Application of exogenous xyloglucan oligosaccharides affects molecular responses to salt stress in Arabidopsis thaliana seedlings .

Abstract

Soil salinity is one of the most devastating problems which reduces crop production and increases desertification. New approaches to overcome the negative effect of salinity on plants include the use of plant biostimulants, such as Xyloglucan oligosaccharides (XGOs) derived from the breakdown of xyloglucans from plant cell walls. The present study aimed at verifying the influence of exogenous XGOs derived from Tamarindus indica L. cell walls, on Arabidopsis thaliana's tolerance to salt stress by understanding the gene expression, enzymatic and metabolic changes resulting from its application. A. thaliana plants were grown in liquid media and after 15 days they were treated by a salt shock with 100 mM of sodium chloride, with or without XGOs at 0.1 mg L -1 . Gene expression of four oxidative stress markers as well as catalase and peroxidase activities and content of glutathione, total carbonyl, polyphenolics and chlorophyll were quantified. Bioinformatic models were used to obtain the co-expression network of the four oxidative stress response gene markers from microarray data of Arabidopsis under salt stress. Results showed that in saline conditions, XGOs dramatically increased catalase gene expression and enzymatic activity, peroxidase activity, and chlorophyll a/b ratio, while reducing protein oxidation and total polyphenols. Thus, XGOs may act to counteract negative effects of oxidative stress under saline conditions.

Seed Pre-treatment with Polyhydroxy Fullerene Nanoparticles Confer Salt Tolerance in Wheat Through Upregulation of H2O2 Neutralizing Enzymes and Phosphorus Uptake

“…Improvement in salinity tolerance of crops through various exogenous chemicals or biostimulants is an important area (Torbaghan et al 2017;González-Pérez et al 2018). Exposure of wheat seedlings to salinity reduced plant growth.…”

Transcriptomics of Biostimulation of Plants Under Abiotic Stress

“…Using A. thaliana seedlings grown in vitro under salinity stress (100 mM NaCl), researchers (González-Pérez et al, 2018) studied the effect of a biostimulant based on xyloglucan oligosaccharides extracted from Tamarindus indica L. (0.1 mg L −1 ) applied to the growth media. An increase in the expression of the CAT3 gene was reported 24 h after stress, and the CAT gene is discussed above.…”

Effects of Gellan Oligosaccharide and NaCl Stress on Growth, Photosynthetic Pigments, Mineral Composition, Antioxidant Capacity and Antimicrobial Activity in Red Perilla

“…Harmful effects of salinity on plant growth may be reduced by using various growth regulators and biostimulants [24,25,26] that may improve both quantity and quality of crop yield [27,28]. Research studies show that some oligosaccharides used as biostimulants alleviate the negative impacts of stress on plant growth [29,30,31]. It therefore seems interesting to investigate the effects of oligo-gellan in plants exposed to environmental stress.…”