Application of genomics and proteomics in drug target discovery

Zhang, H M; Nan, Zhirun; Hui, Guoqiang; Liu, X H; Sun, Yuqiang

doi:10.4238/2014.january.10.11

Cited by 7 publications

(6 citation statements)

References 18 publications

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“…Data obtained from genome analysis and transcription studies of specific genes are not sufficient to ensure high active protein yield. The expression "one gene, one protein" is not accurate, as it has been confirmed that the average number of protein isoforms per gene is one or two in bacteria, three in yeast, and three or more in humans because of the formation of protein isoforms produced by different forms of posttranslational modifications (phosphorylation, glycosylation, nitrosation, ethylation, and methylation) [20]. Bioinformatics analysis may reveal potential posttranslational modifications in certain proteins.…”

Section: Drug Design and Discoverymentioning

confidence: 99%

“…Proteomics studies have broadened the perspective of protein research to examine the consequences of protein function in establishing the phenotype and interaction of a drug with its target. These studies have also revealed the pathophysiological basis of disease, validation of drug targets, action, toxicity, and side effects [20].…”

Section: Drug Design and Discoverymentioning

confidence: 99%

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Bioinformatics, Genomics, and Proteomics Tools in Drug Design

Fs¹

2019

J Drug Res Dev

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Unraveling of human genome sequences had enabled the discovery of new drugs, studies of their effects, and determination of their main targets. Integrated genomics, proteomics, and bioinformatics have led to powerful new strategies for solving many biochemical issues and developing new techniques which give new biomedical products. Among the applications were analyses of DNA and genome annotation, predicting target structure, and understanding the genetics of disease. As a result, a new trend in research had evolved to illustrate the mechanism of drug action, predict of drug resistance, and discover biomarkers for many diseases. The discovery of new drugs is complex process. Drug design is based on 2 main strategies: ligand-based drug design and structure-based drug design. Studying protein structure and function was the key to develop drug in which modern techniques were used based on combinatorial approaches like proteomics, genomics, bioinformatics, molecular docking and mass spectrometry. To successfully design a new drug, it is important to identify the cell or tissue targeted by this drug. No absolute confirmation that the suspected drug will be effective in vivo. In this review, an overview on the combinatorial approaches of proteomics, genomics and bioinformatics that help understanding the process of drug design is discussed.

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Section: Drug Design and Discoverymentioning

confidence: 99%

Section: Drug Design and Discoverymentioning

confidence: 99%

Bioinformatics, Genomics, and Proteomics Tools in Drug Design

Fs¹

2019

J Drug Res Dev

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“…Além disso, uma investigação das principais vias bioquímicas possibilita a identificação de proteínas essenciais para o crescimento e desenvolvimento do micro-organismo alvo. Já uma análise comparativa dos organismos pode levar, preferencialmente, à seleção de proteínas ausentes no hospedeiro ou então com importantes diferenças estruturais em relação às proteínas que desempenham função análoga em humanos, culminando em ligantes seletivos e minimizando problemas como inespecificidade e toxicidade (Zhang et al 2014) Esta revisão concentrará nas abordagens quimioproteômicas utilizadas para a descoberta de alvos de fármacos e rastreamento de compostos com potencial terapêutico, como cromatografia de afinidade, perfil de proteínas baseado na atividade e estabilidade, imobilização de moléculas usando microarranjo de proteínas e rastreio virtual.…”

Section: Introductionunclassified

Dna Barcoding Confirma a Ocorrência De Espécies Amazônicas Na Ictiofauna Do Rio Turiaçu, Maranhão/Brasil

Teixeira¹,

Barros²,

Fraga³

2019

Conceitos Básicos Da Genética

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“…Literature survey reveals that several analytical methods have been reported for the determination of miconazole nitrate (MIC) alone or in combinations including spectrophotometry [3][4][5][6][7][8][9][10][11], spectrofluorometry [4] and HPLC [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30]. Besides, several analytical methods have been reported for the determination of hydrocortisone acetate (HYD) alone or in combination with other drugs including spectrophotometry [31][32][33][34][35], and HPLC [36][37][38][39][40][41][42][43][44][45].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of miconazole and hydrocortisone or mometasone using reversed phase liquid chromatography

et al. 2012

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KEYWORDSTwo simple, rapid and precise reversed phase liquid chromatographic methods have been developed and validated for the simultaneous determination of miconazole nitrate in two binary mixtures, with hydrocortisone acetate (Mixture 1) and mometasone furoate (Mixture 2). For the two mixtures, chromatographic separation was carried out on a C18 column. For mixture 1, a mobile phase consisting of 2.22 mM sodium dihydrogen phosphate (Triethylamine 0.2%):acetonitrile (45:55, v:v) at a flow rate of 0.9 mL/min was used at ambient temperature. Quantitative determination of miconazole and hydrocortisone was achieved with UV detection at 215 and 245 nm, respectively. Linearity, accuracy and precision were found to be acceptable over the concentration range of 30-80 μg/mL for miconazole and 4-80 μg/mL for hydrocotisone. For mixture 2, a mobile phase consisting of acetonitrile:water (Triethylamine 0.2%) (70:30, v:v) at a flow rate of 0.9 mL/min was used at ambient temperature. Quantitative determinations of miconazole and mometasone were achieved with UV detection at 215 and 250 nm, respectively. Linearity, accuracy and precision were found to be acceptable over the concentration range of 10-200 μg/mL for miconazole and 2-60 μg/mL for mometasone. The optimized methods were proved to be specific, robust and accurate for the quality control of the cited drugs in pharmaceutical preparations.

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Application of genomics and proteomics in drug target discovery

Cited by 7 publications

References 18 publications

Bioinformatics, Genomics, and Proteomics Tools in Drug Design

Bioinformatics, Genomics, and Proteomics Tools in Drug Design

Dna Barcoding Confirma a Ocorrência De Espécies Amazônicas Na Ictiofauna Do Rio Turiaçu, Maranhão/Brasil

Simultaneous determination of miconazole and hydrocortisone or mometasone using reversed phase liquid chromatography

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