Application of FT-IR spectroscopy on breast cancer serum analysis

Elmi, Fatemeh; Movaghar, Afshin Fayyaz; Elmi, Maryam; Alinezhad, Heshmatollah; Nikbakhsh, Novin

doi:10.1016/j.saa.2017.06.021

Cited by 106 publications

(63 citation statements)

References 21 publications

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“…The Fourier Transform InfraRed (FTIR) and Raman peak positions in the analyzed cell line and TMV samples with a description of the vibrations corresponding to the respective functional groups[27][28][29][30][31][32][33][34][35][36][37][38][39][40][41][42].…”

mentioning

confidence: 99%

Similarities in the General Chemical Composition of Colon Cancer Cells and Their Microvesicles Investigated by Spectroscopic Methods-Potential Clinical Relevance

Depciuch

Klębowski

Stec

et al. 2020

IJMS

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Colon cancer constitutes 33% of all cancer cases in humans and the majority of patients with metastatic colon cancer still have poor prognosis. An important role in cancer development is the communication between cancer and normal cells. This may occur, among others, through extracellular vesicles (including microvesicles) (MVs), which are being released by both types of cells. MVs may regulate a diverse range of biological processes and are considered as useful cancer biomarkers. Herein, we show that similarity in the general chemical composition between colon cancer cells and their corresponding tumor-derived microvesicles (TMVs) does exist. These results have been confirmed by spectroscopic methods for four colon cancer cell lines: HCT116, LoVo, SW480, and SW620 differing in their aggressiveness/metastatic potential. Our results show that Raman and Fourier Transform InfraRed (FTIR) analysis of the cell lines and their corresponding TMVs did not differ significantly in the characterization of their chemical composition. However, hierarchical cluster analysis of the data obtained by both of the methods revealed that only Raman spectroscopy provides results that are in line with the molecular classification of colon cancer, thus having potential clinical relevance.

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confidence: 99%

Similarities in the General Chemical Composition of Colon Cancer Cells and Their Microvesicles Investigated by Spectroscopic Methods-Potential Clinical Relevance

Depciuch

Klębowski

Stec

et al. 2020

IJMS

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“…By hyperspectral imaging of specific areas of tissue samples, it is possible to achieve a morpho‐chemical correlation between histological and vibrational analysis . The potential offered by FTIR‐HSI to obtain relevant and punctual biomolecular information at molecular level and to detect changes in the biochemical composition and conformation of the most relevant biomolecules on the same sample and at the same time has been already reported in previous studies , investigating prostate , cervix , breast , colon , lung , thyroid and skin tumoral lesions. In some cases, the FTIR‐HSI approach played a key role in detecting early stages of inflammatory and malignant diseases, helping to gain a deeper understanding of the etiologic nature of different pathologies, even before any detectable morphological evidence .…”

Section: Introductionmentioning

confidence: 84%

Investigation of human pancreatic cancer tissues by Fourier Transform Infrared Hyperspectral Imaging

Notarstefano

Conti

Pisani

et al. 2019

Journal of Biophotonics

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Fourier-transform infrared hyperspectral imaging (FTIR-HSI) provides hyperspectral images containing both morphological and chemical information. It is widely applied in the biomedical field to detect tumor lesions, even at the early stage, by identifying specific spectral biomarkers. Pancreatic neoplasms present different prognoses and are not always easily classified by conventional analyses. In this study, tissue samples with diagnosis of pancreatic ductal adenocarcinoma and pancreatic neuroendocrine tumor were analyzed by FTIR-HSI and the spectral data compared with those from healthy and dysplastic samples. Multivariate/univariate approaches were complemented to hyperspectral images, and definite spectral markers of the different lesions identified. The malignant lesions were recognizable both from healthy/dysplastic pancreatic tissues (high values of phospholipids and triglycerides with shorter, more branched and less unsaturated alkyl chains) and between each other (different amounts of total lipids, phosphates and carbohydrates). These findings highlight different metabolic pathways characterizing the different samples, well detectable by FTIR-HSI. K E Y W O R D SFourier transform infrared hyperspectral imaging, multivariate analysis, pancreatic ductal adenocarcinoma, pancreatic neuroendocrine tumors, spectral biomarkers

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“…Different spectroscopic techniques have been used to characterize human breast cancer tissue. Techniques such as magnetic resonance imaging (MRI) [ 1 ], infrared spectroscopy [ 2 , 3 , 4 ], and Raman spectroscopy [ 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ] have been proven to be powerful methods in characterizing and understanding breast tissue. From a chemical point of view, biological specimens consist of complex mixtures of heterogeneous classes of molecules (e.g., water, lipids, proteins, carbohydrates, and nucleic acids).…”

Section: Introductionmentioning

confidence: 99%

Nitrogen-Doped Titanium Dioxide Nanoparticles Modified by an Electron Beam for Improving Human Breast Cancer Detection by Raman Spectroscopy: A Preliminary Study

Surmacki

2020

Diagnostics

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Titanium dioxide (TiO2) is commonly used as a pigment in paints, paper products, polymer compositions, and cosmetic products, and even as a food additive or drug coating material. In recent times, it has also been used in photovoltaic cells, semiconductors, biomedical devices, and air purification. In this paper, the potential application of nitrogen-doped TiO2 nanoparticles modified by an electron beam for improving human breast cancer detection by Raman spectroscopy is presented. Raman spectroscopy (RS) is a promising noninvasive analytical technique in cancer detection that enables us to retrieve a molecular signature of the biochemical composition of cancerous tissue. However, RS still has some challenges in signal detection, mainly related to strong concurrent background fluorescence from the analyzed tissue. The Raman signal scattering is several orders of magnitude smaller than the fluorescence intensity, and strong fluorescence masks a much weaker Raman signal. The Raman results demonstrate that the N-doped TiO2 electron beam-irradiated nanoparticles amplify the Raman scattering. The intrinsic properties of the adsorbed molecules from human breast tissue and the surface properties of the N-doped TiO2 electron beam-irradiated nanoparticles (the excited electron–hole pair at the surface) have a significant effect on the enhanced Raman signal intensity.

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Application of FT-IR spectroscopy on breast cancer serum analysis

Cited by 106 publications

References 21 publications

Similarities in the General Chemical Composition of Colon Cancer Cells and Their Microvesicles Investigated by Spectroscopic Methods-Potential Clinical Relevance

Similarities in the General Chemical Composition of Colon Cancer Cells and Their Microvesicles Investigated by Spectroscopic Methods-Potential Clinical Relevance

Investigation of human pancreatic cancer tissues by Fourier Transform Infrared Hyperspectral Imaging

Nitrogen-Doped Titanium Dioxide Nanoparticles Modified by an Electron Beam for Improving Human Breast Cancer Detection by Raman Spectroscopy: A Preliminary Study

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