Application of fluorescence lifetime imaging (FLIM) in latent finger mark detection

Seah, Lay Hoon; Wang, P.; Murukeshan, Vadakke Matham; Chao, Z. X.

doi:10.1016/j.forsciint.2005.08.018

Cited by 16 publications

(12 citation statements)

References 8 publications

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“…Besides combining FLIM with FRET to study protein-protein interactions in living cells, FLIM is also effectively combined with various other techniques, including super-resolution microscopy using stimulated emission depletion (STED) [ 221 ], as further discussed below, in virus, yeast and bacteria detection [ 6 ], lifetime-based imaging of fingerprints in forensics [ 222 ], in microfluidic [ 223 , 224 ] and lab-on-chip devices [ 189 ], and other novel biosensors. Excellent reviews on the technical, theoretical, and biological aspects of FLIM can be found in [ 6 , 11 , 39 , 40 , 180 , 182 , 183 , 213 , 216 , 217 , 223 , 225 , 226 , 227 ].…”

Section: Energy Transfer Methods For Inter- and Intra-molecular Inmentioning

confidence: 99%

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

Ishikawa-Ankerhold

Ankerhold

Drummen³

2012

Molecules

450

344

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Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules in biological samples as images or photometric data from which intensities and emission spectra can be deduced. By exploiting the characteristics of fluorescence, various techniques have been developed that enable the visualization and analysis of complex dynamic events in cells, organelles, and sub-organelle components within the biological specimen. The techniques described here are fluorescence recovery after photobleaching (FRAP), the related fluorescence loss in photobleaching (FLIP), fluorescence localization after photobleaching (FLAP), Förster or fluorescence resonance energy transfer (FRET) and the different ways how to measure FRET, such as acceptor bleaching, sensitized emission, polarization anisotropy, and fluorescence lifetime imaging microscopy (FLIM). First, a brief introduction into the mechanisms underlying fluorescence as a physical phenomenon and fluorescence, confocal, and multiphoton microscopy is given. Subsequently, these advanced microscopy techniques are introduced in more detail, with a description of how these techniques are performed, what needs to be considered, and what practical advantages they can bring to cell biological research.

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Section: Energy Transfer Methods For Inter- and Intra-molecular Inmentioning

confidence: 99%

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

Ishikawa-Ankerhold

Ankerhold

Drummen³

2012

Molecules

450

344

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“…1(a)). 10,13,14,24 In the case that there is more than one kind of°uorescent components, for example, di®erent°uorophores or di®erent conformations of a°uorophore, it is necessary to measure the phase shift and the modulation degree at a plurality of modulation frequencies to obtain the°uorescence lifetimes of di®erent°uorescent components. 14 Particularly, the frequency-domain technique can adapt to a wide range of°uorescence intensity variations and tolerate extremely high°uorescence intensity, but in the case of weak°uorescence, the detection e±ciency will drop sharply.…”

Section: Frequency-domain Detection Techniquementioning

confidence: 99%

“…It provides accurate quantitative measurement of living cells and tissues to reveal their biochemical properties. 10,13,14 The combination of these two technologies, TP-FLIM, provides a powerful tool for noninvasive, label-free, structural and functional, intravital imaging of biological systems.…”

Section: Clinical Application Prospect Of Tp-flimmentioning

confidence: 99%

“…It provides an additional dimension of information for°uorescence imaging [10][11][12] and can be exploited for separating biomolecules with largely overlapped spectra but di®erent°uorescence decay speeds. 10,13 Secondly, the transition of°uorophore from the excited state to the ground state is sensitive to its local environment, such as the pH, temperature, oxygen concentration, ion concentration, enzyme activity, and molecular con¯guration. 10,12,14,15 For instance, the°u orescence lifetime of reduced nicotinamide adenine dinucleotide (NADH) changes from hundreds of picoseconds to a few nanoseconds when it was bound to proteins.…”

Section: Introductionmentioning

confidence: 99%

“…10,14 Based on these advantages,°uorescence lifetime imaging microscopy (FLIM) has attracted lots of attention and has been widely used in biophysics and medical diagnosis researches. 13 With complementary advantages, the combination of TPM and FLIM creates a win-win situation. On the one hand, TPM technology provides the pulse laser excitation source which is required for°uorescence lifetime detection.…”

Section: Introductionmentioning

confidence: 99%

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Two-photon excitation fluorescence lifetime imaging microscopy: A promising diagnostic tool for digestive tract tumors

Zhang

et al. 2019

J. Innov. Opt. Health Sci.

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Digestive tract tumors account for 15% and 19.3% of the cancer incidence and deaths, respectively. Early detection of digestive tract tumors is crucial to the reduction of global cancer burden. Two-photon excitation fluorescence lifetime imaging microscopy (TP-FLIM) allows non-invasive, label-free, three-dimensional, high-resolution imaging of living tissues with not only histological but also biochemical characterization ability in both qualitative and quantitative way. Benefiting from these advantages, this technology is promising for clinical diagnosis of digestive tract tumors. In recent years, many efforts have been made in this field and some remarkable progress has been achieved. In this paper, we overview the recent progress of TP-FLIM-based researches on digestive tract tumor detection. Among them, our latest results on the gastric cancer and esophageal cancer are elaborately depicted. Finally, we outlook and discuss the potential advantages and challenges of TP-FLIM in future clinical applications.

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