Application of Flow Cytometry in the Diagnosis of Bovine Epidemic Disease

Abstract

As science and technology continue to advance, the use of flow cytometry is becoming more widespread. It can provide important information about cells in the body by detecting and analysing them, thereby providing a reliable basis for disease diagnosis. In the diagnosis of bovine epidemic diseases, flow cytometry can be used to detect bovine viral diarrhoea, bovine leukaemia, bovine brucellosis, bovine tuberculosis, and other diseases. This paper describes the structure of a flow cytometer (liquid flow system, optical detection system, data storage and analysis system) and its working principles for rapid quantitative analysis and sorting of single cells or biological particles. Additionally, the research progress of flow cytometry in the diagnosis of bovine epidemic diseases was reviewed in order to provide a reference for future research and application of flow cytometry in the diagnosis of bovine epidemic diseases.

The role, relevance and management of immune exhaustion in bovine infectious diseases

“…Frequencies and absolute numbers of IFNγ + TNFα + T cells post challenge are evaluated by FACS analysis [ 21 ] in peripheral blood. Similarly recall responses has also been flow cytometrically evaluated in chronic diseases in bovines [ 59 ] .…”