Application of gold nanoparticles as contrast agents in confocal laser scanning microscopy

Lemelle, Arnaud; Veksler, B A; Kozhevnikov, Ilya O.; Akchurin, Garif G.; Piletsky, Sergey A.; Meglinski, Igor

doi:10.1002/lapl.200810091

Cited by 40 publications

(18 citation statements)

References 29 publications

(45 reference statements)

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“…[19]. In addition, the sensitivity of far-field quantities such as the scattering efficiency to the radial anisotropy may be used for several potential applications including remote controlling, designing sensors, and enhancing the quality of confocal laser scanning microscopy images [30], etc.…”

Section: Resultsmentioning

confidence: 99%

Plasmonic Resonant Light Scattering by a Cylinder With Radial Anisotropy

Jin¹,

Gao²,

Gao³

2010

PIER

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Abstract-We apply the full-wave electromagnetic theory to study electromagnetic scattering by a small cylindrical particle with radial anisotropy for normally incident light with transverse magnetic (TM) polarization. The scattering coefficients are derived, when the radial anisotropies in both the permittivity and permeability tensors are taken into account. It is shown that the surface and volume plasmon resonances can be identified by the sign of dε t /dq, in which ε t is the permittivity element in a direction tangential to the local r-axis, and q is the size parameter. The near field distributions for surface and volume modes are illustrated by finite element method. It is found that small changes of anisotropy can affect the scattering efficiencies significantly. Moreover, the quadrupole and octupole resonant peaks may be much higher and sharper than those of dipole resonance in the scattering efficiency spectra.

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Section: Resultsmentioning

confidence: 99%

Plasmonic Resonant Light Scattering by a Cylinder With Radial Anisotropy

Jin¹,

Gao²,

Gao³

2010

PIER

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“…3,9,17 However, most of the observation of gold nanoparticles inside cells are in 2D rather than in 3D. 1,21,33,44 In this section, we show dual-mode OPTM can image 3D distribution of gold nanorods 18 inside a single cancer cell.…”

Section: Polarization/absorption Imagingmentioning

confidence: 97%

Multimodal 3D Imaging of Cells and Tissue, Bridging the Gap Between Clinical and Research Microscopy

et al. 2011

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Absorption dyes are widely used in traditional cytology and pathology clinical practice, while fluorophores and nanoparticles are more often used in biologic research. Optical projection tomographic microscopy (OPTM) is a platform technology that can image the same specimen in multiple modes in 3D, providing morphologic and molecular information concurrently and in exact co-registration. The depth-of-field of a high numerical aperture objective is extended by scanning the focal plane through the sample to generate an optical projection image. Samples of cells or tissue are brought into the OPTM instrument through a microcapillary tube filled with optical index-matching gel. Multiple optical projection images are taken from different perspectives by rotating the tube. Computed tomography (CT) algorithms are applied to these optical projection images to reconstruct 3D structure of the sample. Image segmentation and analysis based on these 3D images provide quantitative biosignatures for cancer diagnosis that represents a clear improvement over conventional 2D image analysis. In this article, we introduce the OPTM platform, optical Cell-CT, and Tissue-CT instruments, and some applications using these OPTM instruments.

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“…To circumvent dye leakage, intrinsic fluorescence of nanoparticles is an attractive alternative, as has been described for gold. 20,21 To study the entry route of a specific ENM, physical and pharmacological blockers may be used. All methods used to induce a physical or pharmacological block of uptake pathways have certain limitations.…”

Section: General Considerationsmentioning

confidence: 99%