Application of FT-Raman spectroscopy to the study of selected organometallic complexes and proteins

Abstract

The study of colored organometallic complexes by dispersive Raman spectroscopy has been limited due to fluorescence or photodecomposition caused by the visible laser used as the excitation source. As a solution to this problem, IT-Raman spectroscopy with a near-infrared laser source has been useful in lowering fluorescence or photolysis in these samples. To investigate the utility of this technique, we have obtained and assigned the FT-Raman spectra of a series of arene chromium tricarbonyl complexes and of cyclopentadienyl manganese tricarbonyl. Some bands previously unobserved by dispersive Raman spectroscopy were seen, including a band assigned to a I3CO satellite in the spectrum of methylbenzoate chromium tricarbonyl. In addition, FT-Raman data for bovine serum albumin (BSA) and Protein-A are presented.

Raman spectroscopy

“…12, JUNE determine unsaturation in natural oils and fats (280), to study photobiological systems (281) and photoactive proteins (282) and its general application to biological materials has been discussed (283). Other applications have related to the study of selected proteins (284), human lenses (285), the analysis of pharamceuticals and biomaterials (286) and studies of human arteries (287). Other instrumental and technique developments relating to biological studies that have been reported are an automated spectrometer for the precise recording of polarized Raman spectra of biopolymers and their components (288), a commercial anesthetic-respiratory gas monitor (289), and the use of confocal Raman spectroscopy to study single living cells and chromosomes ( 290).…”