Application of gender‐ablated semen during timed artificial insemination following oestrous synchronization in dairy and beef cows

Magopa, T. L.; Mphaphathi, M. L.; Mulaudzi, Thendo

doi:10.1111/rda.14323

Cited by 1 publication

(5 citation statements)

References 37 publications

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“…This finding, therefore, necessitates more innovation studies to come up with a sexing technology that will protect sperm cell injuries during sorting, ehich was supported by the evidence from Silva et al's ( 45 ) study in frozen-thawed goats' sperm cells and Guo et al's ( 46 ) study in frozen-thawed cattle's sperm cells. However, the type of insemination used in goats, sheep, and cattle also affects the conception rate following the use of sexed semen ( 12 , 35 , 37 , 41 , 59 ).…”

Section: Discussionmentioning

confidence: 99%

“…An improvement has been observed in cattle both in terms of the use of sexed semen and acceptable conception rate especially in dairy cattle ( 12 ). This improvement might be due to good sperm quality following freezing when compared to other ruminants ( 61 ).…”

Section: Limitations Associated With Sexed Semenmentioning

confidence: 99%

“…Another theory is that, in addition to environmental effects or maybe poor bull selection, pregnancy loss may be attributed to diminished sperm function induced by sex sorting and re-freezing, resulting in low embryo quality. Furthermore, Magopa et al ( 12 ) identified that another factor associated with pregnancy loss in cows could be the use of sexed sperm with sperms that sustained injury during the sorting procedure, which could have resulted in a further reduction in sperm viability in the cow's reproductive tract. Pohler et al ( 64 ) argued that the mechanisms causing pregnancy loss in cows might relate to embryo development or a lack of additional embryonic membrane formation since this happens around the time of embryo attachment and the onset of placentation.…”

Section: Limitations Associated With Sexed Semenmentioning

confidence: 99%

“…The pre-selection of the sex plays an important role in increasing genetic gain and the selection of the desired sex ( 11 ). The sex-sorting concept has sparked a rising interest in calf sex pre-selection using sexed semen in both dairy and beef farmers worldwide where artificial insemination (AI) is used ( 12 ). This process is qualified due to the high demand for heifer calves by the dairy industry to be used as replacement heifers.…”

Section: Introductionmentioning

confidence: 99%

“…Flow cytometry is the only commercialized method of sexing semen and uses LASER to stimulate fluorescent dye that fixes the DNA in spermatozoa. Magopa et al ( 12 ) reported an overall conception rate of 61% and 62% when X-sexed and unsexed sperm were used, respectively, in dairy cows in comparison to 56.0% and 52.2% in beef cows. However, there were embryo losses between days 35 and 65 in dairy cows (X-sexed, 33.3% and unsexed, 18.2%) and beef cows (X-sexed, 28.6% and un-sexed, 29.2%).…”

Section: Introductionmentioning

confidence: 99%

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A systematic review on the prospects of X- and Y-sexed semen in ruminant livestock: implications for conservation, a South African perspective

Ngcobo,

Nedambale,

Sithole

et al. 2024

Front. Vet. Sci.

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South Africa is home to numerous indigenous and locally developed sheep (Nguni Pedi, Zulu, and Namaqua Afrikaner, Afrino, Africander, Bezuidenhout Africander, Damara, Dorper, Döhne Merino, Meat Master, South African Merino, South African Mutton Merino, Van Rooy, and Dorper), goat (SA veld, Tankwa, Imbuzi, Bantu, Boer, and Savanna) and cattle (Afrigus, Afrikaner, Bolowana, Bonsmara, Bovelder, Drakensberger, South African Angus, South African Dairy Swiss, South African Friesland, South African Red, and Veld Master) animals. These breeds require less veterinary service, feed, management efforts, provide income to rural and or poor owners. However, most of them are under extinction risks and some with unknown status hence, require immediate conservation intervention. To allow faster genetic progress on the endangered animals, it is important to generate productive animals while reducing wastages and this can be achieved through sex-sorted semen. Therefore, this systematic review is aimed to evaluate the prospects of X and Y-sexed semen in ruminant livestock and some solutions that can be used to address poor sex-sorted semen and its fertility. This review was incorporated through gathering and assessing relevant articles and through the data from the DAD-IS database. The keywords that were used to search articles online were pre-gender selection, indigenous ecotypes, fertility, flow cytometry, artificial insemination, conservation, and improving sexed semen. Following a careful review of all articles, PRISMA guidelines were used to find the articles that are suitable to address the aim of this review. Sex-sorted semen is a recently introduced technology gaining more attention from researchers particularly, in the conservation programs. Preselection of semen based on the sex chromosomes (X- and or Y-bearing chromosomes) is of paramount importance to obtain desired sex of the offspring and avoid animal wastage as much as possible. However, diverse factors can affect quality of semen of different animal species especially after sex-sorting. Flow cytometry is a common method used to select male and female sperm cells and discard dead and abnormal sperm cells during the process. Thus, sperm sexing is a good advanced reproductive technology (ART) however, it is associated with the production of oxidative stress (OS) and DNA fragmentation (SDF). These findings, therefore, necessitates more innovation studies to come up with a sexing technology that will protect sperm cell injuries during sorting in frozen-thawed.

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