Application of genomics and proteomics for study of the integrated response to zinc exposure in a non-model fish species, the rainbow trout

Högstrand, Christer; Balesaria, Sara; Glover, Chris N.

doi:10.1016/s1096-4959(02)00125-2

Cited by 129 publications

(67 citation statements)

References 51 publications

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“…The application of gene array technology to study effects of environmental contaminants and hypoxia in aquatic systems is in the early stages of development and has been limited to fish (Gracey et al 2001, Hogstrand et al 2001, Larkin et al 2003, Ton et al 2003, Williams et al 2003. The identification of hypoxia-responsive genes and proteins of the blue crab in this study constitutes an important first step toward a better understanding of the benefits and problems associated with the use of gene and protein expression profiles in estuarine invertebrates as molecular indicators of environmental conditions.…”

Section: Discussionmentioning

confidence: 99%

Molecular indicators of hypoxia in the blue crab Callinectes sapidus

Brown‐Peterson¹,

Larkin²,

Denslow³

et al. 2005

Mar. Ecol. Prog. Ser.

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Occurrence of hypoxia in estuarine waters is increasing, and recovery of impacted estuaries is slow. Detection of early effects of hypoxia is needed for timely remedial action to be taken. Here we examine the use of hypoxia-responsive gene and protein expression profiles in the blue crab Callinectes sapidus as early indicators of impacts of hypoxia. We cloned 23 potential hypoxiaresponsive genes, which were used to construct gene macroarrays. Crabs exposed to chronic hypoxia (2.5 ppm dissolved oxygen [DO]) for 15 d showed significant (p < 0.05) changes in gene expression of heat shock protein 70 (Hsp70), copper metallothionein (CuMt3), cytosolic MnSOD (cyt-MnSOD) and ribosomal proteins S15 and L23. In all cases except for CuMt3, gene expression decreased after 5 d exposure to hypoxia. Expression of Hsp70, CuMt3 and cyt-MnSOD also increased (p < 0.05) in normoxic crabs held for 15 d, suggesting confounding effects from confinement stress. Hemocyanin protein concentrations changed significantly (p = 0.005) across the 15 d chronic hypoxia exposure. Hemocyanin in crabs exposed to 10 d intermittent hypoxia (2.5 to 8 ppm DO over a 24 h cycle) did not change, but cyt-MnSOD gene expression increased significantly (p = 0.037), whereas cytochrome c oxidase subunit 1 (ccox1) showed a 2.2-fold downregulation. Blue crabs collected from Pensacola Bay, Florida, showed significant (p < 0.006) downregulation of ccox1 and cyt-MnSOD gene expression as well as hemocyanin protein levels at a diurnally hypoxic marsh site. Several hypoxiaresponsive genes (elongation factor 2, cryptocyanin, hemocyanin) were also significantly elevated (p < 0.006) in intermolt versus premolt normoxic crabs. The identification of hypoxia-responsive genes and proteins in the blue crab is a promising first step towards the development of sensitive molecular tools for the detection of sublethal effects of hypoxia in estuarine-resident species.KEY WORDS: Dissolved oxygen · Hypoxia · Crustacea · Molecular biomarker · Gene arrays Resale or republication not permitted without written consent of the publisher

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Section: Discussionmentioning

confidence: 99%

Molecular indicators of hypoxia in the blue crab Callinectes sapidus

Brown‐Peterson¹,

Larkin²,

Denslow³

et al. 2005

Mar. Ecol. Prog. Ser.

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“…This all could in turn cause major impairments in energy metabolism and increase the ROS burden that finally may submit cells to apoptosis or necrosis (Provinciali et al 2002;Rudolf et al 2003). Since epithelial cells are primarily affected by an excess intake of zinc in vivo (Hogstrand et al 2002) we have used the human colon adenocarcinoma cell line HT-29 as an in vitro model to study the response to a high zinc exposure. This cell line has also been used recently to identify differentially expressed genes under zinc-deficient conditions (Kindermann et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Effects of increased cellular zinc levels on gene and protein expression in HT-29 cells

et al. 2005

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High cellular zinc concentrations lead to impairments in ATP synthesis and cell cycle control particularly in neurons and epithelial cells. The molecular basis for these dysfunctions is still not fully elucidated. Here we analyzed the effects of a high zinc exposure (10 ppm) on gene and protein expression in the human epithelial cell line HT-29. Of the 1176 genes analyzed with cDNA arrays, nine differentially expressed genes were identified. Proteome analysis based on 1310 detected proteins identified 11 molecular targets. Most of the identified genes/proteins have not been linked to cellular zinc status before (e.g. PEC-60, R-ras3). More than half of the targets participate in ATP production or stress response. Therefore, it appears that higher zinc concentrations mediate their effects mainly via impairments in cellular energy metabolism and stress response.

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“…There are a number of laboratory-based exposures for proteomics, using diverse fish species [41][42][43][44]. However, there are comparatively fewer for field studies.…”

Section: Proteomic Field-based Studiesmentioning

confidence: 99%

Defining the role of omics in assessing ecosystem health: Perspectives from the Canadian environmental monitoring program

Bahamonde

Feswick

Isaacs

et al. 2015

Enviro Toxic and Chemistry

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Scientific reviews and studies continue to describe omics technologies as the next generation of tools for environmental monitoring, while cautioning that there are limitations and obstacles to overcome. However, omics has not yet transitioned into national environmental monitoring programs designed to assess ecosystem health. Using the example of the Canadian Environmental Effects Monitoring (EEM) program, the authors describe the steps that would be required for omics technologies to be included in such an established program. These steps include baseline collection of omics endpoints across different species and sites to generate a range of what is biologically normal within a particular ecosystem. Natural individual variability in the omes is not adequately characterized and is often not measured in the field, but is a key component to an environmental monitoring program, to determine the critical effect size or action threshold for management. Omics endpoints must develop a level of standardization, consistency, and rigor that will allow interpretation of the relevance of changes across broader scales. To date, population-level consequences of routinely measured endpoints such as reduced gonad size or intersex in fish is not entirely clear, and the significance of genome-wide molecular, proteome, or metabolic changes on organism or population health is further removed from the levels of ecological change traditionally managed. The present review is not intended to dismiss the idea that omics will play a future role in large-scale environmental monitoring studies, but rather outlines the necessary actions for its inclusion in regulatory monitoring programs focused on assessing ecosystem health. Environ Toxicol Chem 2016;35:20-35. # 2015 SETAC

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Application of genomics and proteomics for study of the integrated response to zinc exposure in a non-model fish species, the rainbow trout

Cited by 129 publications

References 51 publications

Molecular indicators of hypoxia in the blue crab Callinectes sapidus

Molecular indicators of hypoxia in the blue crab Callinectes sapidus

Effects of increased cellular zinc levels on gene and protein expression in HT-29 cells

Defining the role of omics in assessing ecosystem health: Perspectives from the Canadian environmental monitoring program

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