMA-RT-qPCR to Selectively Detect Intact Viruses in Drinking Water

Cảnh

Kasuga

Furumai

et al. 2018

J. of Wat. & Envir. Tech.

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To assess the potential risk of viral infection through drinking water, a rapid and effective method to quantify pathogenic viruses is necessary. Ethidium monoazide (EMA) combined with reverse transcription qPCR (EMA-RT-qPCR) is a currently widely accepted method to assess the integrity of viruses. However, this technique can be hampered by humic acids which are co-concentrated during virus concentration processes (VCPs). Co-concentration of four commercially available humic acids (Ald, Wa, Na, and IH) during VCPs and their impacts on the subsequent EMA-RT-qPCR over spiked intact Aichi virus 1 and its naked RNA were studied. The recoveries of Ald, Wa, Na, and IH during VCPs were less than 8.9%, 5.4%, 7.2%, and 0.7%, respectively, indicating that IH was much less concentrated than other humic acids. In the concentrates, Ald, Wa, and Na caused severe inhibition of EMA-RT-qPCR, whereas only a slight inhibitory effect on IH was observed. Tests of the influence of actual drinking water concentrates on EMA-RT-qPCR indicated the absence of severe inhibition. Therefore, EMA-RT-qPCR has a high potential for monitoring enteric viruses in drinking water.

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“…[12][13][14] Combinatorial chemistry is generally conducted in a high-throughput format; further new structures and functions of material by organic combination are generated. 15 The technology of high-throughput experiment shortens the experiment period, improves the efficiency and reduces the costs. [13][14][15] By ink-jet printing, ink is sprayed onto the dielectric surface through a nozzle, which achieves non-contact, high-speed and low-noise printing.…”

Section: 2mentioning

confidence: 99%

“…15 The technology of high-throughput experiment shortens the experiment period, improves the efficiency and reduces the costs. [13][14][15] By ink-jet printing, ink is sprayed onto the dielectric surface through a nozzle, which achieves non-contact, high-speed and low-noise printing. 17,18 The merits of piezoelectric drop-ondemand ink-jet printing are the small size of the ink droplets, high precision and few limitations for ink, which can adapt to all kinds of chemical reagents, in theory.…”

Section: 2mentioning

confidence: 99%

All Ink-jet Printing Strategy for Extensive Exploration of One Chemical Action with Three Interactive Variations

et al. 2017

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Application of excitation–emission fluorescence matrices and UV/Vis absorption to monitoring the photocatalytic degradation of commercial humic acid

Cited by 119 publications

References 42 publications

Fluorescence quenching as an indicator of the conformational change of humic<br>acids

Fluorescence quenching as an indicator of the conformational change of humic<br>acids

Impact of Various Humic Acids on EMA-RT-qPCR to Selectively Detect Intact Viruses in Drinking Water

All Ink-jet Printing Strategy for Extensive Exploration of One Chemical Action with Three Interactive Variations

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