Application of FTIR Spectroscopy for Quantitative Analysis of Blood Serum: A Preliminary Study

Abstract

The aim of this study was to analyze the possibility of simultaneous determination of the concentration of components from the characteristics of FTIR spectra using the example of a model blood serum. To prepare model solutions, a set of freeze-dried control sera based on bovine blood serum was used, certified for approximately 38 parameters. Based on the values of the absorbance and areas of absorption bands in the FTIR spectra of model solutions, a regression equation was constructed by solving a nonlinear problem using the generalized reduced gradient method. By using the absorbance of the absorption bands at 1717 and 3903 cm−1 and the areas of the absorption bands at 616, 3750, and 3903 cm−1, it is possible to simultaneously determine the concentrations of 38 components with an error of less than 0.1%. The results obtained confirm the potential clinical use of FTIR spectroscopy as a reagent-free express method for the analysis of blood serum. However, its practical implementation requires additional research, in particular, analysis of real blood serum samples and validation of the method.

Using Multistage Energy Barrier of Heterojunctions in Improving Cr(VI) Detection

“…The absorption peaks at 1515 cm −1 and 1627 cm −1 are caused by the stretching vibration of the C=C bonds of quinone and benzene rings separately, while the absorption peaks at 3400 cm −1 are mainly caused by the stretching vibration of N-H bonds in PANI [26,27]. The absorption peak at 3750 cm −1 corresponds to the vibration of the free O-H group [28]. The existence of multiple functional groups in the FTIR spectra further confirms that PANI film was successfully deposited on the Ni/NiO/CeO 2 /Au foam.…”

Smartphone digital image colorimetry for quantification of serum proteins

“…As for infrared spectroscopy, although it has been utilized for serum protein analysis, 50–52 its practical implementation in routine laboratory analysis requires more research to be actualized. 51 Even among other colorimetry methods for protein analysis, such as Bradford assay and bicinchoninic acid assay, the biuret test has been shown to be superior. 49 Because proteins' peptide bonds exhibit high specificity for the biuret reaction, the chromogenic rate is constant for all proteins.…”

“…Thus, the direct UV-Vis spectrophotometric method is not suitable for the analysis of the total protein of some samples especially those whose proteins or peptides have less tyrosine and tryptophan. As for infrared spectroscopy, although it has been utilized for serum protein analysis, [50][51][52] its practical implementation in routine laboratory analysis requires more research to be actualized. 51 Even among other colorimetry methods for protein analysis, such as Bradford assay and bicinchoninic acid assay, the biuret test has been shown to be superior.…”

The effect of the anti‐coagulant EDTA on the deposition and adhesion of whole blood deposits on non‐porous substrates

“…This denaturation process occurs in a fixed pattern (between 5 and 100 h) and thus may be used to estimate the level of denaturation of Hb over time for blood aging estimations, confirming that compositional changes are detectable over time. However, the probability that dislodgement tendencies were induced by compositional changes within blood will need to be investigated further with the aid of chemical analysis of blood using FTIR or Raman spectroscopy [26–32]. For EDTA‐treated blood, the overall trend here showed again an increased dislodgement on smooth surfaces and a decreased dislodgement on rough surfaces.…”