Application of gas chromatography-mass spectrometry-based metabolomics in food science and technology

Putri, Sastia Prama; Ikram, Muhammad Maulana Malikul; Sato, Arisa; Dahlan, Hadi Akbar; Rahmawati, Della; Ohto, Yukina; Fukusaki, Eiichiro

doi:10.1016/j.jbiosc.2022.01.011

Cited by 63 publications

(25 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years, metabolomics has progressed in many fields, and metabolomics is now used widely to evaluate the flavor and nutritional quality of food [ 26 , 27 ]. The main techniques for analyzing volatiles, including gas chromatography–mass spectrometry (GC-MS), two-dimensional gas chromatography (GC × GC), and headspace solid-phase microextraction coupled to gas chromatography–mass spectrometry (HS-SPME/GC-MS), have been applied to determine the chemical compounds that contribute largely to the flavors of many fruits and vegetables [ 28 , 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Guo

Lai

et al. 2022

Metabolites

View full text Add to dashboard Cite

Coconut is a tropical fruit whose flesh has high flavor quality and nutritional value; however, the differences between coconut varieties are still unclear. Here, volatiles and non-volatiles were profiled at three ripening stages by HS-SPME/GC-MS and UHPLC-MS/MS in two coconut varieties (Hainan Tall, HT and Green Dwarf, GD). Four metabolite classes of volatiles were associated with good aroma including hydrocarbons, benzenoids, alcohols and esters, and these volatiles were generally higher in GD, especially at 7 and 9 months of coconut growth. Pathway-based metabolomics revealed that flavonols and their derivatives were significantly enriched in HT, and some of these metabolites were key determinants of HT flesh bitterness, including kaempferol 7-O-glucoside, a known bitter metabolite. Despite the overall accumulation of amino acids, including L-alanine, L-serine and L-methionine in GD, comparative metabolomics revealed that HT flesh provides a higher content of vitamins than GD. This study sheds light on the metabolic pathways and key metabolites differentiating the flesh flavor quality and nutritional value among coconut varieties, and reveals the possible mechanisms of flavor formation and regulation in coconut fruits.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Guo

Lai

et al. 2022

Metabolites

View full text Add to dashboard Cite

show abstract

“…Compared with recent publications, most of the studies focused on analytical techniques by which to determine coffee authenticity for the common types and minimize adulteration [ 37 ]. Several markers were reported for the authenticity evaluation of Luwak coffee, such as caffeine, inositol, and pyroglutamic acid, by using GC-MS [ 38 ] obtained from the robusta Luwak coffee, while citric acid, malic acid, and glycolic acid were reported to be characteristic of arabica Luwak coffee [ 39 ]. Citric acid and malic acid, as compound markers of Luwak coffee [ 2 ], appeared in alignment with those in the NMR spectra evaluation in this study.…”

Section: Discussionmentioning

confidence: 99%

Metabolite Profiling of Premium Civet Luwak Bio-Transformed Coffee Compared with Conventional Coffee Types, as Analyzed Using Chemometric Tools

et al. 2023

View full text Add to dashboard Cite

Luwak (civet) coffee is one of the most precious and exotic coffee commodities in the world. It has garnered an increasing reputation as the rarest and most expensive coffee, with an annual production. Many targeted analytical techniques have been reported for the discrimination of specialty coffee commodities, such as Luwak coffee, from other ordinary coffee. This study presents the first comparative metabolomics approach for Luwak coffee analysis compared to other coffee products, targeting secondary and aroma metabolites using nuclear magnetic resonance (NMR), gas chromatography (GC), or liquid chromatography (LC) coupled with mass spectrometry (MS). Chemometric modeling of these datasets showed significant classification among all samples and aided in identifying potential novel markers for Luwak coffee from other coffee samples. Markers have indicated that C. arabica was the source of Luwak coffee, with several new markers being identified, including kahweol, chlorogenic acid lactones, and elaidic acid. Aroma profiling using solid-phase micro-extraction (SPME) coupled with GC/MS revealed higher levels of guaiacol derivatives, pyrazines, and furans in roasted Luwak coffee compared with roasted C. arabica. Quantification of the major metabolites was attempted using NMR for Luwak coffee to enable future standardization. Lower levels of alkaloids (caffeine 2.85 µg/mg, trigonelline 0.14 µg/mg, and xanthine 0.03 µg/mg) were detected, compared with C. arabica. Other metabolites that were quantified in civet coffee included kahweol and difurfuryl ether at 1.37 and 0.15 µg/mg, respectively.

show abstract

“…In recent years, food safety has received much attention. Pathogens, toxins, and by-products produced by microbial degradation of food are closely related to food safety (Putri et al, 2022). Therefore, monitoring these related metabolites is very important for food safety.…”

Section: Food and Nutritionmentioning

confidence: 99%

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

Meng¹,

LI²,

YANG³

et al. 2023

Biocell

View full text Add to dashboard Cite

In recent years, microbial metabolomics, a new field that has attracted wide attention, provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts. Many microorganisms are found in the human intestine, oral cavity, vagina, etc. Probiotics could maintain the good health of the host, while pathogens and an imbalance of bacterial flora lead to a series of diseases of the body and mind.Metabolomics is a science for qualitative and quantitative analysis of all metabolites in an organism or biological system, which could provide key information to understand the related metabolic pathways and associated changes. This approach analyzes the final products of cellular regulatory processes, the level of which can be regarded as the ultimate response of the biological system to genetic or environmental changes. Microbial metabolomics has been widely used in different research fields, such as microbial phenotypic classification, mutant screening, metabolic pathways, microbial metabolic engineering, fermentation engineering monitoring and optimization, microbial environmental pollution, and so on. However, there are only a few reviews on microbial metabolomics of probiotics and pathogens. This review summarizes the main methodologies, including sample preparation, identification of metabolites, data processing, and analysis. Recent applications in microbial metabolomics of probiotics and pathogens are also described. This paper first summarized the research progress and application of microbial metabolomics from two aspects: probiotics and pathogenic bacteria. Probiotics and pathogenic bacteria do not exist independently most of the time; hence, these were reviewed in the research field of coexistence of probiotics and pathogenic bacteria, which was subdivided into important microbial research fields closely related to human health, including the human gut, oral cavity, food, and nutrition-related microorganisms. Then, the main problems and trends associated with microbial metabolomics are discussed.

show abstract

Application of gas chromatography-mass spectrometry-based metabolomics in food science and technology

Cited by 63 publications

References 64 publications

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Comparative Metabolomics Reveals Key Determinants in the Flavor and Nutritional Value of Coconut by HS-SPME/GC-MS and UHPLC-MS/MS

Metabolite Profiling of Premium Civet Luwak Bio-Transformed Coffee Compared with Conventional Coffee Types, as Analyzed Using Chemometric Tools

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

Contact Info

Product

Resources

About