In a previous study, it was shown that dietary supplementation with a carbohydrases and antioxidant-enriched extract (BSG-extract) produced by solid-state fermentation of brewer’s spent grain (BSG) improved nutrients digestibility, feed, and protein utilization of European seabass juveniles. This work aims to investigate the effect of this BSG-extract on liver and intestine oxidative status and muscle lipid peroxidation (LPO) of European seabass. A plant-based diet (55.4% plant feedstuffs; PF) was used as a control. Four test diets were formulated by supplementing the control diet with the BSG-extract (0.4 or 0.8% diet), added directly to the diet (BSG4 and BSG8 diets), or used to pre-treat the PF mixture of the control diet (PreBSG4 and PreBSG8 diets). European seabass juveniles (IBW
22
±
1
g) were fed the experimental diets for 66 days until apparent satiety. In fish fed BSG-extract diets, compared to the control diet, intestinal LPO was reduced up to 29-40% (
p
<
0.05
); GPX, GR, and G6PD activities were increased compared to the control (
p
<
0.05
). In the liver, LPO was similar among diets, but except for catalase and G6PD, fish fed BSG-extract diets had higher antioxidant enzyme activities than the control (
p
<
0.05
). Except for the liver SOD activity, hepatic and intestinal antioxidant capacity was not affected by the dietary BSG-extract incorporation mode (
p
>
0.05
). After 72 h of storage at 4°C, muscle LPO was higher than at time 0 h. Compared to the control, BSG-extract reduced muscle LPO levels up to 30% at time 0 h in fish fed diets BSG4, BSG8, and PreBSG8 (
p
<
0.008
). Results indicate that dietary supplementation with BSG-extract increased the antioxidant level of diets, improved the intestine antioxidant capacity, and decreased LPO in European seabass juveniles’ muscle.